Pip, a novel activator of phenazine biosynthesis in Pseudomonas chlororaphis PCL1391

[en] Secondary metabolites are important factors for interactions between bacteria and other organisms. Pseudomonas chlororaphis PCL1391 produces the antifungal secondary metabolite phenazine-1-carboxamide (PCN) that inhibits growth of Fusarium oxysporum f. sp. radius lycopersici the causative agent of tomato foot and root rot. Our previous work unraveled a cascade of genes regulating the PCN biosynthesis operon, phzABCDEFGH. Via a genetic screen, we identify in this study a novel TetR/AcrR regulator, named Pip (phenazine inducing protein), which is essential for PCN biosynthesis. A combination of a phenotypical characterization of a pip mutant, in trans complementation assays of various mutant strains, and electrophoretic mobility shift assays identified Pip as the fifth DNA-binding protein so far involved in regulation of PCN biosynthesis. In this regulatory pathway, Pip is positioned downstream of PsrA (Pseudomonas sigma factor regulator) and the stationary-phase sigma factor RpoS, while it is upstream of the quorum-sensing system PhzI/PhzR. These findings provide further evidence that the path leading to the expression of secondary metabolism gene clusters in Pseudomonas species is highly complex.

Disciplines :

Biochemistry, biophysics & molecular biology
Microbiology

Author, co-author :

Girard, Geneviève

Barends, S.

Rigali, Sébastien ; Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie

van Rij, E. T.

Lugtenberg, B. J.

Bloemberg, G. V.

Language :

English

Title :

Pip, a novel activator of phenazine biosynthesis in Pseudomonas chlororaphis PCL1391

Publication date :

2006

Journal title :

Journal of Bacteriology

ISSN :

0021-9193

eISSN :

1098-5530

Publisher :

American Society for Microbiology (ASM), Washington, United States - District of Columbia

Volume :

188

Issue :

Pages :

8283-8293

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 17 September 2009

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Bibliography

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