[en] In mitochondrial oxidative phosphorylation, electron transfer from NADH or succinate to oxygen by a series of large protein complexes in the inner mitochondrial membrane (complexes I-IV) is coupled to the generation of an electrochemical proton gradient, the energy of which is utilized by complex V to generate ATP. In Euglena gracilis, a non-parasitic secondary green alga related to trypanosomes, these respiratory complexes totalize more than 40 Euglenozoa-specific subunits along with about 50 classical subunits described in other eukaryotes. In the present study the Euglena proton-pumping complexes I, III, and IV were purified from isolated mitochondria by a two-steps liquid chromatography approach. Their atypical subunit composition was further resolved and confirmed using a three-steps PAGE analysis coupled to mass spectrometry identification of peptides. The purified complexes were also observed by electron microscopy followed by single-particle analysis. Even if the overall structures of the three oxidases are similar to the structure of canonical enzymes (e.g. from mammals), additional atypical domains were observed in complexes I and IV: an extra domain located at the tip of the peripheral arm of complex I and a "helmet-like" domain on the top of the cytochrome c binding region in complex IV.
Miranda Astudillo, Héctor Vicente ; Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues
Yadav, Sathish; University of Groningen > Groningen Biological Sciences and Biotechnology Institute > Department ofElectron Microscopy
Colina-Tenorio, Lilia; Universidad Nacional Autónoma de México > Instituto de Fisiología Celular > Departamento de Genética Molecular
Bouillenne, Fabrice ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines
Degand, Hervé; Université Catholique de Louvain, Louvain-la-Neuve > Institut des Sciences de la Vie
Morsomme, Pierre; Université Catholique de Louvain, Louvain-la-Neuve > Institut des Sciences de la Vie
Boekema, Egbert; University of Groningen > Groningen Biological Sciences and Biotechnology Institute > Department ofElectron Microscopy
Cardol, Pierre ; Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues
Language :
English
Title :
The atypical subunit composition of respiratory complexes I and IV is associated with original extra structural domains in Euglena gracilis.
Publication date :
2018
Journal title :
Scientific Reports
eISSN :
2045-2322
Publisher :
Nature Publishing Group, London, United Kingdom
Volume :
8
Issue :
1
Pages :
9698
Peer reviewed :
Peer Reviewed verified by ORBi
European Projects :
H2020 - 682580 - BEAL - Bioenergetics in microalgae : regulation modes of mitochondrial respiration, photosynthesis, and fermentative pathways, and their interactions in secondary algae
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