Reference : Enantioresolution of basic pharmaceuticals using cellulose tris(4-chloro-3-methylphen...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
Enantioresolution of basic pharmaceuticals using cellulose tris(4-chloro-3-methylphenylcarbamate) as chiral stationary phase and polar organic mobile phases.
Dossou, Katina Sourou Sylvestre [Université de Liège - ULiège > > > Doct. sc. bioméd. & pharma. (Bologne)]
Chiap, Patrice [Centre Hospitalier Universitaire de Liège - CHU > > Pharmacologie clinique >]
Chankvetadze, Bezhan [University of Munster > > > > > >]
Servais, Anne-Catherine mailto [Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments >]
Fillet, Marianne mailto [Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments >]
Crommen, Jacques mailto [Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments >]
Journal of Chromatography. A
Elsevier Science
Yes (verified by ORBi)
The Netherlands
[en] Chiral basic pharmaceuticals ; Polar organic solvent chromatography ; Acidic additives ; Screening
[en] A polysaccharide-based chiral stationary phase (Sepapak-4), with cellulose tris(4-chloro-3-methylphenylcarbamate) as chiral selector, has been investigated in liquid chromatography (LC). Its enantioresolution power was evaluated towards 13 basic amino-drugs with widely different structures and polarities, using polar organic mobile phases. After preliminary experiments, acetonitrile was selected as the main mobile phase component, to which a low concentration of diethylamine (0.1%) was systematically added in order to obtain efficient and symmetrical peaks. Different organic solvents were first added in small proportions (5-10%) to acetonitrile to modulate analyte retention. Polar organic modifiers were found to decrease retention and enantioresolution while hexane had the opposite effect, indicating normal-phase behaviour under these conditions. The addition of an organic acid (formic, acetic or trifluoroacetic acid) was found to strongly influence the retention of the basic amino drugs in these nonaqueous systems. The nature and proportion of the acidic additive in the mobile phase had also deep impact on enantioresolution. Therefore, the studied compounds could be subdivided in three groups in respect to the acidic additive used. All analytes could be enantioseparated in relatively short analysis times (10-20min) using these LC conditions.
FNRS; Belgian Science Policy Office (SPO)
Researchers ; Professionals ; Students

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