[en] Sea cucumbers produce saponins as a chemical defense mechanism, however their cells can tolerate the cytotoxic nature of these chemicals. To elucidate the molecular mechanisms behind this tolerance a suite of complementary biophysical tools was used, firstly using liposomes for in vitro techniques then using in silico approaches for a molecular-level insight. The holothuroid saponin Frondoside A, caused significantly less permeabilization in liposomes containing a Δ7 holothuroid sterol than those containing cholesterol and resulted in endothermic interactions versus exothermic interactions with cholesterol containing liposomes. Lipid phases simulations revealed that Frondoside A has an agglomerating effect on cholesterol domains, however, induced small irregular Δ7 sterol clusters. Our results suggest that the structural peculiarities of holothuroid sterols provide sea cucumbers with a mechanism to mitigate the sterol-agglomerating effect of saponins, and therefore to protect their cells from the cytotoxicity of the saponins they produce.
Disciplines :
Biochimie, biophysique & biologie moléculaire
Auteur, co-auteur :
Claereboudt, Emily ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
Eeckhaut, Igor; Université de Mons - UMONS
Lins, Laurence ✱; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
Deleu, Magali ✱; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
✱ Ces auteurs ont contribué de façon équivalente à la publication.
Langue du document :
Anglais
Titre :
How different serols contribute to saponin tolerant plasma membranes in sea cucumbers
Date de publication/diffusion :
2018
Titre du périodique :
Scientific Reports
eISSN :
2045-2322
Maison d'édition :
Nature, Royaume-Uni
Volume/Tome :
8
Pagination :
10845
Peer reviewed :
Peer reviewed vérifié par ORBi
Organisme subsidiant :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture
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