Article (Scientific journals)
How different serols contribute to saponin tolerant plasma membranes in sea cucumbers
Claereboudt, Emily; Eeckhaut, Igor; Lins, Laurence et al.
2018In Scientific Reports, 8, p. 10845
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Keywords :
saponin; membrane
Abstract :
[en] Sea cucumbers produce saponins as a chemical defense mechanism, however their cells can tolerate the cytotoxic nature of these chemicals. To elucidate the molecular mechanisms behind this tolerance a suite of complementary biophysical tools was used, firstly using liposomes for in vitro techniques then using in silico approaches for a molecular-level insight. The holothuroid saponin Frondoside A, caused significantly less permeabilization in liposomes containing a Δ7 holothuroid sterol than those containing cholesterol and resulted in endothermic interactions versus exothermic interactions with cholesterol containing liposomes. Lipid phases simulations revealed that Frondoside A has an agglomerating effect on cholesterol domains, however, induced small irregular Δ7 sterol clusters. Our results suggest that the structural peculiarities of holothuroid sterols provide sea cucumbers with a mechanism to mitigate the sterol-agglomerating effect of saponins, and therefore to protect their cells from the cytotoxicity of the saponins they produce.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Claereboudt, Emily ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
Eeckhaut, Igor;  Université de Mons - UMONS
Lins, Laurence   ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
Deleu, Magali   ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
 These authors have contributed equally to this work.
Language :
English
Title :
How different serols contribute to saponin tolerant plasma membranes in sea cucumbers
Publication date :
2018
Journal title :
Scientific Reports
eISSN :
2045-2322
Publisher :
Nature Publishing Group, United Kingdom
Volume :
8
Pages :
10845
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
Available on ORBi :
since 19 June 2018

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