Accelerated ex situ breeding of GBSS- and PTST1-edited cassava for modified starch

[en] Crop diversification required to meet demands for food security and industrial use is often challenged by breeding time and amenability of varieties to genome modification. Cassava is one such crop. Grown for its large starch-rich storage roots, it serves as a staple food and a commodity in the multibillion-dollar starch industry. Starch is composed of the glucose polymers amylopectin and amylose, with the latter strongly influencing the physicochemical properties of starch during cooking and processing. We demonstrate that CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9)–mediated targeted mutagenesis of two genes involved in amylose biosynthesis, PROTEIN TARGETING TO STARCH (PTST1) or GRANULE BOUND STARCH SYNTHASE (GBSS), can reduce or eliminate amylose content in root starch. Integration of the Arabidopsis FLOWERING LOCUS T gene in the genome-editing cassette allowed us to accelerate flowering—an event seldom seen under glasshouse conditions. Germinated seeds yielded S1, a transgene-free progeny that inherited edited genes. This attractive new plant breeding technique for modified cassava could be extended to other crops to provide a suite of novel varieties with useful traits for food and industrial applications.

Disciplines :

Biotechnology

Author, co-author :

Bull, Simon

Seung, David

Chanez, Christelle

Mehta, Devang

Kuon, Joel-Elias

Truernit, Elisabeth

Hochmuth, Anton

Zurkirchen, Irene

Zeeman, Sam

Gruissem, Wilhlem

Vanderschuren, Hervé ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Ingénierie des productions végétales et valorisation

Language :

English

Title :

Accelerated ex situ breeding of GBSS- and PTST1-edited cassava for modified starch

Publication date :

05 September 2018

Journal title :

Science Advances

eISSN :

2375-2548

Publisher :

American Association for the Advancement of Science (AAAS), Washington, United States - District of Columbia

Volume :

Pages :

eaat6086

Peer reviewed :

Peer Reviewed verified by ORBi

European Projects :

H2020 - 713683 - COFUNDfellowsDTU - H.C. Ørsted Fellows Programme – co-funded by Marie Skłodowska Curie Actions (COFUNDfellowsDTU)

Funders :

CE - Commission Européenne

Available on ORBi :

since 18 June 2018

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