Reference : Accelerated ex situ breeding of GBSS- and PTST1-edited cassava for modified starch
Scientific journals : Article
Life sciences : Biotechnology
Accelerated ex situ breeding of GBSS- and PTST1-edited cassava for modified starch
Bull, Simon [> >]
Seung, David [> >]
Chanez, Christelle [> >]
Mehta, Devang [> >]
Kuon, Joel-Elias [> >]
Truernit, Elisabeth [> >]
Hochmuth, Anton [> >]
Zurkirchen, Irene [> >]
Zeeman, Sam [> >]
Gruissem, Wilhlem [> >]
Vanderschuren, Hervé mailto [Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Ingénierie des productions végétales et valorisation >]
Science Advances
Yes (verified by ORBi)
United States
[en] breeding ; genome editing ; crop
[en] Crop diversification required to meet demands for food security and industrial use is often challenged by
breeding time and amenability of varieties to genome modification. Cassava is one such crop. Grown for its large
starch-rich storage roots, it serves as a staple food and a commodity in the multibillion-dollar starch industry.
Starch is composed of the glucose polymers amylopectin and amylose, with the latter strongly influencing the
physicochemical properties of starch during cooking and processing. We demonstrate that CRISPR-Cas9 (clustered
regularly interspaced short palindromic repeats/CRISPR-associated protein 9)–mediated targeted mutagenesis of
two genes involved in amylose biosynthesis, PROTEIN TARGETING TO STARCH (PTST1) or GRANULE BOUND STARCH
SYNTHASE (GBSS), can reduce or eliminate amylose content in root starch. Integration of the Arabidopsis
FLOWERING LOCUS T gene in the genome-editing cassette allowed us to accelerate flowering—an event seldom
seen under glasshouse conditions. Germinated seeds yielded S1, a transgene-free progeny that inherited edited
genes. This attractive new plant breeding technique for modified cassava could be extended to other crops to
provide a suite of novel varieties with useful traits for food and industrial applications.
Researchers ; General public
H2020 ; 713683 - COFUNDfellowsDTU - H.C. Ørsted Fellows Programme – co-funded by Marie Skłodowska Curie Actions (COFUNDfellowsDTU)

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