Article (Scientific journals)
Accelerated ex situ breeding of GBSS- and PTST1-edited cassava for modified starch
Bull, Simon; Seung, David; Chanez, Christelle et al.
2018In Science Advances, 4, p. 6086
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Keywords :
breeding; genome editing; crop
Abstract :
[en] Crop diversification required to meet demands for food security and industrial use is often challenged by breeding time and amenability of varieties to genome modification. Cassava is one such crop. Grown for its large starch-rich storage roots, it serves as a staple food and a commodity in the multibillion-dollar starch industry. Starch is composed of the glucose polymers amylopectin and amylose, with the latter strongly influencing the physicochemical properties of starch during cooking and processing. We demonstrate that CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9)–mediated targeted mutagenesis of two genes involved in amylose biosynthesis, PROTEIN TARGETING TO STARCH (PTST1) or GRANULE BOUND STARCH SYNTHASE (GBSS), can reduce or eliminate amylose content in root starch. Integration of the Arabidopsis FLOWERING LOCUS T gene in the genome-editing cassette allowed us to accelerate flowering—an event seldom seen under glasshouse conditions. Germinated seeds yielded S1, a transgene-free progeny that inherited edited genes. This attractive new plant breeding technique for modified cassava could be extended to other crops to provide a suite of novel varieties with useful traits for food and industrial applications.
Disciplines :
Biotechnology
Author, co-author :
Bull, Simon
Seung, David
Chanez, Christelle
Mehta, Devang
Kuon, Joel-Elias
Truernit, Elisabeth
Hochmuth, Anton
Zurkirchen, Irene
Zeeman, Sam
Gruissem, Wilhlem
Vanderschuren, Hervé  ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Ingénierie des productions végétales et valorisation
Language :
English
Title :
Accelerated ex situ breeding of GBSS- and PTST1-edited cassava for modified starch
Publication date :
05 September 2018
Journal title :
Science Advances
eISSN :
2375-2548
Publisher :
American Association for the Advancement of Science (AAAS), Washington, United States - District of Columbia
Volume :
4
Pages :
eaat6086
Peer reviewed :
Peer Reviewed verified by ORBi
European Projects :
H2020 - 713683 - COFUNDfellowsDTU - H.C. Ørsted Fellows Programme – co-funded by Marie Skłodowska Curie Actions (COFUNDfellowsDTU)
Funders :
CE - Commission Européenne [BE]
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