Reference : The severe deficiency of the somatotrope GHRH/GH/IGF1 axis of Ghrh-/- mice is associa...
Scientific journals : Article
Human health sciences : Endocrinology, metabolism & nutrition
Human health sciences : Immunology & infectious disease
The severe deficiency of the somatotrope GHRH/GH/IGF1 axis of Ghrh-/- mice is associated with an important splenic atrophy and relative B lymphopenia
Bodart, Gwennaëlle mailto [Centre Hospitalier Universitaire de Liège - CHU > > Centre d'oncologie >]
Farhat, Khalil mailto [Université de Liège - ULiège > > Centre d'immunologie >]
Charlet-Renard, Jeanne de Chantal mailto [Centre Hospitalier Universitaire de Liège - CHU > > Service d'endocrinologie clinique >]
Becker, Guillaume mailto [Université de Liège - ULiège > Centre de recherches du cyclotron > > >]
Plenevaux, Alain mailto [Université de Liège - ULiège > Département de chimie (sciences) > Département de chimie (sciences) >]
Salvatori, Roberto mailto [Johns Hopkins University > Medicine > Division of Endocrinology, Diabetes and Metabolism > >]
Geenen, Vincent mailto [Université de Liège - ULiège > > Centre d'immunologie >]
Martens, Henri mailto [Université de Liège - ULiège > > Centre d'immunologie >]
Frontiers in Endocrinology
Frontiers Media S.A.
Art. 296
Yes (verified by ORBi)
[en] Somatotrope axis ; GH-releasing hormone ; Insulin-like growth factor 1 ; Thymus ; Spleen ; Developmental immunology
[en] A debate is still open about the precise control exerted by the somatotrope GH-releasing hormone (GHRH)/growth hormone (GH)/insulin-like growth factor 1 axis on the immune system. The objective of this study was to directly address this question through the use of Ghrh−/− mice that exhibit a severe deficiency of their somatotrope axis. After control backcross studies and normalization for the reduced global weight of transgenic mice, no difference in weight and cellularity of the thymus was observed in Ghrh−/− mice when compared with C57BL/6 wild-type (WT) control mice. Similarly, no significant change was observed in frequency and number of thymic T cell subsets. In the periphery, Ghrh−/− mice exhibited an increase in T cell proportion associated with a higher frequency of sjTREC and naïve T cells. However, all Ghrh−/− mice displayed an absolute and relative splenic atrophy, in parallel with a decrease in B cell percentage. GH supplementation of transgenic mice for 6 weeks induced a significant increase in their global as well as absolute and relative splenic weight. Interestingly, the classical thymus involution following dexamethasone administration was shown to recover in WT mice more quickly than in mutant mice. Altogether, these data show that the severe somatotrope deficiency of Ghrh−/− mice essentially impacts the spleen and B compart- ment of the adaptive immune system, while it only marginally affects thymic function and T cell development.
Giga-Infection, Immunity and Inflammation
Communauté française de Belgique - CfB (ARC Somasthym) ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds Léon Fredericq
Researchers ; Professionals ; Students

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