Capillary electrophoresis-mass spectrometry for targeted and untargeted analysis of the sub-5 kDa urine metabolome of patients with prostate or bladder cancer: a feasibility study
[en] Targeted and untargeted analyses of the sub-5 kDa urine metabolome of genitourinary cancer patients (prostate and/or bladder) were performed without chemical derivatization using capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS). For targeted analysis, endogenous levels of sarcosine and 5 other amino acid metabolites implicated in the progression of prostate cancer were quantified in four patients and in a pooled urine sample from healthy volunteers. An untargeted analysis (m/z 50 to 850) of patient urine was performed using the same CE-ESI-MS system identifying over 400 distinct molecular features per patient. All patient urine samples were collected at prostatectomy/cystectomy via catheter. Patient urine samples were filtered by centrifugation, with endogenous sarcosine enriched by solid-phase extraction, and the processed samples loaded onto CE-ESI-MS for analysis. Diagnostic information, digital pathological slides, and tissue samples were collected and stored in a comprehensive biobanking database. The introduction of urine sample collection into the surgery workflow was facile and is a promising strategy for addressing the translational research challenge of moving smoothly from “chromatogram to nomogram”.
Research Center/Unit :
CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège
Disciplines :
Human health sciences: Multidisciplinary, general & others
Author, co-author :
MacLennan, Matthew; University of British Columbia - UBC > Department of Chemistry
Kok, Miranda ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Soliman, Laiel; University of British Columbia - UBC > Department of Chemistry
So, Alan; Vancouver Prostate Centre
Hurtado-Coll, Antonio; Vancouver Prostate Centre
Chen, David; University of British Columbia - UBC > Department of Chemistry
Language :
English
Title :
Capillary electrophoresis-mass spectrometry for targeted and untargeted analysis of the sub-5 kDa urine metabolome of patients with prostate or bladder cancer: a feasibility study
Publication date :
01 February 2018
Journal title :
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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