Reference : Capillary electrophoresis-mass spectrometry for targeted and untargeted analysis of t...
Scientific journals : Article
Human health sciences : Multidisciplinary, general & others
http://hdl.handle.net/2268/224369
Capillary electrophoresis-mass spectrometry for targeted and untargeted analysis of the sub-5 kDa urine metabolome of patients with prostate or bladder cancer: a feasibility study
English
MacLennan, Matthew [University of British Columbia - UBC > Department of Chemistry > > >]
Kok, Miranda mailto [Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments >]
Soliman, Laiel [University of British Columbia - UBC > Department of Chemistry > > >]
So, Alan [Vancouver Prostate Centre > > > >]
Hurtado-Coll, Antonio [Vancouver Prostate Centre > > > >]
Chen, David [University of British Columbia - UBC > Department of Chemistry > > >]
1-Feb-2018
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Elsevier
1074-1075
79-85
Yes (verified by ORBi)
International
1570-0232
Netherlands
[en] metabolomics ; capillary electrophoresis ; prostate cancer ; bladder cancer ; urine ; amino acids
[en] Targeted and untargeted analyses of the sub-5 kDa urine metabolome of genitourinary cancer patients (prostate and/or bladder) were performed without chemical derivatization using capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS). For targeted analysis, endogenous levels of sarcosine and 5 other amino acid metabolites implicated in the progression of prostate cancer were quantified in four patients and in a pooled urine sample from healthy volunteers. An untargeted analysis (m/z 50 to 850) of patient urine was performed using the same CE-ESI-MS system identifying over 400 distinct molecular features per patient. All patient urine samples were collected at prostatectomy/cystectomy via catheter. Patient urine samples were filtered by centrifugation, with endogenous sarcosine enriched by solid-phase extraction, and the processed samples loaded onto CE-ESI-MS for analysis. Diagnostic information, digital pathological slides, and tissue samples were collected and stored in a comprehensive biobanking database. The introduction of urine sample collection into the surgery workflow was facile and is a promising strategy for addressing the translational research challenge of moving smoothly from “chromatogram to nomogram”.
Centre Interdisciplinaire de Recherche sur le Médicament - CIRM
http://hdl.handle.net/2268/224369
10.1016/j.jchromb.2018.01.007

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