GmPGIP3 enhanced resistance to both take-all and common root rot diseases in transgenic wheat

[en] Take-all (caused by the fungal pathogen Gaeumannomyces graminis var. tritici, Ggt) and common root rot (caused by Bipolaris sorokiniana) are devastating root diseases of wheat (Triticum aestivum L.). Development of resistant wheat cultivars has been a challenge since no resistant wheat accession is available. GmPGIP3, one member of polygalacturonase-inhibiting protein (PGIP) family in soybean (Glycine max), exhibited inhibition activity against fungal endopolygalacturonases (PGs) in vitro. In this study, the GmPGIP3 transgenic wheat plants were generated and used to assess the effectiveness of GmPGIP3 in protecting wheat from the infection of Ggt and B. sorokiniana. Four independent transgenic lines were identified by genomic PCR, Southern blot, and reverse transcription PCR (RT-PCR). The introduced GmPGIP3 was integrated into the genomes of these transgenic lines and could be expressed. The expressing GmPGIP3 protein in these transgenic wheat lines could inhibit the PGs produced by Ggt and B. sorokiniana. The disease response assessments postinoculation showed that the GmPGIP3-expressing transgenic wheat lines displayed significantly enhanced resistance to both take-all and common root rot diseases caused by the infection of Ggt and B. sorokiniana. These data suggested that GmPGIP3 is an attractive gene resource in improving resistance to both take-all and common root rot diseases in wheat.

Disciplines :

Biotechnology

Author, co-author :

Wang, Aiyun

Wei, Xuening

Rong, Wei ; Université de Liège - ULiège > Doct. sc. agro. & ingé. biol. (Paysage)

Dang, Liang

Du, Lipu

Qi, Lin

Xu, Huijun

Shao, Yanjun

Zhang, Zengyan

Language :

English

Title :

GmPGIP3 enhanced resistance to both take-all and common root rot diseases in transgenic wheat

Publication date :

2014

Journal title :

Functional and Integrative Genomics

ISSN :

1438-793X

eISSN :

1438-7948

Publisher :

Springer, Germany

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 28 May 2018

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