Article (Scientific journals)
Detection of plant protein adulterated in fluid milk using twodimensional gel electrophoresis combined with mass spectrometry
Yang, Jinhui; Zheng, Nan; Yang, Yongxin et al.
2018In Journal of Food Science and Technology, 55 (7), p. 2721-2728
Peer reviewed
 

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Keywords :
milk; adulteration; electrophoresis
Abstract :
[en] The illegal or unlabelled addition of plant protein in milk can cause serious anaphylaxis. For sustainable food security, it is therefore important to develop a methodology to detect non-milk protein in milk products. This research aims to differentiate milk adulterated with plant protein using two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry. According to the protein spots highlighted on the gel of adulterated milk,b-conglycinin and glycinin were detected in milk adulterated with soy protein, while legumin, vicilin, and convicilin indicated the addition of pea protein, and b-amylase and serpin marked wheat protein. These results suggest that a 2-DE-based protein profile is a useful method to identify milk adulterated with soy and pea protein, with a detection limit of 4% plant protein in the total protein.
Disciplines :
Food science
Author, co-author :
Yang, Jinhui;  Faculté Universitaire des Sciences Agronomiques de Gembloux - FUSAGx
Zheng, Nan;  Chinese academy of agricultural science
Yang, Yongxin;  Chinese academy for Agricultural Sciences
Wang, Jiaqi;  Chinese academy for agricultural sciences
Soyeurt, Hélène  ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Modélisation et développement
Language :
English
Title :
Detection of plant protein adulterated in fluid milk using twodimensional gel electrophoresis combined with mass spectrometry
Alternative titles :
[fr] Détectionde lait adultéré avec des protéines végétales par électrophorèse à 2 dimensions couplée à une spectrométrie de masse
Publication date :
July 2018
Journal title :
Journal of Food Science and Technology
ISSN :
0022-1155
eISSN :
0975-8402
Publisher :
Springer, Germany
Volume :
55
Issue :
7
Pages :
2721-2728
Peer reviewed :
Peer reviewed
Available on ORBi :
since 24 May 2018

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