[en] The illegal or unlabelled addition of plant protein in milk can cause serious anaphylaxis. For sustainable food security, it is therefore important to develop a methodology to detect non-milk protein in milk products. This research aims to differentiate milk adulterated with plant protein using two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry. According to the protein spots highlighted on the gel of adulterated milk,b-conglycinin and glycinin were detected in milk adulterated with soy protein, while legumin, vicilin, and convicilin indicated the addition of pea protein, and b-amylase and serpin marked wheat protein. These results suggest that a 2-DE-based protein profile is a useful method to identify milk adulterated with soy and pea protein, with a detection limit of 4% plant protein in the total protein.
Disciplines :
Food science
Author, co-author :
Yang, Jinhui; Faculté Universitaire des Sciences Agronomiques de Gembloux - FUSAGx
Zheng, Nan; Chinese academy of agricultural science
Yang, Yongxin; Chinese academy for Agricultural Sciences
Wang, Jiaqi; Chinese academy for agricultural sciences
Soyeurt, Hélène ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Modélisation et développement
Language :
English
Title :
Detection of plant protein adulterated in fluid milk using twodimensional gel electrophoresis combined with mass spectrometry
Alternative titles :
[fr] Détectionde lait adultéré avec des protéines végétales par électrophorèse à 2 dimensions couplée à une spectrométrie de masse
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