Reference : Development of nanoprobes for cancerous tissues imaging by Surface-Enhanced Raman Spe...
Scientific congresses and symposiums : Poster
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/223614
Development of nanoprobes for cancerous tissues imaging by Surface-Enhanced Raman Spectroscopy
English
Müller, Wendy mailto [Université de Liège - ULiège > Département de chimie > Laboratoire de Chimie Analytique Inorganique > >]
Verdin, Alexandre mailto [Université de Liège - ULiège > Département de chimie (sciences) > Département de chimie (sciences) >]
Malherbe, Cédric mailto [Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique >]
Eppe, Gauthier mailto [Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique >]
17-May-2018
No
No
International
Bioforum 2018
17 Mai 2018
Bioliège
Liège
Belgique
[en] Raman ; SERS ; imaging ; nanoprobe ; cancerous tissues
[en] Early detection and precise characterization of cancers is one of the major challenges in medicine. Consequently, there is a strong need to develop highly sensitive, specific and quick analytical techniques. Among the emerging methods allowing the analysis of cancerous samples, Surface-Enhanced Raman Spectroscopy (SERS), an amplified technique of vibrational spectroscopy based on Raman scattering, is particularly suitable. The signal enhancement in SERS, up to 10^12-fold in the presence of metallic nanoparticles near the Raman-active molecule, offers a better sensitivity than in “classic” Raman spectroscopy and thus allows the analysis of molecules in weaker concentrations. One of the advantages of the nanoparticles used in SERS is that they can be functionalized in order to specifically target a molecule of interest and therefore give information about the spatial location of biomarkers via SERS imaging. Our research aims at developing SERS nanoprobes functionalized with folic acid, complementary to the FRalpha receptor (folate receptor alpha), a membrane receptor overexpressed in many types of cancers. SERS imaging with these nanoprobes will then enable to spot cancerous areas within human cancerous tissues and to discriminate between healthy and cancerous tissues. The preliminary results of our study show that nanoprobes can bind to tissues and more particularly at the level of membrane structures and that they can be detected via SERS imaging. SERS imaging also allows to observe a signal accumulation in presumed cancerous areas, in correlation with the histological H&E analysis. Finally, SERS imaging of healthy and cancerous tissues seems to indicate higher signal intensity within cancerous tissues than within healthy tissues.
Laboratoire de Chimie Analytique Inorganique
Researchers ; Professionals
http://hdl.handle.net/2268/223614

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