The peculiar NPQ regulation in the stramenopile Phaeomonas sp. challenges the xanthophyll cycle dogma

[en] In changing light conditions, photosynthetic organisms develop different strategies to maintain a fine balance between light harvesting, photochemistry, and photoprotection. One of the most widespread photoprotective mechanisms consists in the dissipation of excess light energy in the form of heat in the photosystem II antenna, which participates to the Non Photochemical Quenching (NPQ) of chlorophyll fluorescence. It is tightly related to the reversible epoxidation of xanthophyll pigments, catalyzed by the two enzymes, the violaxanthin deepoxidase and the zeaxanthin epoxidase. In Phaeomonas sp. (Pinguiophyte, Stramenopiles), we show that the regulation of the heat dissipation process is different from that of the green lineage: the NPQ is strictly proportional to the amount of the xanthophyll pigment zeaxanthin and the xanthophyll cycle enzymes are differently regulated. The violaxanthin deepoxidase is already active in the dark, because of a low luminal pH, and the zeaxanthin epoxidase shows a maximal activity under moderate light conditions, being almost inactive in the dark and under high light. This light-dependency mirrors the one of NPQ: Phaeomonas sp. displays a large NPQ in the dark as well as under high light, which recovers under moderate light. Our results pinpoint zeaxanthin epoxidase activity as the prime regulator of NPQ in Phaeomonas sp. and therefore challenge the deepoxidase-regulated xanthophyll cycle dogma. © 2018 Elsevier B.V.

Disciplines :

Biochemistry, biophysics & molecular biology
Phytobiology (plant sciences, forestry, mycology...)

Author, co-author :

Berne, Nicolas ; Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues

Fabryova, T.; Genetics and Physiology of microalgae, PhytoSYSTEMS/InBioS, Université de Liège, Liège, Belgium

Istaz, B.; Genetics and Physiology of microalgae, PhytoSYSTEMS/InBioS, Université de Liège, Liège, Belgium

Cardol, Pierre ; Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues

Bailleul, Benjamin ; Genetics and Physiology of microalgae, PhytoSYSTEMS/InBioS, Université de Liège, Liège, Belgium, UMR7141, Institut de Biologie Physico-Chimique (IBPC), Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie, 13 rue Pierre et Marie Curie F-75005 Paris, France

Language :

English

Title :

The peculiar NPQ regulation in the stramenopile Phaeomonas sp. challenges the xanthophyll cycle dogma

Publication date :

2018

Journal title :

Biochimica et Biophysica Acta. Bioenergetics

ISSN :

0005-2728

eISSN :

1879-2650

Publisher :

Elsevier B.V.

Volume :

1859

Issue :

Pages :

491-500

Peer reviewed :

Peer Reviewed verified by ORBi

European Projects :

H2020 - 682580 - BEAL - Bioenergetics in microalgae : regulation modes of mitochondrial respiration, photosynthesis, and fermentative pathways, and their interactions in secondary algae

Name of the research project :

FNRS (CDR J.0032, CDR J.0079 and Incentive Grant for Scientific Research F.4520); EU grant agreement no 682580; EU grant agreement no 715579

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
CER - Conseil Européen de la Recherche [BE]
UE - Union Européenne [BE]
CE - Commission Européenne [BE]

Available on ORBi :

since 16 May 2018

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