[en] Aim
: This study aimed to investigate the protective effect of
Rosmarinus officinalis
(L.) essential oil on rooster sperm
motility during 4°C short-term storage.
Materials and Methods
:
R. officinalis
essential oil was analyzed using gas chromato
graphy coupled to mass spectrometry
to identify the active components. 10 of 45-week-old Hubbard commercial broilers were subjected to biweekly semen
collections during 3
weeks. At each collection, sperm was pooled and divided into four aliquots and then diluted with Tris
extender supplemented with 870, 87, or 8.7
μg/ml of
R. officinalis
essential oil, identified as treatments R, R5, and R10,
respectively. Tris-based extender without any supplementation was considered as a control group. Diluted sperm was then
stored at 4°C in the refrigerator and analyzed at 0, 6, 24, and 48
h using a computer-assisted
sperm analyzer. Different semen
parameters were measured including total motility, progressive motility, gametes velocities (straight line velocity [VSL],
curvilinear velocity [VCL], and average path velocity [VAP]), amplitude of the lateral head displacement [ALH], and beat-
cross frequency [BCF].
Results
: The phytochemical analysis of
R. officinalis
essential oil revealed the presence of 25 active components
including
seven major molecules: Camphor (18.88%), camphene (5.17%), 1,8-cineole (7.85%), β-thujene (13.66%), α-thujene (4.87%),
chrysanthenone (12.05%), and β-cubenene (7.97%). The results showed a beneficial effect of
R. officinalis
essential oil on
sperm cells motility, particularly when using the lowest concentrations, 8.7 and 87
μg/ml.
Progressive motility and gametes
velocities (VCL, VSL, and VAP), materializing the quality of gametes motility, showed highly statistically significant
values (p<0.01) in 8.7 and 87
μg/ml treatments, especially from 6
h of storage at 4°C. Conversely, the highest concentration
(870
μg/ml) showed harmful effects with a total spermicidal activity after 24
h of storage.
Conclusion
: The current results revealed the positive impact of
R. officinalis
essential oil on rooster sperm at 4°C short-term
storage probably through
fighting against oxidative stress and cold shock damages.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Touazi, Leghel; Associated Laboratory in Marine and Aquaculture Ecosystems, Faculty of Nature and Life Sciences, University of Bej
Aberkane, B.; 3. Department of Biology, Faculty of Nature and Life Sciences and Earth Sciences. University of Bouira, Alge
Bellik, Y.; 4. Department of Biology, Faculty of Nature and Life Sciences, University of El Bachir el Ibrahimi, Bordj Bou Arreridj, 34000, Alge
Moula, Nassim ; Université de Liège - ULiège > Scientifiques attachés au Doyen (F MV)
Iguer-Ouada, M.; Associated Laboratory in Marine and Aquaculture Ecosystems, Faculty of Nature and Life Sciences, University of Bejaia. Algerie
Language :
English
Title :
Effect of the essential oil of Rosmarinus officinalis (L.) on rooster sperm motility during 4°C short-term storage
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