Transcriptional effects of phospholipid fatty acid profile on rainbow trout liver cells exposed to methylmercury

[en] Lipids, and their constitutive fatty acids, are key nutrients for fish health as they provide energy, maintain cell structure, are precursors of signalling molecules and act as nuclear receptor ligands. These specific roles may be of crucial importance in a context of exposure to pollutants. We recently showed that the fatty acid profile of rainbow trout liver cell phospholipids modulates sensitivity to an acute methylmercury challenge. In order to investigate mechanisms of effects, we herein tested whether specific polyunsaturated fatty acids may protect cells from methylmercury through decreasing intracellular mercury accumulation and/or enhancing cellular defences (e.g. via modulation of gene expression patterns). We also investigated the inverse relationship and assessed the impact of methylmercury on cellular fatty acid metabolism. To do so, the fatty acid composition of rainbow trout liver cell phospholipids was first modified by incubating them in a medium enriched in a specific polyunsaturated fatty acid (PUFA) from either the n-3 family (alpha-linolenic acid, ALA; eicosapentaenoic acid, EPA) or the n-6 family (linoleic acid, LA; arachidonic acid, AA). Cells were then exposed to methylmercury (0.15 or 0.50 μM) for 24 h and sampled thereafter for assessing phospholipid fatty acid profile, intracellular total mercury burden, and expression pattern of genes involved in fatty acid metabolism, synthesis of polyunsaturated fatty acid-derived signalling molecules and stress response. We observed that cells incorporated the given polyunsaturated fatty acid and some biotransformation products in their phospholipids. MeHg had few impacts on this cellular phospholipid composition. None of the PUFA enrichments affected the cellular mercury burden, suggesting that the previously observed cytoprotection conferred by ALA and EPA was not linked to a global decrease in cellular accumulation of mercury. Fatty acid enrichments and methylmercury exposure both modulated gene expression patterns. Genes involved in the synthesis of polyunsaturated fatty acid-derived signalling molecules, in stress response and the orphan cytochrome P450 20A1 were identified as possible sites of interaction between fatty acids and methylmercury in rainbow trout liver cells.

Research Center/Unit :

FOCUS - Freshwater and OCeanic science Unit of reSearch - ULiège

Disciplines :

Aquatic sciences & oceanology
Environmental sciences & ecology

Author, co-author :

Ferain, Aline

Bonnineau, Chloé

Neefs, Inneke

Das, Krishna ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Océanographie biologique

Larondelle, Yvan

Rees, Jean-François

Debier, Cathy

Lemaire, Benjamin

Language :

English

Title :

Transcriptional effects of phospholipid fatty acid profile on rainbow trout liver cells exposed to methylmercury

Publication date :

2018

Journal title :

Aquatic Toxicology

ISSN :

0166-445X

eISSN :

1879-1514

Publisher :

Elsevier, Netherlands

Volume :

199

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://www.sciencedirect.com/science/article/pii/S0166445X18302819

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique

Available on ORBi :

since 27 March 2018

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