[en] The proposal that the respiratory complexes can associate with each other in larger structures named super- complexes (SC) is generally accepted. In the last decades most of the data about this association came from studies in yeasts, mammals and plants, and information is scarce in other lineages. Here we studied the su- pramolecular association of the F1FO-ATP synthase (complex V) and the respiratory complexes I, III and IV of the colorless alga Polytomella sp. with an approach that involves solubilization using mild detergents, n-dodecyl-β-D- maltoside (DDM) or digitonin, followed by separation of native protein complexes by electrophoresis (BN- PAGE), after which we identified oligomeric forms of complex V (mainly V2 and V4) and different respiratory supercomplexes (I/IV6, I/III4, I/IV). In addition, purification/reconstitution of the supercomplexes by anion exchange chromatography was also performed. The data show that these complexes have the ability to strongly associate with each other and form DDM-stable macromolecular structures. The stable V4 ATPase oligomer was observed by electron-microscopy and the association of the respiratory complexes in the so-called “respirasome” was able to perform in-vitro oxygen consumption.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Miranda Astudillo, Héctor Vicente ; Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues
Colina-Tenorio, Lilia
Jiménez-Suárez, Alejandra
Vázquez-Acevedo, Miriam
Salin, Bénédicte
Giraud, Marie-France
Remacle, Claire ; Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues
Cardol, Pierre ; Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues
González-Halphen, Diego
Language :
English
Title :
Oxidative phosphorylation supercomplexes and respirasome reconstitution of the colorless alga Polytomella sp
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