Laboratory Findings Suggesting an Association Between BoHV-4 and Bovine Abortions in Southern Belgium

Belgium; Bovine herpesvirus 4; Aborted Fetus; Abortion, Veterinary; Animals; Cattle; Cattle Diseases; Female; Herpesviridae Infections; Herpesvirus 4, Bovine; Parity; Prevalence; Seasons; Seroconversion; Seroepidemiologic Studies; Tumor Virus Infections

Abstract :

[en] Abortions cause heavy economic losses for the bovine sector. The use of a standardized panel of analyses covering a large spectrum of pathogens responsible of abortion in cattle allowed demonstrating the direct involvement of at least one pathogen in 57% of analysed abortions in the southern part of Belgium. This result suggests a margin of improvement in the diagnostic efficacy. In order to evaluate the interest to broaden the list of pathogens included in the panel of analyses, the implication of bovine herpesvirus 4 (BoHV-4) in abortion was assessed by two different studies. In the first study, coupled serology was performed after abortion on 714 dams to identify specific seroconversion against BoHV-4. The overall seroconversion in cows was 19.5%, with a higher frequency in primiparous compared to multiparous females. In addition, the type of breed (beef cattle) and the time period from the fourth quarter 2008 until the last quarter 2009 were significantly related to the seroconversion of cows. The second study investigated the virus ability to infect the foetus. In this study, 368 cases of bovine abortions were specifically tested for BoHV-4, using PCR on foetus tissues and ELISA on dam and foetus sera. The results showed a maternal seroprevalence of 64.7%, a foetal seroprevalence of 0.8% and a PCR prevalence in foetuses of 1.1%, demonstrating the ability of BoHV-4 to infect the foetus. © 2016 Blackwell Verlag GmbH

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Delooz, L.; Département Santé Animale, Association Régionale de Santé et d'Identification Animales – ASBL, Ciney, Belgium, Research Unit of Epidemiology and Risk Analysis applied to Veterinary Sciences (UREAR-ULg), Centre for Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liege, Liege, Belgium

Czaplicki, G.; Département Santé Animale, Association Régionale de Santé et d'Identification Animales – ASBL, Ciney, Belgium

Houtain, J. Y.; Département Santé Animale, Association Régionale de Santé et d'Identification Animales – ASBL, Ciney, Belgium

Dal Pozzo, Fabiana ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Epidémiologie et analyse des risques appl. aux sc. vétér.

Saegerman, Claude ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Epidémiologie et analyse des risques appl. aux sc. vétér.

Language :

English

Title :

Laboratory Findings Suggesting an Association Between BoHV-4 and Bovine Abortions in Southern Belgium

Publication date :

2017

Journal title :

Transboundary and Emerging Diseases

ISSN :

1865-1674

eISSN :

1865-1682

Publisher :

Blackwell Publishing Ltd

Volume :

Issue :

Pages :

1100-1109

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 12 March 2018

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Bibliography

Bailey, N. M., M. P. Cunningham, and C. B. Kimber, 1967: The indirect fluorescent antibody technique applied to dried blood, for use as a screening test in the diagnosis of human trypanosomiasis in Africa. Trans. R. Soc. Trop. Med. Hyg. 61, 696–700.
Czaplicki, G., and E. Thiry, 1998: An association exists between bovine herpesvirus-4 seropositivity and abortion in cows. Prev. Vet. Med. 33, 235–240.
Dean, A. D., J. A. Dean, A. K. Burton, and R. C. Dicker, 1990: Epi info, Version 5: A Word Processing Database and Statistical Program for Epidemiology on Micro-Computers, pp. 252–254. USD Inc., Stone Mountain, Georgia.
Deim, Z., L. Szeredi, V. Tompo, and L. Egyed, 2006: Detection of bovine herpesvirus 4 in aborted bovine placentas. Microbial. Path. 41, 144–148.
Deim, Z., L. Szeredi, and L. Egyed, 2007: Detection of bovine herpesvirus 4 DNA in aborted bovine foetuses. Can. J. Vet. Res. 71, 226–229.
Donofrio, G., L. Ravanetti, S. Cavirani, S. Herath, A. Capocefalo, and I. M. Sheldon, 2008: Bacterial infection of endometrial stromal cells influences bovine herpesvirus 4 immediate early gene activation: a new insight into bacterial and viral interaction for uterine disease. Reproduction 136, 361–366.
Edwards, S., and R. H. Newman, 1985: Detection of antibodies to bovid herpesvirus 4 by ELISA. Vet. Microbiol. 10, 149–154.
Egyed, L., G. Sassi, J. Tibold, I. Madi, and O. Szenci, 2011: Symptomless intrauterine transmission of bovine herpesvirus 4 to bovine foetuses. Microb. Pathog. 50, 322–325.
Fitton, J., J. Beenham, and S. Edwards, 1990: Bovid herpesvirus-4 antibody in cattle in Great Britain. Vet. Rec. 126, 173.
Kendrick, J. W., B. I. Osburn, and N. Kronlund, 1976: Pathogenicity studies on a bovine herpesvirus. Theriogenology 6, 447–462.
Kirkbride, C. A., 1992: Viral agents and associated lesions detected in a 10-year study of bovine abortions and stillbirths. J. Vet. Diagn. Invest. 4, 374–379.
Leboeuf, C., 2013: Six pour-cent d'avortements positifs en PCR pour le BHV4 dans la manche. Point Vét. 332, 60–64.
Monge, A., L. Elvira, J. V. Gonzalez, S. Astiz, and G. J. Wellenberg, 2006: Bovine herpesvirus 4-associated postpartum metritis in a Spanish dairy herd. Res. Vet. Sci. 80, 120–125.
Naeem, K., S. M. Goyal, and R. E. Werdin, 1989: Prevalence of bovid herpesvirus-4 and its antibody in cattle in Minnesota. Am. J. Vet. Res. 50, 1931–1935.
Nak, Y., S. B. Dagalp, C. Cetin, D. Nak, F. Alkan, and E. Borum, 2011: Course and severity of postpartum metritis cases following antibiotic and PGF2α administration in postpartum metritis cows infected with BoHV-4. Transbound Emerg. Dis. 58, 31–36.
Petrie, A., and P. Watson, 2006: Additional Techniques in Statistics for Veterinary and Animal Science, 2nd edn. vol 14. pp. 191–211. Blackwell Science, Oxford.
Preux, P. M., P. Odermatt, A. Perna, B. Marin, and A. Vergnenègre, 2005: Qu'est-ce qu'une régression logistique ? Rev. Mal. Respir. 22, 159–162.
Reed, D. E., T. J. Langpap, and M. E. Bergeland, 1979: Bovine abortion associated with mixed Movar 33/63 type herpesvirus and bovine viral diarrhoea virus infection. Cornell. Vet. 69, 54–66.
Sheldon, I. M., J. Cronin, L. Goetze, G. Donofrio, and H. J. Schuberth, 2009: Defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle. Biol. Reprod. 81, 1025–1032.
StataCorp, 2012: Stata Statistical Software: Stata/SE Acad. 12. College Station, TX. 333 StataCorp LP.
Thiry, E., 2007: Maladies Virales Des Ruminants. 2nd edn. Éditions du Point Vétérinaire, Rueil-Malmaison 301 p.
Treilles, M., F. Benoit, V. Carpinshi, M. Leborgne, and B. Blanchard, 2013: Progrès dans la mise en évidence du BoHV-4 en routine. Le Point Vétérinaire. 332, 66–68.
Van Malderen, G., E. Van Opdenbosch, and G. Wellemans, 1987: Bovien herpes virus 1 en 4: een sero-epidemiologisch onderzoek van de Belgische Rundveestapel. Vlaams Diergeneesk. Tijdschr. 56, 364–371.
Van Opdenbosch, E., G. Wellemans, H. Antoine, A. Broers, and G. Charlier, 1984: Chronic metritis, associated with various symptoms: an immunodepressive herpes virus (LVR 140) as etiological agent?. Vlaams Diergeneesk. Tijdschr. 53, 21–30.
Van Opdenbosch, E., G. Wellemans, H. Theys, and I. Verhees, 1986: Occurence of subclinical virus infection in veal calves and their influence on weight gain. Vlaams Diergeneesk. Tijdschr. 50, 17–20.
Verna, A. E., J. M. Manrique, S. E. Perez, M. R. Leunda, S. B. Pereyra, L. R. Jones, and A. C. Odeon, 2012: Genomic analysis of bovine herpesvirus type 4 from Argentina: High genetic variability and novel phylogenic groups. Vet. Microbiol. 160, 1–8.
Wellemans, G., 1984: Symptomatologie variée apparaîssant lors des métrites chroniques associées à un virus herpès chez les bovins. Ann. Méd. Vét. 128, 614–615.
Wellemans, G., and E. Van Opdenbosch, 1987: Transmission horizontale du virus BHV-4 à des génisses. Ann. Méd. Vét. 121, 487–491.
Wellemans, G., and E. Van Opdenbosch, 1989: Association entre l'infection par le BoHV4 et l'avortement chez la vache. Ann. Méd. Vét. 133, 347–350.
Wellemans, G., E. Van Opdenbosch, and M. Mammerickx, 1986: Experimental inoculation of bovine herpesvirus 4 (strain LVR 140) in pregnant and nonpregnant cows. Ann. Rech. Vet. 17, 89–94.
Wellenberg, G. J., E. M. A. Van Rooij, J. Maissan, and J. T. Van Oirschot, 1999: Evaluation of newly developed immunoperoxidase monolayer assays for detection of antibodies against bovine herpesvirus 4. Clin. Diagn. Lab. Immunol. 6, 447–451.
Wellenberg, G. J., E. R. A. M. Verstraten, S. Belak, S. B. E. Verschuren, F. A. M. Rijsewijk, R. Peshev, and J. T. Van Oirschot, 2001: Detection of bovine herpesvirus 4 glycoprotein B and thymidine kinase DNA by PCR assays in bovine milk. J. Virol. Methods 97, 101–112.