Improved aptamers for the diagnosis and potential treatment of HER2-positive cancer

[en] Aptamers provide a potential source of alternative targeting molecules for existing antibody diagnostics and therapeutics. In this work, we selected novel DNA aptamers targeting the HER2 receptor by an adherent whole-cell SELEX approach. Individual aptamers were identified by next generation sequencing and bioinformatics analysis. Two aptamers, HeA2_1 and HeA2_3, were shown to bind the HER2 protein with affinities in the nanomolar range. In addition, both aptamers were able to bind with high specificity to HER2-overexpressing cells and HER2-positive tumor tissue samples. Furthermore, we demonstrated that aptamer HeA2_3 is being internalized into cancer cells and has an inhibitory effect on cancer cell growth and viability. In the end, we selected novel DNA aptamers with great potential for the diagnosis and possible treatment of HER2-positive cancer. © 2016 by the authors; licensee MDPI, Basel, Switzerland.

Disciplines :

Biotechnology

Author, co-author :

Gijs, M.; Radiobiology Unit, Belgian Nuclear Research Centre (SCK CEN), Mol, Belgium, Cyclotron Research Centre, University of Liège, Liège, Belgium

Penner, G.; NeoVentures Biotechnology Inc, London, ON, Canada

Blackler, G. B.; NeoVentures Biotechnology Inc, London, ON, Canada

Impens, N. R. E. N.; Radiobiology Unit, Belgian Nuclear Research Centre (SCK CEN), Mol, Belgium

Baatout, S.; Radiobiology Unit, Belgian Nuclear Research Centre (SCK CEN), Mol, Belgium

Luxen, André ; Université de Liège - ULiège > Centre de Recherches du Cyclotron

Aerts, A. M.; Radiobiology Unit, Belgian Nuclear Research Centre (SCK CEN), Mol, Belgium

Title :

Improved aptamers for the diagnosis and potential treatment of HER2-positive cancer

Publication date :

2016

Journal title :

Pharmaceuticals

eISSN :

1424-8247

Publisher :

MDPI AG

Volume :

Issue :

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 20 February 2018

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