[en] Poly(N-methylvinylamines) with secondary amines can form complexes with plasmid DNA (pDNA) and provide transfection efficiency in HeLa cells in the same order as linear polyethyleneimine but with higher cell viability. Chemical modifications of poly(N-methylvinylamine) backbones are performed to further improve transfection efficiency while maintaining low degree of cytotoxicity. In a first type of polymer, primary amino groups are incorporated via a copolymerization strategy. In a second one, primary amino and imidazole groups are incorporated also via a copolymerization strategy. In a third one, secondary amino groups are substituted with methylguanidine functions through a postpolymerization reaction. Thus, novel polymers of various molecular masses are synthesized, characterized, and their interaction with pDNA studied. Then, their transfection efficiency and cytotoxicity are tested in HeLa cells. Two polymethylvinylamine-based copolymers, one containing 20% of imidazole moieties and another one composed of 12% of guanidinyl units allow remarkable transfection efficiency of HeLa, pulmonary (16HBE), skeletal muscle (C2C12), and dendritic (DC2.4) cells. Overall, this work thus identifies new promising DNA carriers and chemical modifications that improve the transfection efficiency while maintaining low degree of cytotoxicity.
Research center :
Center for Education and Research on Macromolecules (CERM) CESAM - Complex and Entangled Systems from Atoms to Materials - ULiège
Disciplines :
Materials science & engineering Chemistry
Author, co-author :
Dréan, Mathilde; University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM) > Sorbonne University, Institut Parisien de Chimie Moléculaire (IPCM), Paris, France
Debuigne, Antoine ; University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM)
Jérôme, Christine ; University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM)
Goncalves, Cristine; Centre Biophysique Moléculaire, CNRS, Orléans, France
Midoux, Patrick; Centre Biophysique Moléculaire, CNRS, Orléans, France
Rieger, Jutta; Sorbonne University, Institut Parisien de Chimie Moléculaire (IPCM), Paris, France
Guégan, Philippe; Sorbonne University, Institut Parisien de Chimie Moléculaire (IPCM), Paris, France
Language :
English
Title :
Poly(N-methylvinylamine)-based copolymers for improved gene transfection
Publication date :
April 2018
Journal title :
Macromolecular Bioscience
ISSN :
1616-5187
eISSN :
1616-5195
Publisher :
John Wiley & Sons, United Kingdom
Volume :
18
Issue :
4
Pages :
1700353
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
The International Doctoral School IDS-Funmat BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
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