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High-speed analysis of dansyl derivatives of polyamines
Bontemps, J.; Etienne, A.; Kadri, Meihdi et al.
1984In Chromatographia, 18 (9), p. 525-527
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Keywords :
Column liquid chromatography; Dansyl derivatives; Peak compression; Polyamines
Abstract :
[en] Analytical conditions for the high-speed, reversed-phase, liquid chromatographic of a diamine (putrescine) and polyamines (spermine and spermidine) were determined. Various elution modes were employed using the same mobile phase constituents: 20mM sodium heptane sulfonate and 20mM acetic acid as solvent A and, pure acetonitrile as solvent B. Samples were derivatized with dansyl chloride before injection. Under isocratic conditions, the separation of the three polyamines was achieved in 7 min. The use of a linear elution gradient led to the same analytical time but with a better resolution of the putrescine peak from non-polyamine frontal peaks. For these measurements, the sample size was 5μl. This volume was increased to 20μl and the use of a steep gradient combined with the peak-compression technique allowed a fast analysis in 2-3 minutes, which may be compared with a 30 min run time necessary when a conventional column is used. © 1984 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Bontemps, J.;  Laboratoires de Biochimie et de Pédiatrie, Université de Liège, Place Delcour, 17, Liège, 4020, Belgium
Etienne, A.;  Laboratoires de Biochimie et de Pédiatrie, Université de Liège, Place Delcour, 17, Liège, 4020, Belgium
Kadri, Meihdi ;  Centre Hospitalier Universitaire de Liège - CHU > Secteur accueil patient
van Cutsem, J.-L.;  Laboratoires de Biochimie et de Pédiatrie, Université de Liège, Place Delcour, 17, Liège, 4020, Belgium
Dandrifosse, Guy ;  Université de Liège - ULiège > Relations académiques et scientifiques (Médecine)
Forget, P.-P.;  Laboratoires de Biochimie et de Pédiatrie, Université de Liège, Place Delcour, 17, Liège, 4020, Belgium
Language :
English
Title :
High-speed analysis of dansyl derivatives of polyamines
Publication date :
1984
Journal title :
Chromatographia
ISSN :
0009-5893
eISSN :
1612-1112
Publisher :
Elsevier, Netherlands
Volume :
18
Issue :
9
Pages :
525-527
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 12 February 2018

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