Effect of salinity stress on finger millet (Eleusine coracana (L.) Gaertn): Histochemical and morphological analysis of coleoptile and coleorhizae - 2016
[en] The current study deals with the impact of salinity stress on growth and development of finger millet genotype ‘CO(Ra)-14’. The seeds were germinated on Murashige and Skoog solid medium supplemented with various concentrations of sodium chloride (NaCl) (0, 50, 100, 150 or 200 mM) for 15 days. Several physiological indices, including relative growth rate, electrolyte leakage, proline and chlorophyll (a, b and a + b) content, cell viability, hydrogen peroxide and caspase-like activity were measured to analyze the physiological and biochemical characteristics of saline stress. In addition, we also analyzed saline stress induced histochemical changes and level of lignin in the coleoptile and coleorhizae tissues. Relative growth of leaves and shoots of finger millet genotype ‘CO(Ra)-14’ exposed to 150 mM NaCl concentration was decreased while root length was increased. In addition, there was a significant increase in the levels of electrolyte leakage, contents of proline and hydrogen peroxide, and caspase-like activity from 0 to 200 mM NaCl. Chlorophyll and relative water contents were significantly reduced with decreased leaf succulence under increasing salinity. The microscopic observations of coleoptile and coleorhizae cross sections revealed considerable variations viz., length of epidermal cells, surface area and width of the cells with increasing salinity. Saline treated tissues were severely damaged and were intensely stained with phloroglucinol (suggesting the accumulation of cell bound phenolic compounds). Overall finger millet genotype ‘CO(Ra)-14’ showed a diverse response to salinity stress starting from seed germination to growth, and above 100 mM NaCl salinity is toxic to finger millet genotype ‘CO(Ra)-14’.
Disciplines :
Biotechnologie
Auteur, co-auteur :
Satish, Lakkakula
Periyasamy, Rathinapriya
Rency
Stanislaus, Antony Ceasar ; Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Meesala
Subramani
Ramakrishnan, Rameshkumar
Manikandan, Ramesh
Langue du document :
Anglais
Titre :
Effect of salinity stress on finger millet (Eleusine coracana (L.) Gaertn): Histochemical and morphological analysis of coleoptile and coleorhizae
Date de publication/diffusion :
avril 2016
Titre du périodique :
Flora
ISSN :
0367-2530
eISSN :
1618-0585
Maison d'édition :
Elsevier, Pays-Bas
Volume/Tome :
222
Pagination :
111-120
Peer reviewed :
Peer reviewed vérifié par ORBi
Organisme subsidiant :
University Grants Commission, New Delhi, India (UGC order no. F.4-1/2006 (BSR)/7-326/2011-BSR).
Ashraf M., Bashir A. Salt stress induced changes in some organic metabolites and ionic relations in nodules and other plant parts of two crop legumes differing in salt tolerance. Flora 2003, 198:486-498.
Ashraf M., Harris P.J.C. Potential biochemical indicators of salinity tolerance in plants. Plant Sci. 2004, 166:3-16.
Ashraf M.A., Ashraf M., Shahbaz M. Growth stage-based modulation in antioxidant defense system and proline accumulation in two hexaploid wheat (Triticum aestivum L.) cultivars differing in salinity tolerance. Flora 2012, 207:388-397.
Ashraf M. Some important physiological selection criteria for salt tolerance in plants. Flora 2004, 199:361-376.
Bates L.S., Waldren R.P., Teare I.D. Rapid determination of free proline for water-stress studies. Plant Soil 1973, 39:205-207.
Bray E.A., Bailey-Serres J., Weretilnyk E. Responses to abiotic stresses. Biochemistry and Molecular Biology of Plants 2000, 1158-1203. American Society of Plant Physiologists, Rockville. B. Buchanan, W. Gruissem, R. Jones (Eds.).
Ceasar S.A., Ignacimuthu S. Efficient somatic embryogenesis and plant regeneration from shoot apex explants of different Indian genotypes of finger millet (Eleusine coracana (L.) Gaertn.). In Vitro Cell. Dev. Biol. Plant 2008, 44:427-435.
Ceasar S.A., Ignacimuthu S. Genetic engineering of millets: current status and future prospects. Biotechnol. Lett. 2009, 31:779-788.
Ceasar S.A., Ignacimuthu S. Agrobacterium-mediated transformation of finger millet (Eleusine coracana (L.) Gaertn.) using shoot apex explants. Plant Cell Rep. 2011, 30:1759-1770.
Cordoba A., Seffino L.G., Moreno H., Arias C., Grunberg K., Zenoff A. Characterization of the effect of high salinity on roots of Chloris gayana Kunth: carbohydrate and lipid accumulation and growth. Grass Forage Sci. 2001, 56:162-168.
Cramer G.R., Bowman D.C. Kinetics of maize leaf elongation I: increased yield threshold limits short-term, steady-state elongation rates after exposure to salinity. J. Exp. Bot. 1991, 42:1417-1426.
Dong H., Kong X., Luo Z., Li W., Xin C. Unequal salt distribution in the root zone increases growth and yield of cotton. Eur. J. Agron. 2010, 33:285-292.
Dorias M., Papadopoulos A.P., Gosselin A. Greenhouse tomato fruit quality. Hort. Rev. 2001, 26:239-319.
El-Keblawy A., Al-Rawai A. Effects of seed maturation time and dry storage on light and temperature requirements during germination in invasive Prosopis juliflora. Flora 2006, 201:135-143.
Erfanzadeh R., Hendrickx F., Maelfait J., Hoffmann M. The effect of successional stage and salinity on the vertical distribution of seeds in salt marsh soils. Flora 2010, 205:442-448.
Flowers T.J. Improving crop salt tolerance. J. Exp. Bot. 2004, 55:307-319.
Garg A.K., Kim J.K., Owens T.G., Ranwala A.P., Choi Y.D., Kochian L.V., Wu R.J. Trehalose accumulation in rice plants confers high tolerance levels to different abiotic stresses. Proc. Natl. Acad. Sci. U. S. A 2002, 99:15898-15903.
Gorai M., Ennajeh M., Khemira H., Neffati M. Combined effect of NaCl-salinity and hypoxia on growth, photosynthesis, water relations and solute accumulation in Phragmites australis plants. Flora 2010, 205:462-470.
Hameed M., Ashraf M. Physiological and biochemical adaptations of Cynodon dactylon (L.) Pers. from the Salt Range (Pakistan) to salinity stress. Flora 2008, 203:683-694.
Hema R., Vemanna R.S., Sreeramulu S., Reddy C.P., Senthil-Kumar M., Udayakumar M. Stable expression of mtlD gene imparts multiple stress tolerance in finger millet. PLoS One 2014, 9(6):e99110. 10.1371/journal.pone.0099110.
Hussain T., Koyro H.W., Huchzermeyer B., Khan M.A. Eco-physiological adaptations of Panicum antidotale to hyperosmotic salinity: water and ion relations and anti-oxidant feedback. Flora 2015, 212:30-37.
Kishore P.B.K., Sangam S., Amrutha R.N., Laxmi P.S., Naidu K.R., Rao K.R.S.S., Rao S., Reddy K.J., Theriappan P., Sreenivasulu N. Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance. Curr. Sci. 2005, 88:424-438.
Krishnamurthy L., Upadhyaya H.D., Purushothaman R., Gowda C.L.L., Kashiwagi J., Dwivedi S.L., Singh S., Vadez V. The extent of variation in salinity tolerance of the mini core collection of finger millet (Eleusine coracana L. Gaertn.) germplasm. Plant Sci. 2014, 227:51-59.
Lichtenthaler H.K. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol. 1987, 148:350-382.
Lutts S., Almansouri M., Kinet J. Salinity and water stress have contrasting effects on the relationship between growth and cell viability during and after stress exposure in durum wheat callus. Plant Sci. 2004, 167:9-18.
Manchanda G., Garg N. Salinity and its effect on the functional biology of legumes. Acta Physiol. Plant. 2008, 30:595-618.
Monteiro C.C., Carvalho R.F., Gratao P.L., Carvalho G., Tezotto T., Medici L.O., Peres L.E.P., Azevedo R.A. Biochemical responses of the ethylene-insensitive Never ripe tomato mutant subjected to cadmium and sodium stresses. Environ. Exp. Bot. 2011, 71:306-320.
Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 1962, 15:473-497.
Naidoo G., Somaru R., Achar P. Morphological and physiological responses of the halophyte Odyssea paucinervis (Staph) (Poaceae), to salinity. Flora 2008, 203:437-447.
Parida A.K., Das A.B. Salt tolerance and salinity effects on plants. Ecotoxicol. Env. Saf. 2005, 60:324-349.
Pascale S.D., Maggio A., Barbieri G. Soil salinization affects growth, yield and mineral composition of cauliflower and broccoli. Eur. J. Agron. 2005, 23:254-264.
Perez-Lopez U., Robredo A., Miranda-Apodaca J., Lacuesta M., Munoz-Rueda A., Mena-Petite A. Carbon dioxide enrichment moderates salinity-induced effects on nitrogen acquisition and assimilation and their impact on growth in barley plants. Environ. Exp. Bot. 2013, 87:148-158.
Poborilova Z., Opatrilova R., Babula P. Toxicity of aluminium oxide nanoparticles demonstrated using a BY-2 plant cell suspension culture model. Environ. Exp. Bot. 2013, 91:1-11.
Rahman H., Jagadeeshselvam N., Valarmathi R., Sachin B., Sasikala R., Senthil N., Sudhakar D., Robin S., Muthurajan R. Transcriptome analysis of salinity responsiveness in contrasting genotypes of finger millet (Eleusine coracana L.) through RNA-sequencing. Plant Mol. Biol. 2014, 85:485-503.
Reginato M., Travaglia C., Reinoso H., Garello F., Luna V. Salt mixtures induce anatomical modifications in the halophyte Prosopis strombulifera (Fabaceae: Mimosoideae). Flora 2016, 218:75-85.
Sairam R.K., Roa K.V., Srivastava G.C. Differential response of wheat cultivar genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Sci. 2002, 163:1037-1048.
Sanevas N., Sunohara Y., Matsumoto H. Characterization of reactive oxygen species-involved oxidative damage in Hapalosiphon species crude extract-treated wheat and onion roots. Weed Biol. Manag. 2007, 7:172-177.
Sapeta H., Costa J.M., Lourenco T., Maroco J., van der Linde P. Drought stress response in Jatropha curcas: growth and physiology. Environ. Exp. Bot. 2013, 85:76-84.
Satish L., Ceasar S.A., Shilpha J., Rency A.S., Rathinapriya P., Ramesh M. Direct plant regeneration from in vitro-derived shoot apical meristems of finger millet (Eleusine coracana (L.) Gaertn.). In Vitro Cell Dev. Biol. Plant 2015, 51:192-200.
Satish L., Rathinapriya P., Rency S.A., Ceasar S.A., Pandian S., Rameshkumar R., Ramesh M. Somatic embryogenesis and regeneration using Graciliria edulis and Padina boergesenii seaweed liquid extracts and genetic fidelity in finger millet (Eleusine coracana). J. Appl. Phycol. 2015, 10.1007/s10811-015-0696-0.
Satish L., Rency A.S., Rathinapriya P., Ceasar S.A., Pandian S., Rameshkumar R., Rao T.B., Balachandran S.M., Ramesh M. Influence of plant growth regulators and spermidine on somatic embryogenesis and plant regeneration in four Indian genotypes of finger millet (Eleusine coracana (L.) Gaertn). Plant Cell Tissue Organ Culture 2016, 124:15-31.
Shi D., Sheng Y. Effect of various salt-alkaline mixed stress conditions on sunflower seedlings and analysis of their stress factors. Environ. Exp. Bot. 2005, 54:8-21.
Slama I., M'Rabet R., Ksouri R., Talbi O., Debez A., Abdelly C. Water deficit stress applied only or combined with salinity affects physiological parameters and antioxidant capacity in Sesuvium portulacastrum. Flora 2015, 213:69-76.
Striker G.G., Teakle N.L., Colmer T.D., Barrett-Lennard E.G. Growth responses of Melilotus siculus accessions to combined salinity and root-zone hypoxia are correlated with differences in tissue ion concentrations and not differences in root aeration. Environ. Exp. Bot. 2015, 109:89-98.
Tabot P.T., Adams J.B. Early responses of Bassia diffusa (Thunb.) Kuntze to submergence for different salinity treatments. S. Afr. J. Bot. 2013, 84:19-29.
Tlig T., Gorai M., Neffati M. Germination responses of Diplotaxis harra to temperature and salinity. Flora 2008, 203:421-428.
Zhong R., Ripperger A., Ye Z. Ectopic deposition of lignin in the pith of stems of two Arabidopsis mutants. Plant Physiol. 2000, 123:59-69.
Zorb C., Muhling K.H., Kutschera U., Geilfus C.M. Salinity stiffens the epidermal cell walls of salt-stressed maize leaves: is the epidermis growth-restricting?. PLoS One 2015, 10(3):e0118406. 10.1371/journal.pone.0118406.
Zuazo V.H.D., Raya A.M., Ruiz J.A. Impact of salinity on the fruit yield of mango (Mangifera indica L. cv. 'Osteen'). Eur. J. Agron. 2004, 21:323-334.
