Identification of putative QTLs for seedling stage phosphorus starvation response in finger millet (Eleusine coracana L. Gaertn.) by association mapping and cross species synteny analysis
Ramakrishnan, M.; Stanislaus, Antony Ceasar; Vinodet al.
[en] A germplasm assembly of 128 finger millet genotypes from 18 countries was evaluated for
seedling-stage phosphorus (P) responses by growing them in P sufficient (Psuf) and P deficient
(Pdef) treatments. Majority of the genotypes showed adaptive responses to low P condition.
Based on phenotype behaviour using the best linear unbiased predictors for each
trait, genotypes were classified into, P responsive, low P tolerant and P non-responsive
types. Based on the overall phenotype performance under Pdef, 10 genotypes were identified
as low P tolerants. The low P tolerant genotypes were characterised by increased shoot
and root length and increased root hair induction with longer root hairs under Pdef, than
under Psuf. Association mapping of P response traits using mixed linear models revealed
four quantitative trait loci (QTLs). Two QTLs (qLRDW.1 and qLRDW.2) for low P response
affecting root dry weight explained over 10% phenotypic variation. In silico synteny analysis
across grass genomes for these QTLs identified putative candidate genes such as Ser-Thr
kinase and transcription factors such as WRKY and basic helix-loop-helix (bHLH). The
QTLs for response under Psuf were mapped for traits such as shoot dry weight (qHSDW.1)
and root length (qHRL.1). Putative associations of these QTLs over the syntenous regions
on the grass genomes revealed proximity to cytochrome P450, phosphate transporter and
pectin methylesterase inhibitor (PMEI) genes. This is the first report of the extent of phenotypic
variability for P response in finger millet genotypes during seedling-stage, along with
the QTLs and putative candidate genes associated with P starvation tolerance
Disciplines :
Biotechnology
Author, co-author :
Ramakrishnan, M.
Stanislaus, Antony Ceasar ; Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Vinod
Duraipandiyan, V.
Ajeesh Krishna, TP
Upadhyaya
Ignacimuthu, S.
Language :
English
Title :
Identification of putative QTLs for seedling stage phosphorus starvation response in finger millet (Eleusine coracana L. Gaertn.) by association mapping and cross species synteny analysis
Publication date :
August 2017
Journal title :
PLoS ONE
eISSN :
1932-6203
Publisher :
Public Library of Science, United States - California
Volume :
12
Issue :
8
Pages :
1-27
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Grant number: BT/PR15011/AGR/02/772/2010. http://www.dbtindia.nic.in/ (Department of Biotechnology)
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