Insights into the molecular basis of biocontrol of Brassica pathogens by Bacillus amyloliquefaciens UCMB5113 lipopeptides

Asari, S.; Ongena, Marc; Debois, Delphine; De Pauw, Edwin; Chen, K.; Bejai, S.; Meijer, J.

doi:10.1093/aob/mcx089

Article (Scientific journals)

Insights into the molecular basis of biocontrol of Brassica pathogens by Bacillus amyloliquefaciens UCMB5113 lipopeptides

Asari, S.; Ongena, Marc; Debois, Delphine et al.

2017 • In Annals of Botany, 120 (4), p. 551-562

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/219791

DOI
10.1093/aob/mcx089

PubMed
28961818

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Keywords :

Antagonism; Arabidopsis thaliana; Bacillus amyloliquefaciens; Alternaria brassicicola; Arabidopsis; Brassica; Brassica napus; Rhizobium; Alternaria; Antifungal Agents; Disease Resistance; Host-Pathogen Interactions; Lipopeptides; Plant Leaves; Plant Roots

Abstract :

[en] Background and Aims Certain micro-organisms can improve plant protection against pathogens. The protective effect may be direct, e.g. due to antibiotic compounds, or indirect, by priming of plant defence as induced systemic resistance (ISR). The plant growth-promoting rhizobacterium Bacillus amyloliquefaciens UCMB5113 shows potential for disease management of oilseed rape. To investigate the mode of action of this protection, especially in relation to jasmonic acid-dependent ISR, Bacillus UCMB5113 was tested with Arabidopsis thaliana mutants and several important fungal pathogens of Brassica species. Methods Secreted lipopeptide fractions from Bacillus UCMB5113, together with synthetic peptide mimics, were evaluated for their effects on fungal phytopathogens and A. thaliana. The structures of secreted lipopeptides were analysed using mass spectrometry. Plant mutants and reporter lines were used to identify signalling steps involved in disease suppression by lipopeptides. Key Results In plate tests Bacillus UCMB5113 and lipopeptide extracts suppressed growth of several fungal pathogens infecting Brassica plants. Separation of secreted lipopeptides using reversed-phase high-performance liquid chromatography revealed several fractions that inhibited fungal growth. Analysis by mass spectrometry identified the most potent compounds as novel linear forms of antifungal fengycins, with synthetic peptide mimics confirming the biological activity. Application of the lipopeptide extracts on Arabidopsis roots provided systemic protection against Alternaria brassicicola on leaves. Arabidopsis signalling mutants and PDF1.2 and VSP2 promoter-driven GUS lines indicated that the lipopeptide fraction involved jasmonic-acid-dependent host responses for suppression of fungal growth indicative of ISR. Conclusions The ability of Bacillus UCMB5113 to counteract pathogens using both antagonistic lipopeptides and through ISR provides a promising tool for sustainable crop production. © The Author 2017.

Disciplines :

Microbiology

Author, co-author :

Asari, S.; Department of Plant Biology, Uppsala Biocenter, Linndan Center for Plant Biology, Swedish University of Agricultural Sciences, Uppsala, Sweden

Ongena, Marc ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies

Debois, Delphine ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)

De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)

Chen, K.; Department of Plant Biology, Uppsala Biocenter, Linndan Center for Plant Biology, Swedish University of Agricultural Sciences, Uppsala, Sweden, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China

Bejai, S.; Department of Plant Biology, Uppsala Biocenter, Linndan Center for Plant Biology, Swedish University of Agricultural Sciences, Uppsala, Sweden

Meijer, J.; Department of Plant Biology, Uppsala Biocenter, Linndan Center for Plant Biology, Swedish University of Agricultural Sciences, Uppsala, Sweden

Language :

English

Title :

Insights into the molecular basis of biocontrol of Brassica pathogens by Bacillus amyloliquefaciens UCMB5113 lipopeptides

Publication date :

2017

Journal title :

Annals of Botany

ISSN :

0305-7364

eISSN :

1095-8290

Publisher :

Oxford University Press

Volume :

120

Issue :

Pages :

551-562

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 05 February 2018

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