Article (Scientific journals)
Development of a non-toxic and non-denaturing formulation process for encapsulation of SDF-1α into PLGA/PEG-PLGA nanoparticles to achieve sustained release
Haji Mansor, Muhammad; Najberg, Mathie; Contini, Aurélien et al.
2018In European Journal of Pharmaceutics and Biopharmaceutics, 125, p. 38-50
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Keywords :
biomaterial; nanomedicine
Abstract :
[en] Chemokines are known to stimulate directed migration of cancer cells. Therefore, the strategy involving gradual chemokine release from polymeric vehicles for trapping cancer cells is of interest. In this work, the chemokine stromal cell-derived factor-1α (SDF-1α) was encapsulated into nanoparticles composed of poly-(lactic-co-glycolic acid) (PLGA) and a polyethylene glycol (PEG)-PLGA co-polymer to achieve sustained release. SDF-1α and lysozyme as a model protein, were firstly precipitated to promote their stability upon encapsulation. A novel phase separation method utilising a non-toxic solvent in the form of isosorbide dimethyl ether was developed for the individual encapsulation of SDF-1α and lysozyme precipitates. Uniform nanoparticles of 200–250 nm in size with spherical morphologies were successfully synthesised under mild formulation conditions and conveniently freeze-dried in the presence of hydroxypropyl-β-cyclodextrin as a stabiliser. The effect of PLGA carboxylic acid terminal capping on protein encapsulation efficiency and release rate was also explored. Following optimisation, sustained release of SDF-1α was achieved over a period of 72 h. Importantly, the novel encapsulation process was found to induce negligible protein denaturation. The obtained SDF-1α nanocarriers may be subsequently incorporated within a hydrogel or other scaffolds to establish a chemokine concentration gradient for the trapping of glioblastoma cells. © 2017 Elsevier B.V.
Research center :
Center for Education and Research on Macromolecules (CERM)
CESAM - Complex and Entangled Systems from Atoms to Materials - ULiège
Disciplines :
Chemistry
Materials science & engineering
Pharmacy, pharmacology & toxicology
Author, co-author :
Haji Mansor, Muhammad;  University of Angers, University of Nantes, CRCINA INSERM, France > University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM)
Najberg, Mathie;  University of Angers, University of Nantes, CRCINA INSERM, France > Universidade de Santiago de Compostela, Departamento de Farmacologia, Farmacia y Tecnología Farmacéutica, R&D Pharma Group, Spain
Contini, Aurélien;  University of Angers, University of Nantes, CRCINA INSERM, France
Alvarez-Lorenzo, Carmen;  Universidade de Santiago de Compostela, Departamento de Farmacologia, Farmacia y Tecnología Farmacéutica, R&D Pharma Group, Spain
Garcion, Emmanuel;  University of Angers, University of Nantes, CRCINA INSERM, France
Jérôme, Christine  ;  University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM)
Boury, Frank;  University of Angers, CRCINA INSERM-UMR1232 Cancer and Immunology Research Centre, France
Language :
English
Title :
Development of a non-toxic and non-denaturing formulation process for encapsulation of SDF-1α into PLGA/PEG-PLGA nanoparticles to achieve sustained release
Publication date :
April 2018
Journal title :
European Journal of Pharmaceutics and Biopharmaceutics
ISSN :
0939-6411
eISSN :
1873-3441
Publisher :
Elsevier B.V.
Volume :
125
Pages :
38-50
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
FEDER - Fonds Européen de Développement Régional [BE]
The Erasmus Mundus Joint Doctorate "Nanofar"
French Region "Pays de la Loire"
Spanish Ministry of Economy and Competitiveness (Mineco)
French Ministry of Higher Education and Research
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since 22 January 2018

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