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Atypical structure and subunit composition of respiratory complexes in Euglena gracilis
Miranda Astudillo, Héctor Vicente; Yadav, Sathish; Colina-Tenorio, Lilia et al.
2016Photosynthetic and Respiratory complexes, from structure to function
 

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Abstract :
[en] Euglena gracilis, a non-parasitic secondary green alga related to trypanosomes, has a complex mitochondrial oxidative phosphorylation system constituted by atypical respiratory enzymes (complexes I - V). Recently, the analysis of the subunit composition of respiratory complexes by 2D BN/SDS PAGE has shown that at least 41 of the non-canonical subunits reported in trypanosomes are also present in this alga along with 48 classical subunits described in other eukaryotes including green plants. In the present study the complexes I, III, IV and V were further purified from isolated mitochondria using liquid chromatography after solubilization with n-dodecyl-maltoside. Using a 3D BN/SDS/SDS PAGE analysis, we resolved their subunit composition and confirmed the atypical subunit composition of Euglena respiratory complexes. The apparent molecular mass of purified complexes I and V (1.5 and 2.2MDa, respectively) is far above the classical ones. Single-particle analysis from transmission electron microscopy revealed some unusual features for complexes I, IV and V, including smaller angles between monomers and additional membrane extensions for the ATP synthase. Complex I also shows an unusual long matricial arm. Meanwhile the complex IV shows an atypical shape compared with the bovine one.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Miranda Astudillo, Héctor Vicente ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues
Yadav, Sathish
Colina-Tenorio, Lilia
Bouillenne, Fabrice
Degand, Hervé
Morsomme, Pierre
Boekema, Egbert
González-Halphen, Diego
Cardol, Pierre  
Language :
English
Title :
Atypical structure and subunit composition of respiratory complexes in Euglena gracilis
Publication date :
14 August 2016
Event name :
Photosynthetic and Respiratory complexes, from structure to function
Event place :
Verviers, Belgium
Event date :
from 13th to 16th August 2016
Audience :
International
Available on ORBi :
since 22 January 2018

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