Human bone marrow harbors cells with neural crest-associated characteristics like human adipose and dermis tissues.

Adipose Tissue/cytology/metabolism; Adult; Animals; Biomarkers/metabolism; Bone Marrow Cells/cytology/metabolism; Cell Differentiation; Chick Embryo; Dermis/cytology/metabolism; Female; Gene Expression; Humans; Melanocytes/cytology/metabolism; Mesenchymal Stem Cell Transplantation; Mesenchymal Stromal Cells/cytology/metabolism; Microinjections; Nerve Tissue Proteins/genetics/metabolism; Nestin/genetics/metabolism; Neural Crest/cytology/growth & development/metabolism; Neural Stem Cells/cytology/metabolism; Neurons/cytology/metabolism; Nuclear Proteins/genetics/metabolism; RNA-Binding Proteins/genetics/metabolism; Receptors, Nerve Growth Factor/genetics/metabolism; SOX9 Transcription Factor/genetics/metabolism; Schwann Cells/cytology/metabolism; Snail Family Transcription Factors/genetics/metabolism; Transcription Factor Brn-3A/genetics/metabolism; Twist-Related Protein 1/genetics/metabolism

Abstract :

[en] Adult neural crest stem-derived cells (NCSC) are of extraordinary high plasticity and promising candidates for use in regenerative medicine. Several locations such as skin, adipose tissue, dental pulp or bone marrow have been described in rodent, as sources of NCSC. However, very little information is available concerning their correspondence in human tissues, and more precisely for human bone marrow. The main objective of this study was therefore to characterize NCSC from adult human bone marrow. In this purpose, we compared human bone marrow stromal cells to human adipose tissue and dermis, already described for containing NCSC. We performed comparative analyses in terms of gene and protein expression as well as functional characterizations. It appeared that human bone marrow, similarly to adipose tissue and dermis, contains NESTIN+ / SOX9+ / TWIST+ / SLUG+ / P75NTR+ / BRN3A+/ MSI1+/ SNAIL1+ cells and were able to differentiate into melanocytes, Schwann cells and neurons. Moreover, when injected into chicken embryos, all those cells were able to migrate and follow endogenous neural crest migration pathways. Altogether, the phenotypic characterization and migration abilities strongly suggest the presence of neural crest-derived cells in human adult bone marrow.

Disciplines :

Hematology

Author, co-author :

Coste, Cécile

Neirinckx, Virginie ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques

Sharma, Anil

Agirman, Gulistan ; Université de Liège - ULiège > GIGA - Neurosciences

Rogister, Bernard ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine

FOGUENNE, Jacques ; Centre Hospitalier Universitaire de Liège - CHU > Service d'hématologie biologique et immuno-hématologie

Lallemend, François

Gothot, André ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie de la coagulation et de l'hémostase

Wislet, Sabine ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine

Language :

English

Title :

Human bone marrow harbors cells with neural crest-associated characteristics like human adipose and dermis tissues.

Publication date :

2017

Journal title :

PLoS ONE

eISSN :

1932-6203

Publisher :

Public Library of Science, United States - California

Volume :

Issue :

Pages :

e0177962

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 22 January 2018

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