Article (Scientific journals)
Physiological characterization of a plant mitochondrial Calcium uniporter in Vitro and in Vivo
Teardo, E.; Carraretto, L.; Wagner, S. et al.
2017In Plant Physiology, 173 (2), p. 1355-1370
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Keywords :
Arabidopsis protein; MCU1 protein, Arabidopsis; MCU2 protein, Arabidopsis; MICU protein, Arabidopsis; Arabidopsis; Arabidopsis Proteins; Calcium; Calcium Channels; Calcium-Binding Proteins; Gene Expression Regulation, Plant; Mitochondria; Mutation; Phylogeny; Plant Proteins; Plant Roots
Abstract :
[en] Over the recent years, several proteins that make up the mitochondrial calcium uniporter complex (MCUC) mediating Ca2+ uptake into the mitochondrial matrix have been identified in mammals, including the channel-forming protein MCU. Although six MCU gene homologs are conserved in the model plant Arabidopsis (Arabidopsis thaliana) in which mitochondria can accumulate Ca2+, a functional characterization of plant MCU homologs has been lacking. Using electrophysiology, we show that one isoform, AtMCU1, gives rise to a Ca2+-permeable channel activity that can be observed even in the absence of accessory proteins implicated in the formation of the active mammalian channel. Furthermore, we provide direct evidence that AtMCU1 activity is sensitive to the mitochondrial calcium uniporter inhibitors Ruthenium Red and Gd3+, as well as to the Arabidopsis protein MICU, a regulatory MCUC component. AtMCU1 is prevalently expressed in roots, localizes to mitochondria, and its absence causes mild changes in Ca2+ dynamics as assessed by in vivo measurements in Arabidopsis root tips. Plants either lacking or overexpressing AtMCU1 display root mitochondria with altered ultrastructure and show shorter primary roots under restrictive growth conditions. In summary, our work adds evolutionary depth to the investigation of mitochondrial Ca2+ transport, indicates that AtMCU1, together with MICU as a regulator, represents a functional configuration of the plant mitochondrial Ca2+ uptake complex with differences to the mammalian MCUC, and identifies a new player of the intracellular Ca2+ regulation network in plants. © 2017 American Society of Plant Biologists. All rights reserved.
Disciplines :
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Teardo, E.;  Department of Biology, University of Padova, Padova, Italy, CNR Institute of Neuroscience, Padova, Italy, Department of Biomedical Sciences, University of Padua, Padova, Italy
Carraretto, L.;  Department of Biology, University of Padova, Padova, Italy
Wagner, S.;  Plant Energy Biology Lab, Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Bonn, Germany
Formentin, E.;  Department of Biology, University of Padova, Padova, Italy
Behera, S.;  Department of Biosciences, University of Milan, Milan, Italy
De Bortoli, S.;  Department of Biology, University of Padova, Padova, Italy
Larosa, Véronique ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues
Fuchs, P.;  Plant Energy Biology Lab, Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Bonn, Germany
Lo Schiavo, F.;  Department of Biology, University of Padova, Padova, Italy
Raffaello, A.;  Department of Biomedical Sciences, University of Padova, Padova, Italy
Rizzuto, R.;  Department of Biomedical Sciences, University of Padova, Padova, Italy
Costa, A.;  Department of Biosciences, University of Milan, Milan, Italy, Institute of Biophysics, Consiglio Nazionale delle Ricerche, Milan, Italy
Schwarzländer, M.;  Plant Energy Biology Lab, Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Bonn, Germany
Szabò, I.;  Department of Biology, University of Padova, Padova, Italy, CNR Institute of Neuroscience, Padova, Italy, Department of Biomedical Sciences, University of Padua, Padova, Italy
More authors (4 more) Less
Language :
English
Title :
Physiological characterization of a plant mitochondrial Calcium uniporter in Vitro and in Vivo
Publication date :
2017
Journal title :
Plant Physiology
ISSN :
0032-0889
eISSN :
1532-2548
Publisher :
American Society of Plant Biologists
Volume :
173
Issue :
2
Pages :
1355-1370
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 16 January 2018

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