Letter to the editor (Scientific journals)
Merged heme and non-heme manganese cofactors for a dual antioxidant surveillance in photosynthetic organisms
Squarcina, A.; Sorarù, A.; Rigodanza, F. et al.
2017In ACS Catalysis, 7 (3), p. 1971-1976
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Keywords :
Antioxidant catalysis; Artificial enzymes; Catalase; Manganese catalysis; Photoprotection; Superoxide dismutase; Algae; Antioxidants; Catalysis; Detoxification; Enzymes; Manganese; Oxygen; Catalytic performance; Chlamydomonas reinhardtii; Photosynthetic organisms; Physiological condition; Super oxide dismutase; Porphyrins
Abstract :
[en] The coupling of a polycationic Mn(III)-porphyrin, with a dinuclear Mn2(II,II)L2 core (HL = 2-{[[di(2-pyridyl)methyl](methyl)amino]-methyl}phenol), results in a dual Superoxide Dismutase (SOD) and Catalase (CAT) functional analogue, Mn2L2Pn+, enabling a detoxification cascade of the superoxide anion and hydrogen peroxide into benign H2O and O2. The SOD/CAT artificial manifolds, joined in one asset, exhibit a peak catalytic performance under physiological conditions, with log kcat(O2 •-) ≥ 7 and kcat(H2O2)/KM = 1890. The dual-enzyme (dizyme) concept allows for a builtin self-protection against the irreversible bleaching of the porphyrin unit (>75% protection), readily induced by H2O2 (200 μM, 20 equiv, in buffer solution, pH 7.8). We show herein that incubation of the photosynthetic green algae, Chlamydomonas reinhardtii, with the synthetic dizyme (as low as 0.1 μM), prevents H2O2 accumulation under high-light illumination conditions, thus providing antioxidant surveillance and photoprotection. (Chemical Equation Presented). © 2017 American Chemical Society.
Disciplines :
Phytobiology (plant sciences, forestry, mycology...)
Chemistry
Author, co-author :
Squarcina, A.;  ITM-CNR, Department of Chemical Sciences, University of Padova, Via Marzolo 1, Padova, Italy
Sorarù, A.;  ITM-CNR, Department of Chemical Sciences, University of Padova, Via Marzolo 1, Padova, Italy
Rigodanza, F.;  Department of Chemical and Pharmaceutical Sciences, University of Trieste, Via Giorgieri 1, Trieste, Italy
Carraro, M.;  ITM-CNR, Department of Chemical Sciences, University of Padova, Via Marzolo 1, Padova, Italy
Brancatelli, G.;  Department of Chemical and Pharmaceutical Sciences, University of Trieste, Via Giorgieri 1, Trieste, Italy
Carofiglio, T.;  ITM-CNR, Department of Chemical Sciences, University of Padova, Via Marzolo 1, Padova, Italy
Geremia, S.;  Department of Chemical and Pharmaceutical Sciences, University of Trieste, Via Giorgieri 1, Trieste, Italy
Larosa, Véronique ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues
Morosinotto, T.;  Department of Biology, University of Padova, via Bassi 58b, Padova, Italy
Bonchio, M.;  ITM-CNR, Department of Chemical Sciences, University of Padova, Via Marzolo 1, Padova, Italy
Language :
English
Title :
Merged heme and non-heme manganese cofactors for a dual antioxidant surveillance in photosynthetic organisms
Publication date :
2017
Journal title :
ACS Catalysis
eISSN :
2155-5435
Publisher :
American Chemical Society
Volume :
7
Issue :
3
Pages :
1971-1976
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Fondazione Cariparo [IT]
UNIPD - University of Padua [IT]
COST - European Cooperation in Science and Technology [BE]
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since 16 January 2018

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