Reference : Merged heme and non-heme manganese cofactors for a dual antioxidant surveillance in p...
Scientific journals : Letter to the editor
Physical, chemical, mathematical & earth Sciences : Chemistry
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
http://hdl.handle.net/2268/218427
Merged heme and non-heme manganese cofactors for a dual antioxidant surveillance in photosynthetic organisms
English
Squarcina, A. [ITM-CNR, Department of Chemical Sciences, University of Padova, Via Marzolo 1, Padova, Italy]
Sorarù, A. [ITM-CNR, Department of Chemical Sciences, University of Padova, Via Marzolo 1, Padova, Italy]
Rigodanza, F. [Department of Chemical and Pharmaceutical Sciences, University of Trieste, Via Giorgieri 1, Trieste, Italy]
Carraro, M. [ITM-CNR, Department of Chemical Sciences, University of Padova, Via Marzolo 1, Padova, Italy]
Brancatelli, G. [Department of Chemical and Pharmaceutical Sciences, University of Trieste, Via Giorgieri 1, Trieste, Italy]
Carofiglio, T. [ITM-CNR, Department of Chemical Sciences, University of Padova, Via Marzolo 1, Padova, Italy]
Geremia, S. [Department of Chemical and Pharmaceutical Sciences, University of Trieste, Via Giorgieri 1, Trieste, Italy]
Larosa, Véronique mailto [Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues >]
Morosinotto, T. [Department of Biology, University of Padova, via Bassi 58b, Padova, Italy]
Bonchio, M. [ITM-CNR, Department of Chemical Sciences, University of Padova, Via Marzolo 1, Padova, Italy]
2017
ACS Catalysis
American Chemical Society
7
3
1971-1976
Yes (verified by ORBi)
International
2155-5435
[en] Antioxidant catalysis ; Artificial enzymes ; Catalase ; Manganese catalysis ; Photoprotection ; Superoxide dismutase ; Algae ; Antioxidants ; Catalysis ; Detoxification ; Enzymes ; Manganese ; Oxygen ; Catalytic performance ; Chlamydomonas reinhardtii ; Photosynthetic organisms ; Physiological condition ; Super oxide dismutase ; Porphyrins
[en] The coupling of a polycationic Mn(III)-porphyrin, with a dinuclear Mn2(II,II)L2 core (HL = 2-{[[di(2-pyridyl)methyl](methyl)amino]-methyl}phenol), results in a dual Superoxide Dismutase (SOD) and Catalase (CAT) functional analogue, Mn2L2Pn+, enabling a detoxification cascade of the superoxide anion and hydrogen peroxide into benign H2O and O2. The SOD/CAT artificial manifolds, joined in one asset, exhibit a peak catalytic performance under physiological conditions, with log kcat(O2 •-) ≥ 7 and kcat(H2O2)/KM = 1890. The dual-enzyme (dizyme) concept allows for a builtin self-protection against the irreversible bleaching of the porphyrin unit (>75% protection), readily induced by H2O2 (200 μM, 20 equiv, in buffer solution, pH 7.8). We show herein that incubation of the photosynthetic green algae, Chlamydomonas reinhardtii, with the synthetic dizyme (as low as 0.1 μM), prevents H2O2 accumulation under high-light illumination conditions, thus providing antioxidant surveillance and photoprotection. (Chemical Equation Presented). © 2017 American Chemical Society.
CPDA141843/14, Università degli Studi di Padova; CM1205; Fondazione Cassa di Risparmio di Padova e Rovigo
http://hdl.handle.net/2268/218427
10.1021/acscatal.7b00004

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