[en] Pigs were fed protein-free diets containing different pea inner fiber (PIF) isolates (from wrinkled or smooth peas, unprocessed or micronized) or different PIF levels (from 50 to 200 g/kg diet), and the flows of endogenous N and amino acids were measured at the ileum level. The flows were better correlated to the water-holding capacity (WHC) of the diet than to fiber intake (R2 = 0.996 vs 0.76, respectively). The relationship with WHC was of the exponential type, with a high increase when the WHC exceeded 3 g of water/g diet. A similar pattern and correlation (R2 = 0.98) was obtained for the ileal flow of nucleobases (markers of epithelial cells and bacteria), whereas the relationships with crude mucus (R2 = 0.96) and diaminopimelic acid (marker of bacteria, R2 = 0.55) were linear. N retention by pigs fed with 0, 30, 60, or 90 g/kg of dry matter (DM) of the same protein isolates did not differ significantly in pigs receiving a 40 or 160 g of PIF/kg diet, despite significant differences in ileal N and amino acid flows between the two fiber levels (2.0 and 3.1 g of N/kg of DM intake, on average). These results and those of the flow of endogenous N compounds suggest an effect of the swollen fibers on the intestinal wall rather than a disturbance of the digestive processes.
AOAC. Official Methods of Analysis, 13th ed.; AOAC: Washington, DC, 1980.
Canibe, N.; Bach Knudsen, K. E. Digestibility of dried and toasted peas in pigs. 1. Ileal and total tract digestibilities of carbohydrates. Anim. Feed Sci. Technol. 1997, 64, 293-310.
Cassidy, M.; Lightfoot, F.; Grau, L.; Story, J.; Kritchevsky, D.; Vahouny, G. Effect of chronic intake of dietary fibres on the ultrastructural topography of rat jejunum and colon: A scanning electron microscropy study. Am. J. Clin. Nutr. 1981, 34, 218-228.
Decuypere, J.; Spriet, S.; van Gils, L. Influence of the water-holding capacity of the feed on the precaecal and faecal apparent digestibility in pigs. In Proceedings of 6th International Symposium on Digestive Physiology in Pigs; Souffrant, W., Hagemeister, H., Eds.; EAAP Publ. 80, Rome, Italy; Pudoc: Wageningen, The Netherlands, 1994; pp 125-128.
Dierick, N.; Vervaeke, I.; Decuypere, J.; Henderickx, H. Influence de la nature et du niveau des fibres brutes sur la digestibilité iléale et fécale apparente de la matière sèche, des protéines et des acides aminés et sur la rétentions azotée chez les porcs (Effect of the nature and level of crude fibre on apparent ileal and faecal digestibility of dry matter, protein and amino acids and on nitrogen retention in pigs). Rev. Agric. 1983, 36, 1691-1711.
Englyst, H.; Quigley, M.; Hudson, G.; Cummings, J. Determination of dietary fibre as nonstarch polysaccharides by gasliquid chromatography. Analyst 1992, 117, 1707-1714.
Everts, H.; Dekker, R.; Smiths, B.; Cone, J. The digestion of corn and native pea starch in the small intestine of pigs. Proc. Nutr. Soc. 1996, 55, 59A.
François, E.; Thill, N.; Théwis, A. Méthode rapide de dosage de l'oxyde de chrome dans les aliments, les fèces et les contenus digestifs par titrage après oxidation nitro-perchlorique (A rapid volumetric method for the determination of chromium sesquioxide in feeds, faeces and digesta after nitroperchloric digestion). Ann. Zootech. 1978, 27, 355-361.
Gee, J.; Lee-Finglas, W.; Wortley, G.; Johnson, I. Fermentable carbohydrates elevate plasma enteroglucagon but high viscosity is also necessary to stimulate small bowel mucosal cell proliferation in rats. J. Nutr. 1996, 126, 373-379.
Grala, W.; Verstegen, M.; van Leeuwen, P.; Huisman, J.; Jansman, A.; Tamminga, S. Nitrogen balance of pigs as affected by feedstuffs causing different endogenous nitrogen flow at the terminal ileum. Livest. Prod. Sci. 1997, 48, 143-156.
Hara, H.; Suzuki, K.; Kobayashi, S.; Kasai, T. Fermentable property of dietary fibre may not determine cecal and colonic mucosal growth in fibre-fed rats. J. Nutr. Biochem. 1996, 7, 549-555.
Jacobs, L. Effects of dietary fiber on mucosal growth and cell proliferation in the small intestine of the rat: A comparison of oat bran, pectin and guar with total fiber deprivation. Am. J. Clin. Nutr. 1983, 37, 954-960.
Jin, L.; Reynolds, L.; Redmer, D.; Caton, J.; Crenshaw, J. Effects of dietary fiber on intestinal growth, cell proliferation and morphology in growing pigs. J. Anim. Sci. 1994, 72, 2270-2278.
Johansen, H.; Bach Knudsen, K.; Sandström, B.; Skjøth, F. Effects of varying content of soluble dietary fibre from wheat flour and oat milling fractions on gastric emptying in pigs. Br. J. Nutr. 1996, 75, 339-351.
Kyriazakis, I.; Emmans, G. The volontary feed intake of pigs given feeds based on wheat bran, dried citrus pulp and grass meal, in relation to measurements of feed bulk. Br. J. Nutr. 1995, 73, 191-208.
Larsen, F.; Moughan, P.; Wilson, M. Dietary fiber viscosity and endogenous protein excretion at the terminal ileum of growing rats. J. Nutr. 1993, 123, 1898-1904.
Larsen, F.; Wilson, M.; Moughan, P. Dietary fiber viscosity and amino acid digestibility, proteolytic digestive enzyme activity and digestive organ weights in growing rats. J. Nutr. 1994, 124, 833-841.
Lassalas, B.; Jouany, J.; Broudiscou, L. Dosage des bases puriques et pyrimidiques par Chromatographie liquide à haute performance (Determination of the puric and pyrimidic bases by high-performance liquid chromatography). Ann. Zootech. 1993, 42, 170-171.
Leterme, P.; van Leeuwen, P.; Théwis, A.; Huisman, J. Chemical composition of pea inner fibre isolates and their effect on the endogenous digestive secretions in pigs. J. Sci. Food Agric. 1996a, 72, 127-134.
Leterme, P.; Théwis, A.; François, E.; van Leeuwen, P.; Wathelet, B.; Huisman, J. The use of 15N-labeled dietary proteins for determining true ileal amino acid digestibilities is limited by their rapid recycling in the endogenous secretions of pigs. J. Nutr. 1996b, 126, 2188-2199.
Leterme, P.; Monmart, T.; Morandi, P.; Théwis, A. Oral and parenteral N nutrition vs N-free nutrition on the ileal endogenous losses and on the molecular weight distribution of the nitrogenous compounds in the ileal digesta of pigs. J. Sci. Food Agric. 1996c, 71, 265-271.
Leterme, P.; Rossi, F.; Théwis, A. Effet des protéines et des fibres alimentaires sur les pertes iléales d'acides aminés endogènes chez le porc (Effect of dietary proteins and fibres on the ileal endogenous amino acid losses in pigs). Journ. Rech. Porcine Fr. 1997, 29, 243-248.
MacKenzie, S.; Tenaschuk, D. Quantitative formation of N (O, S)-heptafluorobutyryl isobutyl amino acids for gas chromatographic analyses. I. Esterification. J. Chromatogr. 1979a, 171, 195-208.
MacKenzie, S.; Tenaschuk, D. Quantitative formation of N (O, S)-heptafluorobutyryl isobutyl amino acids for gas chromatographic analyses. II. Acylation. J. Chromatogr. 1979b, 173, 53-63.
Mariscal-Landín, G.; Sève, B.; Colléaux, Y.; Lebreton, Y. Endogenous amino nitrogen, collected from pigs with end-to-end ileorectal anastomosis, is affected by the method of estimation and altered by dietary fiber. J. Nutr. 1995, 125, 136-146.
Matthews, D. Protein absorption: Development and present state of the subject; Wiley-Liss: New York, 1991.
Moore, R.; Kornegay, E.; Grayson, R.; Lindermann, M. Growth, nutrient utilization and intestinal morphology of pigs fed high-fiber diets. J. Anim. Sci. 1988, 66, 1570-1579.
Prosky, L.; Asp, N.; Schweizer, T.; De Vries, J.; Furda, I. Determination of insoluble, soluble and total dietary fiber in foods and food products. J. AOAC Int. 1988, 71, 1017-1023.
Ratcliffe, B. The role of the microflora in digestion. In In vitro digestion for pigs and poultry; Fuller, M. F., Ed., CAB: Wallingford, U.K., 1991; pp 19-34.
Sakata, T. Stimulatory effect of short-chain fatty acids on epithelial cell proliferation in the rat intestine: Possible explanation for the trophic effects of fermentable fibre, gut microbes and luminal trophic factors. Br. J. Nutr. 1987, 58, 95-103.
Sakata, T.; Miyakozawa, M. Effects of mucosal abrasion on epithelial cell loss and epithelial cell proliferation of pig distal colon in vitro. In Digestive physiology in pigs; Laplace, J. P., Fevrier, C., Barbeau, C., Eds.; EAAP Publ. 88, Rome, Italy; Pudoc: Wageningen, The Netherlands, 1997; pp 558-561.
SAS/STAT. User's Guide, version 6, 4th ed.; SAS Institute: Cary, NC, 1994; Vol. 2.
Schmidt-Willig, U.; Enss, M.; Coenen, M.; Gärtner, K.; Hedrich, H. Response of rat colonic mucosa to a high fiber diet. Ann. Nutr. Metab. 1996, 40, 343-350.
Sève, B.; Henry, Y. Protein utilization in nonruminants. In Proceedings of the 7th Symposium International on Protein Metabolism and Nutrition; Nunes, A., Portugal, A., Costa, J., Ribeiro, J., Eds.; EAAP Publ. 81, Rome, Italy; Pudoc: Wageningen, The Netherlands, 1996; pp 59-82.
Souffrant, W.; Février, C.; Laplace, J.; Hennig, U. Comparison of methods to estimate ileal endogenous nitrogen and amino acids in piglets. In Digestive physiology in pigs; Laplace, J., Février, C., Barbeau, A., Eds; EAAP Publ. 88, Rome, Italy: Pudoc: Wageningen, The Netherlands, 1997; pp 591-595.
Tetens, I.; Livesey, G.; Eggum, B. Effects of the type and level of dietary fibre supplements on nitrogen retention and excretion patterns. Br. J. Nutr. 1996, 75, 461-469.
van Leeuwen, P.; van Kleef, D.; van Kempen, G.; Huisman, J.; Verstegen, M. The post-valve T-caecum cannulation technique: an alternative method for chyme collection in pigs. J. Anim. Physiol. Anim. Nutr. 1991, 65, 183-193.
Wong, S.; O'Dea, K. Importance of physical form rather than viscosity in determinng the rate of starch hydrolysis in legumes. Am. J. Clin. Nutr. 1983, 37, 66-70.
Wünsche, J.; Völker, T.; Souffrant, W.; Borgmann, E. Bestimmung der Bakterien-N-Anteile im Kot und unterschiedlich gewonnenem Ileumchymus von Schweinen (Assessment of the bacteria N proportions in faeces and differently obtained ileal digesta in pigs). Arch. Anim. Nutr. 1991,41, 703-714.
Würsch, P.; del Vedovo, S.; Koellreutter, B. Cell structure and starch nature as key determinants of the digestion rate of starch in legume. Am. J. Clin. Nutr. 1986, 43, 25-29.
