Poster (Scientific congresses and symposiums)
Atypical composition and structure of the mitochondrial dimeric ATP synthase from Euglena gracilis
Miranda Astudillo, Héctor Vicente; Yadav, Sathish; Colina-Tenorio, Lilia et al.
2017Le 19 ème congrès du Groupe Français de Bioenergetique 2017
 

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Keywords :
Euglenozoa; Trypanosomatidae; Dimericmitochondrial complex V; F1FO ATP synthase; Electronmicroscopy; ATP synthasome
Abstract :
[en] Mitochondrial respiratory-chain complexes fromEuglenozoa comprise classical subunits described in other eukaryotes (i.e. mammals and fungi) and subunits that are restricted to Euglenozoa (e.g. Euglena gracilis and Trypanosoma brucei). Herewe studied the mitochondrial F1FO-ATP synthase (or Complex V) fromthe photosynthetic eukaryote E. gracilis in detail. The enzymewas purified by a two-step chromatographic procedure and its subunit compositionwas resolved by a three-dimensional gel electrophoresis (BN/SDS/SDS).Twenty-two different subunits were identified by mass-spectrometry analyses amongwhich the canonical α, β, γ, δ, ε, and OSCP subunits, and at least seven subunits previously found in Trypanosoma. The ADP/ATP carrierwas also associated to the ATP synthase into a dimericATP synthasome. Single-particle analysis by transmission electron microscopy of the dimeric ATP synthase indicated that the structures of both the catalytic and central rotor parts are conserved while other structural features are original. These new features include a large membrane-spanning re- gion joining the monomers, an external peripheral stalk and a structure that goes through the membrane and reaches the inter membrane space below the c-ring, the latter having not been reported for any mitochondrial F-ATPase.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Miranda Astudillo, Héctor Vicente ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues
Yadav, Sathish
Colina-Tenorio, Lilia
Bouillenne, Fabrice
Degand, Hervé
Morsomme, Pierre
González-Halphen, Diego
Boekema, Egbert J.
Cardol, Pierre  
Language :
English
Title :
Atypical composition and structure of the mitochondrial dimeric ATP synthase from Euglena gracilis
Publication date :
21 September 2017
Number of pages :
Poster
Event name :
Le 19 ème congrès du Groupe Français de Bioenergetique 2017
Event organizer :
Groupe Français de Bioenergetique
Event place :
Lacanau, France
Event date :
20-24 September 2017
Audience :
International
Available on ORBi :
since 18 December 2017

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