Article (Scientific journals)
Cox2A/Cox2B subunit interaction in Polytomella sp. cytochrome c oxidase: role of the Cox2B subunit extension
Jiménez-Suárez, Alejandra; Vázquez-Acevedo, Miriam; Miranda Astudillo, Héctor Vicente et al.
2017In Journal of Bioenergetics and Biomembranes, 49 (6), p. 453-461
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Keywords :
Polytomella sp; Chlamydomonas reinhardtii; Mitochondrial targeting signal; Cox2; Cytochromec oxidase biogenesis
Abstract :
[en] Subunit II of cytochrome c oxidase (Cox2) is usually encoded in the mitochondrial genome, synthesized in the organelle, inserted co-translationally into the inner mitochondrial membrane, and assembled into the respiratory complex. In chlorophycean algae however, the cox2 gene was split into the cox2a and cox2b genes, and in some algal species like Chlamydomonas reinhardtii and Polytomella sp. both fragmented genes migrated to the nucleus. The corresponding Cox2A and Cox2B subunits are imported into mitochondria forming a heterodimeric Cox2 subunit. When comparing the sequences of chlorophycean Cox2Aand Cox2B proteins with orthodox Cox2 subunits, a C-terminal extension in Cox2A and an N-terminal extension in Cox2B were identified. It was proposed that these extensions favor the Cox2A/Cox2B interaction. In vitro studies carried out in this work suggest that the removal of the Cox2B extension only partially affects binding of Cox2B to Cox2A.We conclude that this extension is dispensable, but when present it weakly reinforces the Cox2A/Cox2B interaction.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Jiménez-Suárez, Alejandra;  Universidad Nacional Autónoma de México > Instituto de Fisiología Celular > Departamento de GenéticaMolecular
Vázquez-Acevedo, Miriam;  Universidad Nacional Autónoma de México > Instituto de Fisiología Celular > Departamento de GenéticaMolecular
Miranda Astudillo, Héctor Vicente ;  Universidad Nacional Autónoma de México > Instituto de Fisiología Celular > Departamento de GenéticaMolecular
González-Halphen, Diego;  Universidad Nacional Autónoma de México > Instituto de Fisiología Celular > Departamento de GenéticaMolecular
Language :
English
Title :
Cox2A/Cox2B subunit interaction in Polytomella sp. cytochrome c oxidase: role of the Cox2B subunit extension
Publication date :
22 September 2017
Journal title :
Journal of Bioenergetics and Biomembranes
ISSN :
0145-479X
eISSN :
1573-6881
Publisher :
Kluwer Academic/Plenum Publishers, New York, United States - New York
Volume :
49
Issue :
6
Pages :
453-461
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 18 December 2017

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