Etude de la transmission du gammaherpèsvirus murin 4: importance de la glycoprotéine gp150 et développement de stratégies antivirales basées sur l'utilisation du cidofovir
[en] Gammaherpèsviruses are important pathogens in human medicine. Indeed, Epstein Barr virus and Kaposi Sarcoma associated herpesvirus are responsible for numerous lymphoproliferative diseases and cancers. Murid Herpesvirus-4 (MuHV-4) is usually used as a model for human gammaherpèsviruses. Until now, the major limitation of this model was the absence of transmission in laboratory animals. Recently, a sexual transmission of MuHV-4 has been described in laboratory mice. This observation allows, on the one hand, to study mechanisms underlying natural transmission, such as cellular trospism, importance of viral proteins, or immunity response during this part of the cycle. On the other hand, this transmission model allows to test efficiency of measures to reduce transmission. Indeed, herpesvirus are archetype of persistant viruses as infection persists lifelong once established. Studying mechanisms underlying transmission and how to use these mechanisms to reduce it efficiently is therefore of primordial importance.
In a first study, we focussed on the biological relevance of mechanisms described previously in vitro. Indeed, a MuHV-4 glycoprotein, gp150, has been shown to participate to the release of infectious particles from cells and to evasion of humoral immunity, two functions that could be important for the virus during transmission. Our results showed that gp150 is essential for an efficient sexual transmission of the MuHV-4. Indeed, gp150 promotes the release of infectious particles from infected vaginal epithelial cells. These results has been published in Journal of Virology in July 2017.
In a second study, the same model was used to test efficiency of an antiviral molecule to reduce sexual transmission of the MuHV-4. A nucleotide analogue, the cidofovir, was administrated to mice according to different protocols. A daily administration of cidofovir reduced drastically genital shedding, but also prevent completely infection of naive mice after sexual contact. Finally, a single injection of cidofovir, administered 24 hours after sexual contact, reduced significantly transmission.
Altogether, in this work, we used a transmission model of a gammaherpèsvirus in two differents studies: the first foccused on glycoprotein implicated during the transmission and established the importance of a single viral glycoprotein for an efficient transmission. The second study highlighted the efficiency of antiviral drugs to prevent infection by gammaherpesviruses.
Research Center/Unit :
Laboratoire d'Immunologie-Vaccinologie, Faculté de médecine vétérinaire, Université de Liège
Disciplines :
Microbiology
Author, co-author :
Zeippen, Caroline ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Vaccinologie vétérinaire
Language :
French
Title :
Etude de la transmission du gammaherpèsvirus murin 4: importance de la glycoprotéine gp150 et développement de stratégies antivirales basées sur l'utilisation du cidofovir
Alternative titles :
[en] Study of Murid Herpesvirus-4 transmission: Importance of glycoprotein gp150 and development of antiviral strategies based on the use of cidofovir
Defense date :
13 November 2017
Number of pages :
158
Institution :
ULiège - Université de Liège
Degree :
doctorat en sciences vétérinaires
Promotor :
Gillet, Laurent ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
President :
Charlier, Carole ; Université de Liège - ULiège > GIGA > GIGA Medical Genomics - Unit of Animal Genomics
Jury member :
Vanderplasschen, Alain ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Willems, Luc ; Université de Liège - ULiège > Département GxABT
Michiels, Thomas
Dubuisson, Jean
Gillet, Nicolas
Sadzot, Catherine ; Université de Liège - ULiège > Département des sciences de la vie > Virologie - Immunologie
Thiry, Etienne ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI)
Jacobs, Nathalie ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques
Name of the research project :
Study of the neutralization evasion by gammaherpesviruses in the context of transmission, development of original vaccination strategies
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.