Article (Scientific journals)
The Effects of Dietary N-3 and Antioxidant Supplementation on Erythrocyte Membrane Fatty Acid Composition and Fluidity in Exercising Horses
Portier, Karine; de Moffarts, Brieuc; Fellman, Nicole et al.
2006In Equine Veterinary Journal, 36, p. 279-284
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Keywords :
horse; exercise; erythrocyte; antioxidant; membrane fluidity; (n-3) PUFA
Abstract :
[en] REASONS FOR PERFORMING STUDY: Fatty acid supplementation could modulate erythrocyte membrane fluidity in horses at rest and during exercise, but information is lacking on the effect of exercise. OBJECTIVES: To assess the effect of exercise with, and without, an oral antioxidant supplementation enriched with n-3 fatty acids on erythrocyte membrane fluidity (EMF) and fatty acid composition in eventing horses. METHODS: Twelve healthy and regularly trained horses were divided randomly into 2 groups: group S received an oral antioxidant cocktail enriched in n-3 fatty acid (alphatocopherol, eicosapentaenoic acid [EPA] and docosahexaenoic acid [DHA]) whereas group P was placebo-treated. At the end of 4 weeks, all horses performed a standardised exercise test (ET) under field conditions. Venous blood was sampled before starting treatment (TO), immediately before (T1) as well as 15 min (T2) and 24 h (T3) after ET. Spin labelled (16-DOXYL-stearic acid) red blood cell membranes were characterised using the relaxation correlation time (Tc in inverse proportion to EMF). Fatty acid composition (%) of the membrane was determined by gas-liquid chromatography. RESULTS: Supplementation did not induce changes in EMF (T1 vs. TO) but significant changes in membrane composition were observed and there were increases in n-3 polyunsaturated fatty acid PUFA, n-3/n-6 ratio, and total n-3 fatty acids. Exercise (T2 vs. T1) induced a significant decrease of EMF in group P (Tc: +19%, P<0.05) and nonsignificant decrease in group S (Tc: +5%), whereas membrane fatty acid composition did not change in either group. During the recovery period (T3 vs. T2), EMF decreased significantly in group S (Tc: +29%, P<0.05) and nonsignificantly in group P (Tc: +18%) without any significant changes in fatty acid composition. CONCLUSION AND POTENTIAL RELEVANCE: An enriched oral antioxidant supplementation induced changes in membrane composition, which modulated the decrease in EMF induced by exercise. Long chain n-3 fatty acid supplementation might therefore be beneficial.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Veterinary medicine & animal health
Author, co-author :
Portier, Karine;  National of Veterinary School of Lyon, France > Equine department
de Moffarts, Brieuc;  Université de Liège - ULiège > Faculty of Veterinary Medicine - dept of Functional Sciences
Fellman, Nicole;  University of Auvergne, France > Faculty of Medicine, Laboratory of Sport Physiology and Biology
Kirschvink, Nathalie;  Université de Liège - ULiège > Faculty of Veterinary Medicine - dept of Functional Sciences
Motta, C.;  University of Auvergne, France > Faculty of Medicine, Laboratory of Sport Physiology and Biology
Letellierw, C.;  University of Auvergne, France > Faculty of Medicine, Laboratory of Sport Physiology and Biology
Ruelland, A.;  University of Auvergne, France > Faculty of Medicine, Laboratory of Sport Physiology and Biology
Van Erck, Emmanuelle ;  Université de Liège - ULiège > Faculty of Veterinary Medicine - dept of Functional Sciences
Lekeux, Pierre ;  Université de Liège - ULiège > Faculty of Veterinary Medicine - dept of Functional Sciences
Couder, Jean;  University of Auvergne, France > Faculty of Medicine, Laboratory of Sport Physiology and Biology
Language :
English
Title :
The Effects of Dietary N-3 and Antioxidant Supplementation on Erythrocyte Membrane Fatty Acid Composition and Fluidity in Exercising Horses
Publication date :
2006
Journal title :
Equine Veterinary Journal
ISSN :
0425-1644
eISSN :
2042-3306
Publisher :
Equine Veterinary Journal Ltd, Newmarket Suffolk, United Kingdom
Volume :
36
Pages :
279-284
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 09 September 2009

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