Reference : Neuro-functional correlates of the protective effects of exercise against cocaine sen...
Scientific congresses and symposiums : Paper published in a journal
Human health sciences : Radiology, nuclear medicine & imaging
http://hdl.handle.net/2268/215189
Neuro-functional correlates of the protective effects of exercise against cocaine sensitization and dopamine D2 receptors density: a [18F]Fallypride microPET study.
English
Becker, Guillaume mailto [Université de Liège - ULiège > > Centre de recherches du cyclotron >]
Lespine, Louis-Ferdinand mailto [Université de Liège - ULiège > Département de Psychologie > Neuroscience comportementale et psychopharmacologie expér. >]
Serrano Navacerrada, Maria Elisa mailto [Université de Liège - ULiège > > Centre de recherches du cyclotron >]
Bahri, Mohamed Ali mailto [Université de Liège - ULiège > > Centre de recherches du cyclotron >]
Luxen, André mailto [Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de chimie organique de synthèse >]
Lemaire, Christian mailto [Université de Liège - ULiège > > Centre de recherches du cyclotron >]
Tirelli, Ezio mailto [Université de Liège - ULiège > Département de Psychologie > Neuroscience comportementale et psychopharmacologie expér. >]
Plenevaux, Alain mailto [Université de Liège - ULiège > Département de chimie (sciences) > Département de chimie (sciences) >]
In press
Molecular Imaging and Biology
Springer
Yes (verified by ORBi)
No
International
1536-1632
1860-2002
New York
NY
World Molecular Imaging Congress - WMIC 2017
13 septembre 2017 - 16 spetembre 2017
World Molecular Imaging Society
Philadelphia
USA
[en] cocaine ; dopamine ; D2 ; fallypride ; microPET ; imaging ; preclinical ; physical exercice
[en] Preclinical studies suggest that free access to a running wheel can attenuate behavioural effects of addictive drugs such as psychomotor sensitization to cocaine in rodents. This phenomenon has an integral role in the process of drug addiction in craving and relapse (Steketee and Kalivas, 2011). Free wheel-running was recently shown to be efficacious at preventing the initiation or the long-term expression of psychomotor sensitization to cocaine in mice or rats (Diaz et al., 2013; Geuzaine and Tirelli, 2014). In the present study, we investigated the neuro-functional correlates of the protection against psychomotor sensitization to cocaine afforded by free wheel-running on dopaminergic neurotransmission, using microPET imaging with [18F]Fallypride, a Dopamine 2 receptor (D2R) antagonist.

Sixty-four 28-day-old female C57BL/6J mice were randomly assigned to one of the two housing conditions, defined by the presence or the absence of a running wheel in the cage over a 6-week pre-testing period. Since mice from the two types of housing received either saline (controls) or cocaine (8 mg/kg, i.p.) during testing (9 once-daily sessions to establish sensitization plus 1 single session to test its expression), a basic 2x2 randomized blocks design was generated (2-way ANOVA and planned comparisons; n=10). Experimentation lasted 85 days, with a 42-day period of pre-testing and a 3-week interval preceding the test for long-term expression of sensitization (LTES). All mice underwent a microPET (Focus 120, Siemens) the day after the LTES. The microPET protocol consisted of a 60 min. dynamic acquisition after the injection of 10 MBq of [18F]Fallypride in the tail vein.

Wheel-running strongly and significantly attenuated LTES (interaction) to cocaine (Cohen’s d=1.63; t(21)=3.75, p<.001) and cocaine-treated mice exhibited a clear-cut and significant increase (main effect) of the [18F]Fallypride BP (d=0.88, t(31)=2.45, p =.02). Wheel-running induced an overall moderate-sized decrease (main effect) of the [18F]Fallypride BP, but without achieving statistical significance (d=0.64, t(31)=1.79, p =.08). These findings suggest that LTES is associated with an increase of the [18F]Fallypride BP in the mouse striatum, probably reflecting an increase in postsynaptic D2R density in this region. Also, the protecting effect of free running on psychomotor sensitization goes together with a decrease in D2R density in the striatum of exercised mice. We intend to complement the present study with an identical experiment to reach a total number of 80 mice (n=20). This will confer to our study a sufficient power (80%) for the main effect of wheel-running exercise on [18F]Fallypride BP to be detected at an alpha-level of 5%. Finally, autoradiography studies, performed on the same mice with [18F]Fallypride, will strengthen our in vivo results.
Centre de Recherches du Cyclotron - CRC
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/215189

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
WMIC_2017_abstract_Becker_etal.pdfAuthor preprint256.11 kBRequest copy
Restricted access
WMIC_2017_Poster_Becker.pdfAuthor preprint970.35 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.