[en] Peels of four local Citrus species (Citrus sinensis L. Obsbeck, Citrus reticulata Blanco, Citrus maxima Burm. and Citrus aurantifolia christm.) grown in a same locality under the same climatic conditions were subjected to steam distillation. The essential oils and floral waters obtained were analyzed by gas chromatography coupled with a flame ionization detector (CPG-FID) and by gas chromatography coupled to a mass spectrometer (CPG-MS). The essential oils yields were ranged between 0.2 and 0.7 %. The qualitative and quantitative analyses show 74 compounds in all oils samples. The oils were largely dominated by hydrocarbon monoterpenes with limonene as major compound (91.5-94.0 %; 90.4-93.4 %; 77.7-93.7 % and 34.8-51.2 %) in oils from Citrus sinensis, Citrus reticulata, Citrus maxima and Citrus aurantifolia, respectively. In addition to limonene, oils of Citrus aurantifolia contained high amounts of β-pinene (6.8-14.4 %), pcymene (5.8-12.2 %), γ-terpinene (0.3 -7.4 %), neral (1.5-9.4 %), geraniol (1.9-12.7 %). Although presenting the same major compound (limonene), the oils had compositions that were qualitatively and quantitatively different. The enantiomeric analyses showed that the chiral major compounds were (R) - (+) - limonene, (S) - (-) – limonene, (1S) - (-) -β-pinene, (1R)-(+)-β-pinene and (S) - (+) - carvone. The hydrosols analyzed were
composed of oxygenated compounds and did not have the same major compound. A qualitative and quantitative difference in chemical composition was noted between the Hydrosols.
Fisher, K., Phillips, C., (2008). Potential antimicrobial uses of essential oils in food:is citrus the answer? Trends in Food Science & Technology, 19:156–164. doi:10.1016/j.tifs.2007.11.006
Baser, K.H.C., Buchbauer, G., (2010). Handbook of Essential oils Science, Technology, and Applications. Taylor and Francis Group, LLC, 949 p.
Lante, A., Tinello, F., (2015). Citrus hydrosols as useful by-products for tyrosinase inhibition. Innovative Food Science and Emerging Technologies. 27:154–159. doi:10.1016/j.ifset.2014.11.001
Dugo, G., Bonaccorsi, I., Sciarrone, D., Costa, R., Dugo, P., Mondello, L., Santi, L., Fakhry, H.A., (2011). Characterization of Oils from the Fruits, Leaves and Flowers of the Bitter Orange Tree. Journal of Essential Oil Research. 23(2):45–59.
Singh, P., Shukla, R., Prakash, B., Kumar, A., Singh, S., Mishra, P.K., Dubey, N.K., (2010). Chemical profile, antifungal, antiaflatoxigenic and antioxidant activity of Citrus maxima Burm. and Citrus sinensis (L.) Osbeck essential oils and their cyclic monoterpene, DL-limonene. Food and Chemical Toxicology. 48:1734–1740. doi:10.1016/j.fct.2010.04.001
Espina, L., Somolinos, M., Lorán, S., Conchello, P., García, D., Pagán, R., (2011). Chemical composition of commercial citrus fruit essential oils and evaluation of their antimicrobial activity acting alone or in combined processes. Food Control. 22:896–902. doi:10.1016/j.foodcont.2010.11.021
Padrayuttawat, A., Yoshizawa, T., Tamura, H., Tokunaga, T., (1997). Optical Isomers and Odor Thresholds of Volatile Constituents in Citrus sudachi. Food Science and Technology International, Tokyo. 3(4):402–408 doi:10.3136/fsti9596t9798.3.402
Labadie, C., Ginies, C., Guinebretiere, M.H., Renard, C.M.G.C., Cerutti, C., Carlin, F., (2015). Hydrosols of orange blossom (Citrus aurantium), and roseflower (Rosa damascena and Rosa centifolia) support the growth of a heterogeneous spoilage microbiota. Food Research International. 76:576–586. doi:10.1016/j.foodres.2015.07.014
Adams, R.P., (2001). Identification of Essential Oil Components by Gas Chromatography /Quadrupole Mass Spectroscopy. Allured Publishing, Carol Stream, IL, USA 2001, 455 p.
Joulain, D, Konig, W.A., (1998). The Atlas of Spectral Data of Sesquiterpene Hydrocarbons. EB-Verlag, Hamburg.
Kamal, G.M., Anwar, F., Hussain, A.I., Sarri, N., Ashraf, M.Y., (2011). Yield and chemical composition of Citrus essential oils as affected by drying pretreatment of peels. International Food Research Journal. 18(4):1275–1282.
Michaelakis, A, Papachristos, D, Kimbaris, A, Koliopoulos, G, Giatropoulos, A, Polissiou, M.G., (2009). Citrus essential oils and four enantiomeric pinenes against Culexpipiens (Diptera:Culicidae). Parasitol Res. 105:769–773. doi:10.1007/s00436-009-1452-7
Sawamura, M., Thiminhtu, N., Onishi, Y., Ogawa, E., Choi, H.S., (2004). Characteristic Odor Components of Citrus reticulata Blanco (Ponkan) Cold-pressed Oil. Bioscience, Biotechnology, and Biochemistry. 68(8):1690–1697. doi:10.1271/bbb.68.1690
Ahmad, M.M., Rehman, S.U., Iqbal, Z., Anjum, F.M., Sultan, J.I., (2006). Genetic variability to essential oil composition in four citrus fruit species. Pak. J. Bot. 38(2):319–324.
Bourgou, S., Rahali, F.Z., Ourghemmi, I., Tounsi, M.S., (2012). Changes of Peel Essential Oil Composition of Four Tunisian Citrus during Fruit Maturation. The Scientific World Journal. 1–10. doi:10.1100/2012/528593
Campolo, O., Malacrinò, A., Zappalà, L., Laudani, F., Chiera, E., Serra, D., Russo, M., Palmeri, V., (2014). Fumigant bioactivity of five Citrus essential oils against Tribolium confusum. Phytoparasitica. 42:223–233. doi:10.1007/s12600-013-0355-4
Dugo, G., Cotroneo, A., Bonaccorsi, I., Trozzi, A., (2010). Composition of the Volatile Fraction of Citrus Peel Oils. Citrus Oils, 1 in Dugo G., Mondello L., (Eds.). Citrus oils:composition, advanced analytical techniques, contaminants, and biological activity. CRC Press.
Tranchida, P.Q., Bonaccorsi, I., Dugo, P., Mondello, L., Dugo, G., (2012). Analysis of Citrus essential oils:state of the art and future perspectives. A review. Flavour Fragr. J. 27:98–123. doi:10.1002/ffj.2089
Karlberg, A.T., Magnusson, K., Nilsson, U., (1992). Air oxidation of d-limonene (the citrus solvent) creates potent allergens. Contact Dermatitis. (26):332–340. doi:10.1111/j.1600-0536.1992.tb00129.x
Monsef-Esfahani, H.R., Amanzade, Y., Alhani, Z., Hajimehdipour, H., Faramarzi, M.A., (2004). GC/MS Analysis of Citrus aurantium L. Hydrolate and its Comparison with the Commercial Samples. Iranian Journal of Pharmaceutical Research. 3:177–179.
