Reference : Mesenchymal Stromal Cells Accelerate Epithelial Tight Junction Assembly via the AMP-A...
Scientific journals : Article
Human health sciences : Urology & nephrology
http://hdl.handle.net/2268/214338
Mesenchymal Stromal Cells Accelerate Epithelial Tight Junction Assembly via the AMP-Activated Protein Kinase Pathway, Independently of Liver Kinase B1
English
Rowart, Pascal mailto [Université de Liège - ULiège > Département des sciences cliniques > Néphrologie >]
ERPICUM, Pauline [Centre Hospitalier Universitaire de Liège - CHU > > Service de néphrologie >]
KRZESINSKI, Jean-Marie mailto [Centre Hospitalier Universitaire de Liège - CHU > > Service de néphrologie >]
Sebbagh, Michaël mailto [INSERM: Marseille, Provence-Alpes-Côte d'Azu, France > > > >]
JOURET, François mailto [Centre Hospitalier Universitaire de Liège - CHU > > Service de néphrologie >]
11-Jul-2017
Stem Cells International
Hindawi Publishing Corporation
Yes (verified by ORBi)
International
1687-966X
1687-9678
[en] Background. Mesenchymal stromal cells (MSC) are fibroblast-like multipotent cells capable of tissue-repair properties. Given the essentiality of tight junctions (TJ) in epithelial integrity, we hypothesized that MSC modulate TJ formation, via the AMP-activated kinase (AMPK) pathway. Liver kinase-β1 (LKB1) and Ca2+-calmodulin-dependent protein kinase kinase (CaMKK) represent the main kinases that activate AMPK. Methods. The in vitro Ca2+ switch from 5 μM to 1.8 mM was performed using epithelial Madin-Darby canine kidney (MDCK) cells cultured alone or cocultured with rat bone marrow-derived MSC or preexposed to MSC-conditioned medium. TJ assembly was measured by assessing ZO-1 relocation to cell-cell contacts. Experiments were conducted using MDCK stably expressing short-hairpin-RNA (shRNA) against LKB1 or luciferase (LUC, as controls). Compound STO-609 (50 μM) was used as CaMKK inhibitor. Results. Following Ca2+ switch, ZO-1 relocation and phosphorylation/activation of AMPK were significantly higher in MDCK/MSC compared to MDCK. No difference in AMPK phosphorylation was observed between LKB1-shRNA and Luc-shRNA MDCK following Ca2+ switch. Conversely, incubation with STO-609 prior to Ca2+ switch prevented AMPK phosphorylation and ZO-1 relocation. MSC-conditioned medium slightly but significantly increased AMPK activation and accelerated TJ-associated distribution of ZO-1 post Ca2+ switch in comparison to regular medium. Conclusions. MSC modulate the assembly of epithelial TJ, via the CaMKK/AMPK pathway independently of LKB1.
http://hdl.handle.net/2268/214338
10.1155/2017/9717353
https://www.hindawi.com/journals/sci/2017/9717353/abs/

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