[en] Apoptosis is a tightly regulated cellular process that plays an essential role in the development, aging, cancer biology, immune response, and pathogenesis of various diseases. Herein, we report a new SERS sensing strategy for in vitro sensitive detection of early apoptotic cells. The principle of this method is to in situ synthesize silver nanoparticles (AgNPs) on the phosphatidylserine (PS) of the apoptotic cell membrane during the early apoptosis, which enables distinguishing normal and apoptotic cells. The total assay time of the presented method is only 10 min, thus being faster, cheaper and simpler than current techniques for the detection of apoptosis. The intrinsic mechanism was verified by different approaches based on externalized phosphatidylserine. In addition, the detection process is real-time and label-free; i.e., the intrinsic SERS spectra from the cellular membrane are directly employed for apoptosis real-time detection, which avoids using additional chemical or biological reagents as external signal indicators. Therefore, our SERS approach may serve as a potentially practical tool for sensitive and real-time detection of early cell apoptosis, complementing the state-of-the-art strategies, e.g. flow cytometry. While further investigation is required to better understand the intrinsic mechanism of the in situ coating method, the current results may provide another choice for real-time detection of early apoptosis.
Research Center/Unit :
epartment of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou, Guangdong Province 510632, China Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan Province 610041, China ollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan Schools of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang Province 310058, China Chair for Analytical Chemistry, Technische Universität München, Marchioninistr. 17, D-81377 Munich, Germany Comprehensive Pneumology Center (CPC), Institute of Lung Biology and Disease, Helmholtz Zentrum München, Neuherberg, Germany
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Huang, Yang ; Université de Liège - ULiège > Doct. sc. bioméd. & pharma. (paysage)
Language :
English
Title :
Early Apoptosis Real-Time Detection by Label-free SERS Based on Externalized Phosphatidylserine
Publication date :
03 May 2016
Journal title :
Analyst
ISSN :
0003-2654
eISSN :
1364-5528
Publisher :
Royal Society of Chemistry, Cambridge, United Kingdom
Volume :
141
Issue :
2016
Pages :
4293-4298
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Natural Science Foundation of China (21505053 and 21405138) China Postdoctoral Science Foundation (2014M550328) cience and Technology Planning Project of Guangdong Province, China (2015A030401045, 2016A030310089) Guangzhou Key Laboratory of Innovative Chemical Drug Research in Cardiac-cerebral Vascular Disease (151800010) Fundamental Research Funds for the Central Universities, Jinan University (21615324 and 21615711)
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Bibliography
Elmore, S., (2007) Toxicol. Pathol., 35, pp. 495-516
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