Triterpenoid saponins; Supercritical fluid chromatography; Traditional Chinese medicines
Abstract :
[en] Triterpenoid saponins (TSs) are the most important components of some traditional Chinese medicines (TCMs) and have exhibited valuable pharmacological properties. In this study, a rapid and efficient method was developed for the separation of kudinosides, stauntosides and ginsenosides using supercritical fluid chromatography coupled with single quadrupole mass spectrometry (SFC-MS). The separation conditions for the selected TSs were carefully optimized after the initial screening of eight stationary phases. The best compromise for all compounds in terms of chromatographic performance and MS sensitivity was obtained when water (5–10%) and formic acid (0.05%) were added to the supercritical carbon dioxide/MeOH mobile phase. Beside the composition of the mobile phase, the nature of the make-up solvent for interfacing SFC with MS was also evaluated. Compared to reversed phase liquid chromatography, the SFC approach showed higher resolution and shorter running time. The developed SFC-MS methods were successfully applied to the separation and identification of TSs present in Ilex latifolia Thunb., Panax quinquefolius L. and Panax ginseng C.A. Meyer. These results suggest that this SFC-MS approach could be employed as a useful tool for the quality assessment of natural products containing TSs as active components
Research Center/Unit :
Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, Jinan University Guangzhou 510632, China Laboratory of Analytical Pharmaceutical Chemistry, Department of Pharmaceutical Sciences, University of Liege, CHU B36, B-4000 Liege, Belgium
Disciplines :
Physical, chemical, mathematical & earth Sciences: Multidisciplinary, general & others
Author, co-author :
Huang, Yang ; Université de Liège - ULiège > Doct. sc. bioméd. & pharma. (paysage)
Language :
English
Title :
Fast separation of triterpenoid saponins using supercritical fluidchromatography coupled with single quadrupole mass spectrometry
Publication date :
03 January 2016
Journal title :
Journal of Pharmaceutical and Biomedical Analysis
ISSN :
0731-7085
eISSN :
1873-264X
Publisher :
Elsevier, Amsterdam, Netherlands
Volume :
121
Issue :
2016
Pages :
22-29
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
National Natural Science Foundation of China (Grant: 81303204, 21505053) Natural Science Foundation of Guangdong Province of China (2015A030401045) China Postdoctoral Science Foundation (Grant: 2013M531907)
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