[en] Capillary electrophoresis (CE) for HPV-VLP quantitation is a very interesting alternative technique compared to those currently used in viral analysis, such as SDS-PAGE, Western blot or protein assay that are destructive and semi-quantitative or non specific. In this study, the quantitative performance of the CE method was evaluated. A main issue in virus quantitation is the absence of reference material. Therefore, the concentration of a HPV16-VLP sample produced in the laboratory was determined using ELISA with Gardasil(R), after adjuvant dissolution, as reference material and conformational H16.V5 antibody. HPV16-VLP concentration was found to influence particles electrophoretic mobility until a plateau was reached for concentrations
Research Center/Unit :
Centre Interfacultaire de Recherche du Médicament - CIRM
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Bettonville, Virginie ; Université de Liège > Département de pharmacie > Analyse des médicaments
Nicol, Jerome T. J.
Furst, Tania
Thelen, Nicolas ; Université de Liège > Département des sciences de la vie > GIGA-R : Biologie cellulaire
Piel, Géraldine ; Université de Liège > Département de pharmacie > Pharmacie galénique
Thiry, Marc ; Université de Liège > Département des sciences de la vie > GIGA-R : Biologie cellulaire
Fillet, Marianne ; Université de Liège > Département de pharmacie > Analyse des médicaments
Jacobs, Nathalie ; Université de Liège > Département des sciences biomédicales et précliniques > Virologie
Servais, Anne-Catherine ; Université de Liège > Département de pharmacie > Analyse des médicaments
Language :
English
Title :
Quantitation and biospecific identification of virus-like particles of human papillomavirus by capillary electrophoresis.
Publication date :
2017
Journal title :
Talanta
ISSN :
0039-9140
eISSN :
1873-3573
Publisher :
Elsevier, Netherlands
Volume :
175
Pages :
325-330
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique Fonds Léon Fredericq
Commentary :
Copyright (c) 2017 Elsevier B.V. All rights reserved.
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