Article (Scientific journals)
In Vivo Imaging of Flavoprotein Fluorescence During Hypoxia Reveals the Importance of Direct Arterial Oxygen Supply to Cerebral Cortex Tissue.
Chisholm, K. I.; Ida, Keila; Davies, A. L. et al.
2016In Advances in Experimental Medicine and Biology, 876, p. 233-9
Peer Reviewed verified by ORBi
 

Files


Full Text
oxygen transport tissue.pdf
Publisher postprint (14.68 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Animals; Cell Hypoxia; Cerebral Cortex/metabolism; Flavoproteins/analysis; Fluorescence; Mice; Mice, Inbred C57BL; Mitochondria/physiology; Oxygen/metabolism; Brain; Confocal microscope; Mitochondria; Oxygen; Vasculature
Abstract :
[en] Live imaging of mitochondrial function is crucial to understand the important role played by these organelles in a wide range of diseases. The mitochondrial redox potential is a particularly informative measure of mitochondrial function, and can be monitored using the endogenous green fluorescence of oxidized mitochondrial flavoproteins. Here, we have observed flavoprotein fluorescence in the exposed murine cerebral cortex in vivo using confocal imaging; the mitochondrial origin of the signal was confirmed using agents known to manipulate mitochondrial redox potential. The effects of cerebral oxygenation on flavoprotein fluorescence were determined by manipulating the inspired oxygen concentration. We report that flavoprotein fluorescence is sensitive to reductions in cortical oxygenation, such that reductions in inspired oxygen resulted in loss of flavoprotein fluorescence with the exception of a preserved 'halo' of signal in periarterial regions. The findings are consistent with reports that arteries play an important role in supplying oxygen directly to tissue in the cerebral cortex, maintaining mitochondrial function.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Chisholm, K. I.
Ida, Keila ;  Université de Liège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Anesthésiologie et réanimation vétérinaires
Davies, A. L.
Papkovsky, D. B.
Singer, M.
Dyson, A.
Tachtsidis, I.
Duchen, M. R.
Smith, K. J.
Language :
English
Title :
In Vivo Imaging of Flavoprotein Fluorescence During Hypoxia Reveals the Importance of Direct Arterial Oxygen Supply to Cerebral Cortex Tissue.
Publication date :
2016
Journal title :
Advances in Experimental Medicine and Biology
ISSN :
0065-2598
eISSN :
2214-8019
Publisher :
Springer, New York, United States - New York
Volume :
876
Pages :
233-9
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 July 2017

Statistics


Number of views
44 (1 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
12
Scopus citations®
without self-citations
7
OpenCitations
 
9

Bibliography


Similar publications



Contact ORBi