Article (Scientific journals)
An Update on Aptamer-Based Multiplex System Approaches for the Detection of Common Foodborne Pathogens
Mukama, Omar; Rwibasira, Peter; Sinumvayo, Jean Paul et al.
2017In Food Analytical Methods
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Keywords :
Aptamers, Modified SELEX,; Foodborne pathogens; Food safety
Abstract :
[en] Food borne ailments constitute a public health challenge and pose an incredible economic burden in healthcare system around the globe. This dilemma has urged authorities and other entities working in field of food quality control and supply chain to play a pivotal role in ensuring food safety. Analytical strategies have been developed using numerous systematic evolution of ligands by exponential enrichment (SELEX) methods to assure food safety. High-affinity and high-sensitivity ssDNA and RNA aptamers against pathogens have emerged as a novel strategy, as compared to the more resource-demanding and complicated biochemical test-based approaches. Thus, this review aims to focus on some methods used in the selection of specific bare, modified, and conjugated aptamers and on the further analysis of selected aptamers using flow cytometer or post-SELEX modifications for enhanced detection of frequently diagnosed foodborne bacteria such as Bacillus sp., Campylobacter jejuni, Escherichia sp., Salmonella sp., Staphylococcus aureus, Shigella sp., Listeria monocytogenes, and Streptococcus pyogenes and/or targeting their cell components towards attaining fast, sensitive, and selective methods for the detection of pathogens in food(s) or other sources.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Mukama, Omar;  Jiangnan University > Laboratory of Carbohydrate Chemistry and Biotechnology
Rwibasira, Peter  ;  Université de Liège - ULiège > Form. doct. sc. (biol. orga. & écol. - paysage)
Sinumvayo, Jean Paul;  Jiangnan University > Laboratory of Carbohydrate Chemistry and Biotechnology
Wang, Zhouping;  Jiangnan University > Synergetic Innovation Center of Food Safety and Nutrition
Language :
English
Title :
An Update on Aptamer-Based Multiplex System Approaches for the Detection of Common Foodborne Pathogens
Publication date :
28 January 2017
Journal title :
Food Analytical Methods
ISSN :
1936-9751
eISSN :
1936-976X
Publisher :
Springer, New York, United States - New York
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 17 June 2017

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