Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

[en] The field of assisted reproduction has been developed to treat infertility in women, companion animals, and endangered species. In the horse, assisted reproduction also allows for the production of embryos from high performers without interrupting their sports career and contributes to an increase in the number of foals from mares of high genetic value. The present manuscript describes the procedures used for collecting immature and mature oocytes from horse ovaries using ovum pick-up (OPU). These oocytes were then used to investigate the incidence of aneuploidy by adapting a protocol previously developed in mice. Specifically, the chromosomes and the centromeres of metaphase II (MII) oocytes were fluorescently labeled and counted on sequential focal plans after confocal laser microscope scanning. This analysis revealed a higher incidence in the aneuploidy rate when immature oocytes were collected from the follicles and matured in vitro compared to in vivo. Immunostaining for tubulin and the acetylated form of histone four at specific lysine residues also revealed differences in the morphology of the meiotic spindle and in the global pattern of histone acetylation. Finally, the expression of mRNAs coding for histone deacetylases (HDACs) and acetyl-transferases (HATs) was investigated by reverse transcription and quantitative-PCR (q-PCR). No differences in the relative expression of transcripts were observed between in vitro and in vivo matured oocytes. In agreement with a general silencing of the transcriptional activity during oocyte maturation, the analysis of the total transcript amount can only reveal mRNA stability or degradation. Therefore, these findings indicate that other translational and post-translational regulations might be affected. Overall, the present study describes an experimental approach to morphologically and biochemically characterize the horse oocyte, a cell type that is extremely challenging to study due to low sample availability. However, it can expand our knowledge on the reproductive biology and infertility in monovulatory species.

Disciplines :

Anatomie (cytologie, histologie, embryologie...) & physiologie

Auteur, co-auteur :

Franciosi, Federica; Università degli Studi di Milano > Department of Health > animal science and food safety

Tessario, Irene; Università degli Studi di Milano > Department of Health > Animal science and food safety

Dalbies-Tran, Rozenn

Douet, Cecile; Institut Scientifique de Recherche Agronomique - INRA > PRC

Reigner, Fabrice; Institut Scientifique de Recherche Agronomique - INRA > UEPAO

Deleuze, Stefan ; Université de Liège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Obstét. et path. de la reprod. des anim. de comp. et équidés

Papiller, Pascal; Institut Scientifique de Recherche Agronomique - INRA > PRC

Maclea, Ileana; University of agricultural science and veterinary medicine, Cluj-Napoca

Lodde, Valentina; Università degli Studi di Milano > Department of health > Animal science and food safety

Luciano, Alberto; Università degli Studi di Milano > Department of health > Animal science and food safety

Goudet, Ghylene; Institut Scientifique de Recherche Agronomique - INRA > PRC

Langue du document :

Anglais

Titre :

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Date de publication/diffusion :

2017

Titre du périodique :

Journal of Visualized Experiments

eISSN :

1940-087X

Maison d'édition :

MYJoVE Corporation, Boston, Etats-Unis - Massachusetts

Volume/Tome :

123

Fascicule/Saison :

e55242

Peer reviewed :

Peer reviewed vérifié par ORBi

Disponible sur ORBi :

depuis le 12 juin 2017

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283 (dont 3 ULiège)

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