[en] Drought is one of the major abiotic factors affecting growth and productivity of plants by imposing certain morphological, physiological and biochemical changes at different growth stages. The objective of this work is to study key morphological, physiological and biochemical response of faba bean (Vicia faba L. var. 'minor') to soil water deficit stress and to assess the contribution of genetic factors in improving faba bean tolerance to water deficit. Plant of 11 faba bean cultivars were grown in the greenhouse and subjected to three levels of water deficit (90, 50 and 30% of field capacity (FC)) in a simple randomized design for 20 days. Water deficit effects on plant growth, relative water content (RWC), gaz exchange, chlorophyll a (Chla) and Chlorophyll b (Chlb) content, osmoprotectant accumulations (such as proline and soluble sugars), antioxydant enzyme activities and grain yield were determined. Soil water deficit stress reduced growth and affected physiological parameters, especially antioxidant enzyme activities. Water deficit also increased proline, soluble sugars and protein contents. The studied cultivars significantly differed in their responses to water deficit stress. Photosynthetic parameters were less affected in the 'Hara' cultivar. Furthermore, this cultivar produced the highest value of grain yield at 30% FC, and showed higher antioxidant enzyme activities (CAT, GPX and APX), osmoprotectant accumulations, Chlb and RWC. The 'Hara' cultivar was found to be more tolerant to water deficit stress than the other cultivars. Our methodology can be used for assessing the response of faba bean genetic resources to soil water deficit. The identified tolerant cultivar can be utilized as a source for water stress tolerance in faba bean breeding programs aimed at improving drought tolerance.
Disciplines :
Agriculture & agronomy
Author, co-author :
Abid, Ghassen; University of Tunis El Manar, 901B, TN-2050 Hammam-Lif, Tunisia > Biotechnology Center of Borj Cedria > Laboratory of Legumes
Hessini, Kamel; Taif Unversity, Faculty of Science, Al-Haweiah, Taif, Kingdom of Saudi Arabia > Biology Department
Aouida, Marwa; University of Tunis El Manar, 901B, TN-2050 Hammam-Lif, Tunisia > Biotechnology Center of Borj Cedria > Laboratory of Legumes
Aroua, Ibtissem; University of Tunis El Manar, 901B, TN-2050 Hammam-Lif, Tunisia > Biotechnology Center of Borj Cedria > Laboratory of Legumes
Baudoin, Jean-Pierre ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Phytotechnie tropicale et horticulture
Muhovski, Yordan; Walloon Agricultural Research Centre, Chaussée de Charleroi, 234, BE-5030 Gembloux, Belgium > Department of Life Sciences > Breeding and Biodiversity Unit
Mergeai, Guy ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Phytotechnie tropicale et horticulture
Sassi, Khaled; Unversity of Carthage, National Agronomy Institute of Tunisia (INAT), avenue Charles Nicolle, 43, TN-1082 Tunis-Mahrajène, Tunisia > Department of Agronomy and Plant Biotechnology
Machraoui, Myriam; University of Carthage, National Agronomy Institute of Tunisia (INAT), avenue Charles Nicolle, 43, TN-1082 Tunis-Mahrajène, Tunisia > Department of Agronomy and Plant Biotechnology
Souissi, Fatma; University of Tunis El Manar, 901 B, TN-2050 Hammam-Lif, Tunisia > Biotechnology Center of Borj Cedria > Laboratory of Legumes
Jebara, Moez; University of Tunis El Manar, 901B, TN-2050 Hammam-Lif, Tunisia > Biotechnology Center of Borj Cedria > Laboratory of Legumes
Language :
English
Title :
Agro-physiological and biochemical responses of faba bean (Vicia faba L. var. 'minor') genotypes to water deficit stress
Alternative titles :
[fr] Comportement agro-physiologique et biochimique de différents génotypes de féverole (Vicia fava L. var. 'minor') soumis au déficit hydrique
Publication date :
2017
Journal title :
Biotechnologie, Agronomie, Société et Environnement
ISSN :
1370-6233
eISSN :
1780-4507
Publisher :
Presses Agronomiques de Gembloux, Gembloux, Belgium
Abdellatif K.F., El Absawy S.A. & Zakaria A.M., 2012. Drought stress tolerance of faba bean as studied by morphological traits and seed storage protein pattern. J. Plant Stud., 1, 47-54.
Abid G. et al., 2015. Genetic relationship and diversity analysis of faba bean (Vicia faba L. var. minor) genetic resources using morphological and microsatellite molecular markers. Plant Mol. Biol. Rep., 33, 1755-1767.
Ahmadi A. & Siosemardeh A., 2005. Investigation on the physiological basis of grain yield and drought resistance in wheat: leaf photosynthetic rate, stomatal conductance, and non-stomatal limitations. Int. J. Agric. Biol., 7, 807-811.
Alghamdi S.S. et al., 2015. Physiological and molecular characterization of faba bean (Vicia faba L.) genotypes for adaptation to drought stress. J. Agron. Crop Sci., 201, 401-409.
Ali M.B.M. et al., 2016. Association analyses to genetically improve drought and freezing tolerance of faba bean (Vicia faba L.). Crop Sci., 56, 1036-1048.
Amede T. & Schubert S., 2003. Mechanisms of drought resistance in seed legumes. I.-Osmotic adjustment. Ethiop. J. Sci., 26, 37-46.
Ammar M.H. et al., 2014. Physiological and yield responses of faba bean (Vicia faba L.) to drought stress in managed and open field environments. J. Agron. Crop Sci., 201, 280-287.
Anjum S.A. et al., 2011. Morphological, physiological and biochemical responses of plants to drought stress. Afr. J. Agric. Res., 6, 2026-2032.
Arnon D.I., 1949. Copper enzymes in isolated chloroplasts, polyphenol oxidase in Beta vulgaris. Plant Physiol., 24, 1-15.
Beauchamp C. & Fridovich I., 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem., 44, 276-287.
Benešová M. et al., 2012. The physiology and proteomics of drought tolerance in maize: early stomatal closure as a cause of lower tolerance to short-term dehydration? PLoS One, 7, 1-17.
Bradford M.M., 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 722, 248-254.
Dhindsa R.S., Plumb-Dhindsa P. & Thorpe T.A., 1981. Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J. Exp. Bot., 32, 93-101.
Emam Y., Shekoofa A., Salehi F. & Jalali A.H., 2010. Water stress effects on two common bean cultivars with contrasting growth habits. Am.-Eurasian J. Agric. Environ. Sci., 9, 495-499.
Farooq M. et al., 2009. Plant drought stress: effects, mechanisms and management. Agron. Sustain. Dev., 29, 185-212.
Fischer R.A. et al., 1998. Wheat yield progress associated with higher stomatal conductance and photosynthetic rate, and cooler canopies. Crop Sci., 38, 1467-1475.
Galmes J., Flexas J., Save R. & Medrano H., 2007. Water relations and stomatal characteristics of Mediterranean plants with different growth forms and leaf habits: responses to water stress and recovery. Plant Soil, 290, 139-155.
Ghaderi N. & Siosemardeh A., 2011. Response to drought stress of two strawberry cultivars (cv. Kurdistan and Selva). Hortic. Environ. Biotechnol., 52, 6-12.
Harb A., Awada D. & Samarah N., 2015. Gene expression and activity of antioxidant enzymes in barley (Hordeum vulgare L.) under controlled severe drought. J. Plant Interact., 10, 109-116.
Hasheminasab H., Assad M.T., Aliakbari A. & Sahhafi S.R., 2012. Influence of drought stress on oxidative damage and antioxidant defense system in tolerant and susceptible wheat genotypes. J. Agric. Sci., 4, 8-30.
Hayat S. et al., 2012. Role of proline under changing environments. Plant Signaling Behav., 7, 1456-1466.
Hoagland D.R. & Arnon D.I., 1950. The water culture method for growing plant without soil. Circular 347. Berkeley, CA, USA: University of California, The College of Agriculture, California Agricultural Experiment Station.
Hossaina M.M. et al., 2014. Differences between soybean genotypes in physiological response to sequential soil drying and rewetting. Crop J., 2, 366-380.
Jaleel C.A. et al., 2009. Drought stress in plants: a review on morphological characteristics and pigments composition. Int. J. Agric. Biol., 11, 100-105.
Khalafallah A.A., Tawfik K.M. & Abd El-Gawad Z.A., 2008. Tolerance of seven faba bean varieties to drought and salt stresses. Res. J. Agric. Biol. Sci., 4, 175-186.
Khan H.R., Link W., Hocking T.J. & Stoddard F.L., 2007. Evaluation of physiological traits for improving drought tolerance in faba bean (Vicia faba L.). Plant Soil, 292, 205-217.
Kharrat M. & Ouchari H., 2011. Faba bean status and prospects in Tunisia. Grain Legumes, 56, 11-12.
Link W. et al., 1999. Genotypic variation for drought tolerance in Vicia faba. Plant Breed., 118, 477-483.
Lopes M.S. et al., 2012. The yield correlations of selectable physiological traits in a population of advanced spring wheat lines grown in warm and drought environments. Field Crops Res., 128, 129-136.
Lopez-Bellido F.J., Lopez-Bellido L. & Lopez-Bellido R.J., 2005. Competition, growth and yield of faba bean (Vicia faba L.). Eur. J. Agron., 23, 359-378.
Lu C. & Zhang J., 1998. Change in photosynthesis II function during senescence of wheat leaves. Physiol. Plant., 104, 239-247.
Maalouf F., 2011. Faba bean improvement at ICARDA: constraints and challenges. Grain Legumes, 56, 13-14.
Mafakheri A. et al., 2010. Effect of drought stress on yield, proline and chlorophyll contents in three chickpea cultivars. Aust. J. Crop Sci., 4, 580-585.
Maritimi T.K. et al., 2015. Physiological and biochemical response of tea [Camellia sinensis (L.) O. Kuntze] to water-deficit stress. J. Hortic. Sci. Biotechnol., 90, 395-400.
Mohamad Zabawi A.G. & Dennett M.D.D., 2010. Responses of faba bean (Vicia faba) to different levels of plant available water: I. Phenology, growth and biomass partitioning. J. Trop. Agric. Food Sci., 38, 11-19.
Mohammadkhani N. & Heidari R., 2008. Drought-induced accumulation of soluble sugars and proline in two maize varieties. World Appl. Sci. J., 3, 448-453.
Mwanamwenge J., Loss S.P., Siddique K.H.M. & Cocks P.S., 1999. Effect of water stress during floral initiation, flowering and podding on the growth and yield of faba bean (Vicia faba L.). Eur. J. Agron., 11, 1-11.
Nagahatenna D.S.K., Langridge P. & Whitford R., 2015. Tetrapyrrole-based drought stress signaling. Plant Biotechnol. J., 13, 447-459.
Nakano Y. & Asada K., 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol., 22, 867-880.
Nazarli H. & Faraji F., 2011. Response of proline, soluble sugars and antioxidant enzymes in wheat (Triticum aestivum L.) to different irrigation regimes in green house condition. Cercetări Agronomice Moldova, 148, 27-33.
Plewa M.J., Smith S.R. & Wanger E.D., 1991. Diethyldithiocarbamate suppresses the plant activation of aromatic amines into mutagens by inhibiting tobacco cell peroxidase. Mutat. Res., 247, 57-64.
Ratnayaka H.H. & Kincaid D., 2005. Gas exchange and leaf ultrastructure of tinnevelly senna, Cassia angustifolia, under drought and nitrogen stress. Crop Sci., 45, 840-847.
Richardson C., Yan S. & Vestal C.G., 2015. Oxidative stress, bone marrow failure, and genome instability in hematopoietic stem cells. Int. J. Mol. Sci., 16, 2366-2385.
Roohi E., Tahmasebi-Sarvestani Z., Modarres-Sanavy S.A.M. & Siosemardeh A., 2013. Comparative study on the effet of soil water stress on photosynthesis function of triticale, bread wheat, and barley. J. Agric. Sci. Technol., 15, 215-228.
Rosa M. et al., 2009. Soluble sugars-metabolism, sensing and abiotic stress. Plant Signal. Behav., 4, 388-393.
Rozrokh M., Sabaghpour S.H., Armin M. & Asgharipour M., 2012. The effects of drought stress on some biochemical traits in twenty genotypes of chickpea. Eur. J. Exp. Biol., 2, 1980-1987.
Saeidi M. & Abdoli M., 2015. Effect of drought stress during grain filling on yield and its components, gas exchange variables, and some physiological traits of wheat cultivars. J. Agric. Sci. Technol., 17, 885-898.
Shao H.B. et al., 2008. Higher plant antioxidants and redox signaling under environmental stresses. C.R. Biol., 331, 433-441.
Sharma P., Jha A.B., Dubey R.S. & Pessarakli M., 2012. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J. Bot., 2012, ID 217037.
Sheela Devi S.P. & Sujatha B., 2014. Drought-induced accumulation of soluble sugars and proline in two pigeon pea (Cajanus Cajan L. Millsp.) cultivars. Int. J. Innovative Res. Dev., 3, 302-306.
Siddiqui M.H. et al., 2015. Response of different genotypes of faba bean plant to drought stress. Int. J. Mol. Sci., 16, 10214-10227.
Tavakkoli E., Rengasamy P. & McDonald G.K., 2010. High concentrations of Na+ and Cl-ions in soil solution have simultaneous detrimental effects on growth of faba bean under salinity stress. J. Exp. Bot., 61, 4449-4459.
Türkan I., Bor M., Ozdemir F. & Koca H., 2005. Differential response of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris subjected to polyethylene glycol mediated water stress. Plant Sci., 168, 223-231.
Uzilday B. et al., 2012. Comparaison of ROS formation and antioxidant enzymes in Cleome gynandra (C4) and Cleome spinosa (C3) under drought stress. Plant Sci., 182, 59-70.
Wu G.Q., Zhang L.N. & Wang Y.Y., 2012. Response of growth and antioxidant enzymes to osmotic stress in two different wheat (Triticum aestivum L.) cultivars seedlings. Plant Soil Environ., 58, 534-539.
Yamasaki S. & Dillenburg L.R., 1999. Measurement of leaf relative water content in Araucaria angustifolia. Rev. Bras. Fisiol. Veg., 11, 69-75.
Yooyongwech S., Cha-um S. & Supaibulwatana K., 2012. Proline related genes expression and physiological changes in Indica rice response to water-deficit stress. Plant Omics, 5(6), 597-603.
Zarafshar M. et al., 2014. Morphological, physiological and biochemical responses to soil water deficit in seedlings of three populations of wild pear tree (Pyrus boisseriana). Biotechnol. Agron. Soc. Environ., 18, 353-366.
Zhou S. et al., 2013. How should we model plant responses to drought? An analysis of stomatal and non-stomatal responses to water stress. Agric. For. Meteorol., 182, 204-214.
Ziadi Backouchi I., Aouida M. & Jebara M., 2015. Drought stress response in Tunisian populations of faba bean (Vicia faba L.). J. Plant Biol. Res., 4, 55-72.
