Article (Scientific journals)
Susceptibility of piglets to enterotoxigenic Escherichia coli is not related to the expression of MUC13 and MUC20
Schroyen, Martine; Stinckens, A.; Verhelst, R. et al.
2012In Animal Genetics, 43 (3), p. 324-327
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Keywords :
ETEC-F4ac; MUC13; MUC20; Escherichia coli infection; Animals; Antigens, Neoplasm; Disease Susceptibility; Enterotoxigenic Escherichia coli; Escherichia coli Infections; Fatty Acid-Binding Proteins; Gene Expression; Gene Expression Regulation; Intestinal Mucosa; Intestine, Small; Lectins, C-Type; Mucins; Reverse Transcriptase Polymerase Chain Reaction; Swine; Swine Diseases; Tumor Markers, Biological; Animalia; Bacteria (microorganisms); Escherichia coli; Suidae
Abstract :
[en] Enterotoxigenic Escherichia coli (ETEC) is one of the most frequently isolated enteropathogens in production animals, especially pigs and calves. Economically, the swine industry is by far the most affected by infections with ETEC because of mortality, morbidity and decreased growth rate of newborn and early-weaned piglets. After ingestion by the animal, these bacteria attach themselves to specific receptors on the small intestinal epithelium by means of proteinaceous surface appendages, the fimbriae. The F4 fimbriae, which attach to the F4 receptor, are the most studied. The aim of our study was to investigate gene expression in the small intestine of piglets of MUC13 and MUC20 in relation to animals with a different treatment towards or a different reaction on ETEC-F4ac by means of quantitative reverse transcription chain reaction (qRT/PCR). MUC13 and MUC20 are positional candidate genes for this F4ac receptor and are located in the region on SSC13q41 that segregates with the susceptibility to ETEC-F4ac. The condition of the small intestine is crucial when examining expression differences between different samples. Therefore, the expression of two genes, fatty-acid binding protein 2, intestinal (FABP2) and pancreatitis-associated protein (PAP), now known as regenerating islet-derived 3 alpha (REG3A) in the small intestine was simultaneously checked. FABP2, a standard for epithelial content, reflects the state of damage, whereas REG3A is a measure for inflammation in the small intestine. The four different substudies presented here suggest that expression of MUC13 and MUC20 is not related to the susceptibility of piglets to ETEC-F4ac. © 2011 The Authors, Animal Genetics © 2011 Stichting International Foundation for Animal Genetics.
Disciplines :
Genetics & genetic processes
Author, co-author :
Schroyen, Martine  ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Zootechnie
Stinckens, A.;  Livestock Genetics, Department Biosystems, KULeuven, Kasteelpark Arenberg 30, 3001 Heverlee, Belgium
Verhelst, R.;  Nutrition and Health, Department Biosystems, KULeuven, Kasteelpark Arenberg 30, 3001 Heverlee, Belgium
Geens, M.;  Nutrition and Health, Department Biosystems, KULeuven, Kasteelpark Arenberg 30, 3001 Heverlee, Belgium
Cox, E.;  Department of Virology, Parasitology and Immunology, UGent, Salisburylaan 133, 9820 Merelbeke, Belgium
Niewold, T.;  Nutrition and Health, Department Biosystems, KULeuven, Kasteelpark Arenberg 30, 3001 Heverlee, Belgium
Buys, N.;  Livestock Genetics, Department Biosystems, KULeuven, Kasteelpark Arenberg 30, 3001 Heverlee, Belgium
Title :
Susceptibility of piglets to enterotoxigenic Escherichia coli is not related to the expression of MUC13 and MUC20
Publication date :
2012
Journal title :
Animal Genetics
ISSN :
0268-9146
eISSN :
1365-2052
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
43
Issue :
3
Pages :
324-327
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 24 April 2017

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