Pig; Dietary fibre; Bodyweight; In vitro fermentation; Gas-test technique; Sugar beet pulp; Porcin; Fibre alimentaire; Poids corporel; Fermentation in vitro; Technique du gaz-test; Betterave sucrière
Abstract :
[en] Two experiments were undertaken to investigate the influence of (1) pig bodyweight and (2) dietary fibre content of the diet on the in vitro gas production of sugar beet pulp fibre using faecal inoculum. In the first experiment, inocula prepared from young pigs (Y; 16–50 kg), growing pigs (G; 62–93 kg) and sows (S; 216–240 kg) were compared. Sugar beet pulp, hydrolysed in vitro with pepsin and then pancreatin, was used as the fermentation substrate. The cumulated gas productions over 144 h were modelled and the kinetics parameters compared. Lag times (Y: 4.6 h; G: 6.4 h; S: 9.2 h) and halftimes to asymptote (Y: 14.7 h; G: 15.9 h; S: 20.8 h) increased with pig bodyweight (P<0.001) and the fractional degradation rates of the substrate differed between the pig categories (Y: 0.110 h−1; G: 0.115 h−1; S: 0.100 h−1; P<0.001). The final gas productionwas not affected (P=0.10) by the inoculum source. In the second experiment hydrolysed sugar beet pulp was fermented with four inocula prepared from pigs fed diets differing in their total and soluble dietary fibre contents, i.e. low fibre diet rich in soluble fibre (LOW-S) or in insoluble fibre (LOW-I) or high fibre diet rich in soluble fibre (HIGHS) or in insoluble fibre (HIGH-I). The total and the soluble dietary fibres influenced the kinetics of gas production. The presence of soluble fibres decreased the lag times, whatever the total dietaryfibre content (2.7 h for LOW-S versus 3.5 h for LOW-I, 4.0 h for HIGH-S versus 4.4 h for HIGH-I; P<0.001). The half-times to asymptote were higher with the low fibre diets (P<0.001) and, for similar total dietary fibre contents, they were lower when the proportion of soluble fibres increased (LOW-S: 9.9 h; LOW-I: 11.4 h; HIGH-S: 8.9 h; HIGH-I: 10.1 h; P<0.001). The fractional degradation rates of the substrate were the highest with the fibre-rich diet containing a high proportion of soluble fibres (0.158 h−1; P<0.001). In conclusion, the bodyweight of the faeces donors and the dietary fibre composition of the pig diet influence the in vitro fermentation kinetics of hydrolysed sugar beet pulp, but not the final gas production.
Disciplines :
Animal production & animal husbandry Agriculture & agronomy
Awati A., Konstantinov S.R., Williams B.A., Akkermans A.D.L., Bosch M.W., Smidt H., and Martin Verstegen W.A. Effect of substrate adaptation on the microbial fermentation and microbial composition of faecal microbiota of weaning piglets studied in vitro. J. Sci. Food Agric. 85 (2005) 1765-1772
Bach Knudsen K.E., Jensen B.B., Andersen J.O., and Hansen I. Gastrointestinal implications in pigs of wheat and oat fractions. 2. Microbial activity in the gastrointestinal tract. Br. J. Nutr. 65 (1991) 233-248
Barry J.L., Hoebler C., Macfarlane G.T., Macfarlane S., Mathers J.C., Reed K.A., Mortensen P.B., Nordgaard I., Rowland I.R., and Rumney C.J. Estimation of the fermentability of dietary fibre in vitro: a European interlaboratory study. Br. J. Nutr. 74 (1995) 303-322
Bauer E., Williams B.A., Bosch M.W., Voigt C., Mosenthin R., and Verstegen M.W.A. Microbial activities of faeces from unweaned and adult pigs, in relation to selected fermentable carbohydrates. Anim. Sci. 73 (2001) 313-322
Bauer E., Williams B.A., Bosch M.W., Voigt C., Mosenthin R., and Verstegen M.W.A. Differences in microbial activity of digesta from three sections of the porcine large intestine according to in vitro fermentation of carbohydrate-rich substrates. J. Sci. Food Agric. 84 (2004) 2097-2104
Bauer E., Williams B.A., Voigt C., Mosenthin R., and Verstegen M.W.A. Impact of mammalian enzyme pretreatment on the fermentability of carbohydrate-rich feedstuffs. J. Sci. Food Agric. 83 (2003) 207-214
Bindelle J., Buldgen A., Boudry C., and Leterme P. Effect of inoculum and pepsin-pancreatin hydrolysis on fibre fermentation measured by the gas production technique in pigs. Anim. Feed Sci. Technol. 132 (2007) 111-122
Boisen S., and Fernández J.A. Prediction of the total tract digestibility of energy in substrates and pigs diets by in vitro analyses. Anim. Feed Sci. Technol. 68 (1997) 228-277
Dagnelie P. Théorie et Méthodes Statistiques. second ed. vol. 2 (1975), Les Presses Agronomiques de Gembloux, Gembloux, Belgium
Dierick N.A., Vervaeke I.J., Demeyer D.I., and Decuypere J.A. Approach to the energetic importance of fibre digestion in pigs. I. Importance of fermentation in the overall energy supply. Anim. Feed Sci. Technol. 23 (1989) 141-167
Faisant N., Planchot V., Kozlowski F., Pacouret M.P., Colonna P., and Champ M. Resistant starch determination adapted to products containing high level of resistant starch. Sci. Aliment. 15 (1995) 83-89
France J., Dhanoa M.S., Theodorou M.K., Lister S.J., Davies D.R., and Isac D. A model to interpret gas accumulation profiles associated with in vitro degradation of ruminant feeds. J. Theor. Biol. 163 (1993) 99-111
Freire J.P.B., Guerreiro A.J.G., Cunha L.F., and Aumaitre A. Effect of dietay fibre source on total tract digestibility, caecum volatile fatty acids and digestive transit time in the weaned piglet. Anim. Feed Sci. Technol. 87 (2000) 71-83
Jensen B.B., and Jørgensen H. Effect of dietary fiber on microbial activity and microbial gas production in various regions of the gastrointestinal tract of pigs. Appl. Environ. Microbiol. 60 (1994) 1897-1904
Katouli M., Lund A., Wallgren P., Kühn I., Söderlind O., and Möllby R. Metabolic fingerprinting and fermentative capacity of the intestinal flora of pigs during pre- and post weaning periods. J. Appl. Microbiol. 83 (1997) 145-147
Lee S.C., Prosky L., and De Vries J.W. Determination of total, soluble, and insoluble dietary fiber in foods-enzymatic-gravimetric method MES-TRIS buffer: collaborative study. J. AOAC Int. 75 (1992) 395-415
Le Goff G., Noblet J., and Cherbut C. Intrinsic ability of the faecal microbial flora to ferment dietary fibre at different growth stages of pigs. Livest. Prod. Sci. 81 (2003) 75-87
Le Goff G., van Milgen J., and Noblet J. Influence of dietary fibre on digestive utilization and rate of passage in growing pigs, finishing pigs and adult sows. Anim. Sci. 74 (2002) 503-515
Leterme P., Londoño A.M., Estrada F., Souffrant W., and Buldgen A. Chemical composition, nutritive value and voluntary intake of tropical tree foliage and cocoyam in pigs. J. Sci. Food Agric. 85 (2005) 1725-1735
Leterme P., Botero M., Londoño A.M., Bindelle J., and Buldgen A. Nutritive value of tropical tree leaf meals in adult sows. Anim. Sci. 82 (2006) 175-184
Lifschitz C.H. Colonic short-chain fatty acids in infants and children. In: Cummings J.H., Rombeau J.L., and Sakata T. (Eds). Physiological and Clinical Aspects of Short-Chain Fatty Acids (1995), Cambridge University Press, Cambridge, United Kingdom
McBurney M.I., and Thompson L.U. Effect of human faecal donor on in vitro fermentation variables. Scand. J. Gastroenterol. 24 (1989) 359-367
McBurney M.I., Horvath P.J., Jeraci J.L., and Van Soest P.J. Effect of in vitro fermentation using human faecal inoculum on the water-holding capacity of dietary fibre. Br. J. Nutr. 53 (1985) 17-24
Menke K.H., and Steingass H. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Dev. 28 (1988) 7-55
Merry R.J., and MacAllan A.B. A comparison of the chemical composition of mixed bacteria harvested from the liquid and the solid fractions of rumen digesta. Br. J. Nutr. 50 (1983) 701-709
Noblet J., and Le Goff G. Effect of dietary fibre on the energy value of feeds for pigs. Anim. Feed Sci. Technol. 90 (2001) 35-52
Theodorou M.K., Williams B.A., Dhanoa M.S., McAllan A.B., and France J. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminat feeds. Anim. Feed Sci. Technol. 48 (1994) 185-197
Varel V.H., and Yen J.T. Microbial perspective on fiber utilization by swine. J. Anim. Sci. 75 (1997) 2715-2722
Wang J.F., Zhu Y.H., Li D.F., Wang Z., and Jensen B.B. Effect of type and level of dietary fibre and starch on ileal and faecal microbiological activity and short-chain fatty acid concentrations in growing pigs. Anim. Sci. 78 (2004) 109-117
Williams B.A., Verstegen M.W.A., and Tamminga S. Fermentation in the large intestine of single-stomached animals and its relationship to animal health. Nutr. Res. Rev. 14 (2001) 207-227
Wenk C. The role of dietary fibre in the digestive physiology of the pig. Anim. Feed Sci. Technol. 90 (2001) 21-33
