[en] Yersinia ruckeri, the causative agent of enteric red mouth disease (ERM), is a widely studied pathogen in disease models using rainbow trout. This infection model, mostly based on intraperitoneally injection or bath immersion challenges, has an impact on both components (innate and adaptive) of the fish immune system. Although there has been much attention in studying its host-pathogen interactions, there is still a lack of knowledge regarding the impact of a cohabitation challenge. To tackle this we used a newly established non-lethal sampling method (by withdrawing a small amount of blood) in rainbow trout which allowed the individual immune monitoring before (non-infected) and after infection with Yersinia ruckeri either by intraperitoneal (i.p.) injection or by cohabitation (cohab). A range of key immune genes were monitored during the infection by real-time PCR, and results were compared between the two infection routes. Results indicated that inflammatory (IL-1beta1 and IL-8) cytokines and certain antimicrobial peptides (cathelicidins) revealed a different pattern of expression between the two infected groups (i.p. vs cohab), in comparison to adaptive immune cytokines (IL-22, IFN-gamma and IL-4/13A) and beta-defensins. This suggests a different involvement of distinct immune markers according to the infection model, and the importance of using a cohabitation challenge as a more natural disease model that likely simulates what would occur in the environment.
Fernández, L., Méndez, J., Guijarro, J.A., Molecular virulence mechanisms of the fish pathogen Yersinia ruckeri (2007) Vet. Microbiol., 125 (1–2), pp. 1-10
Tobback, E., Decostere, A., Hermans, K., Haesebrouck, F., Chiers, K., Yersinia ruckeri infections in salmonid fish (2007) J. Fish. Dis., 30 (5), pp. 257-268
Kumar, G., Menanteau-Ledouble, S., Saleh, M., El-Matbouli, M., Yersinia ruckeri, the causative agent of enteric redmouth disease in fish (2015) Vet Res., 46, p. 103
Ohtani, M., Villumsen, K.R., Strøm, H.K., Raida, M.K., 3D Visualization of the initial Yersinia ruckeri infection route in rainbow trout (Oncorhynchus mykiss) by optical projection tomography (2014) PLoS One, 9 (2), p. e89672
Wang, T., Bird, S., Koussounadis, A., Holland, J.W., Carrington, A., Zou, J., Secombes, C.J., Identification of a novel IL-1 cytokine family member in teleost fish (2009) J. Immunol., 183, pp. 962-974
Raida, M.K., Buchmann, K., Development of adaptive immunity in rainbow trout, Oncorhynchus mykiss (Walbaum) surviving an infection with Yersinia ruckeri (2008) Fish. Shellfish Immunol., 25 (5), pp. 533-541
Monte, M.M., Zou, J., Wang, T., Carrington, A., Secombes, C.J., Cloning, expression analysis and bioactivity studies of rainbow trout (Oncorhynchus mykiss) Interleukin-22 (2011) Cytokine, 55 (1), pp. 62-73
Monte, M.M., Wang, T., Holland, J., Zou, J., Secombes, C.J., Cloning and characterization of rainbow trout interleukin-17A/F2 (IL-17A/F2) and IL-17 receptor A: expression during infection and bioactivity of recombinant IL-17A/F2 (2013) Infect. Immun., 81 (1), pp. 340-353
Raida, M.K., Holten-Andersen, L., Buchmann, K., Association between Yersinia ruckeri infection, cytokine expression and survival in rainbow trout (Oncorhynchus mykiss) (2011) Fish. Shellfish Immunol., 30 (60), pp. 1257-1264
Collet, B., Urquhart, K., Monte, M., Collins, C., Garcia Perez, S., Secombes, C.J., Hall, M., Individual monitoring of immune response in Atlantic salmon Salmo salar following experimental infection with Infectious Salmon Anaemia Virus (ISAV) (2015) PLoS One, 10 (9), p. e0137767
Thorsen, B.K., Enger, O., Norland, S., Hoff, K.A., Long-term starvation survival of Yersinia ruckeri at different salinities studied by microscopical and flow cytometric methods (1992) Appl. Environ. Microbiol., 58, pp. 1624-1628
Nordmo, R., Ramstad, A., Comparison of different challenge methods to evaluate the efficacy of furunculosis vaccines in Atlantic salmon, Salmo salar L (1997) J. Fish Dis., 20, pp. 119-126
Chettri, J.K., Skov, J., Jaafar, R.M., Krossøy, B., Kania, P.W., Dalsgaard, I., Buchmann, K., Comparative evaluation of infection methods and environmental factors on the challenge success: Aeromonas salmonicida infection in vaccinated rainbow trout (2015) Fish. Shellfish Immunol., 44 (2), pp. 485-495
Robertson, P.A.W., O'Dowd, C., Burrells, C., Williams, P., Austin, B., Use of Carnobacterium sp. as a probiotic for Atlantic salmon (Salmo salar L.) and rainbow trout (Oncorhynchus mykiss, Walbaum) (2000) Aquaculture, 185, pp. 235-243
Urquhart, K., Collins, C., Monte, M., Sokolowska, J., Secombes, C., Collet, B., Individual measurement of gene expression in blood cells from rainbow trout Oncorhynchus mykiss (Walbaum) (2016) J. Exp. Appl. Anim. Sci., 2 (1), pp. 1-9
Wang, T., Díaz-Rosales, M.S., Secombes, C.J., Cloning of a novel interleukin (IL)-20- like gene in rainbow trout Oncorhynchus mykiss gives an insight into the evolution of the IL-10 family (2010) Dev. Comp. Immunol., 34, pp. 158-167
Menanteau-Ledouble, S., Krauss, I., Santos, G., Fibi, S., Weber, B., El-Matbouli, M., Effect of a phytogenic feed additive on the susceptibility of Oncorhynchus mykiss to Aeromonas salmonicida (2015) Dis. Aquat. Organ, 115 (1), pp. 57-66
Harun, N.O., Wang, T., Secombes, C.J., Gene expression profiling in naïve and vaccinated rainbow trout after Yersinia ruckeri infection: insights into the mechanisms of protection seen in vaccinated fish (2011) Vaccine, 29 (26), pp. 4388-4399
Gibello, A., Blanco, M.M., Moreno, M.A., Cutuli, M.T., Domenech, A., Dominguez, L., Development of a PCR assay for detection of Yersinia ruckeri in tissues of inoculated and naturally infected trout (1999) Appl. Environ. Microbiol., 65, pp. 346-350
Wang, T., Huang, W., Costa, M.M., Martin, S.A.M., Secombes, C.J., Two copies of the genes encoding the subunits of putative interleukin (IL)-4/IL-13 receptors, IL- 4Rα, IL-13Rα1 and IL-13Rα2, have been identified in rainbow trout (Oncorhynchus mykiss) and have complex patterns of expression and modulation (2011) Immunogenetics, 63, pp. 235-253
Altinok, I., Grizzle, J.M., Liu, Z., Detection of Yersinia ruckeri in rainbow trout blood by use of the polymerase chain reaction (2001) Dis. Aquat. Organ, 44 (1), pp. 29-34
Nordmo, R., Strengths and weaknesses of different challenge methods (1997) Dev. Biol. Stand, 90, pp. 303-309
Alcorn, S., Murray, A.L., Pascho, R.J., Varney, J., A cohabitation challenge to compare the efficacies of vaccines for bacterial kidney disease (BKD) in Chinook salmon Oncorhynchus tshawytscha (2005) Dis. Aquat. Organ, 63, pp. 151-160
Hadidi, S., Glenney GWm Welch, T.J., Silverstein, J.T., Wiens, G.D., Spleen size predicts resistance of rainbow trout to Flavobacterium psychrophilum challenge (2008) J. Immunol., 180 (6), pp. 4156-4165
Liu, S., Vallejo, R.L., Palti, Y., Gao, G., Marancik, D.P., Hernandez, A.G., Wiens, G.D., Identification of single nucleotide polymorphism markers associated with bacterial cold water disease resistance and spleen size in rainbow trout. Front. Genet. 6: 298
Afonso, A., Lousada, S., Silva, J., Ellis, A.E., Silva, M.T., Neutrophil and macrophage responses to inflammation in the peritoneal cavity of rainbow trout Oncorhynchus mykiss. A light and electron microscopic cytochemical study (1998) Dis. Aquat. Organ, 34 (1), pp. 27-37
Hong, S., Zou, J., Collet, B., Bols, N.B., Secombes, C.J., Analysis and characterisation of IL-1β processing in rainbow trout, Oncorhynchus mykiss (2004) Fish. Shellfish Immunol., 16 (3), pp. 453-459
Harun, N.O., Zou, J., Zhang, Y.A., Nie, P., Secombes, C.J., The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8 (2008) Dev. Comp. Immunol., 32 (6), pp. 673-681
Chettri, J.K., Raida, M.K., Kania, O.W., Buchmann, K., Differential immune response of rainbow trout (Oncorhynchus mykiss) at early developmental stages (larvae and fry) against the bacterial pathogen Yersinia ruckeri (2012) Dev. Comp. Immunol., 36 (2), pp. 463-474
Chang, C.I., Zhang, Y.A., Zou, J., Nie, O., Secombes, C.J., Two cathelicidin genes are present in both rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar) (2006) Antimicrob. Agents Chemother., 50 (1), pp. 185-195
Casadei, E., Wang, T., Zou, J., González Vecino, J.L., Wadsworth, S., Secombes, C.J., Characterization of three novel beta-defensin antimicrobial peptides in rainbow trout (Oncorhynchus mykiss) (2009) Mol. Immunol., 46, pp. 3358-3366
Bridle, A., Nosworthy, E., Polinski, M., Nowak, B., Evidence of an antimicrobial-immunomodulatory role of Atlantic salmon cathelicidins during infection with Yersinia ruckeri (2011) PLoS One, 6 (8), p. e23417