Cloning and characterization of rainbow trout interleukin-17A/F2 (IL-17A/F2) and IL-17 receptor A: expression during infection and bioactivity of recombinant IL-17A/F2.

Mira Monte, Milena; Wang, Tiehui; Holland, Jason W.; Zou, Jun; Secombes, Christopher J.

doi:10.1128/IAI.00599-12

Article (Scientific journals)

Cloning and characterization of rainbow trout interleukin-17A/F2 (IL-17A/F2) and IL-17 receptor A: expression during infection and bioactivity of recombinant IL-17A/F2.

Mira Monte, Milena; Wang, Tiehui; Holland, Jason W. et al.

2013 • In Infection and Immunity, 81 (1), p. 340-53

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/205344

DOI
10.1128/IAI.00599-12

PubMed
23147036

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Keywords :

Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular/methods; Down-Regulation/genetics/immunology; Escherichia coli/genetics; Immunity, Innate/genetics/immunology; Interleukin-17/genetics/immunology/metabolism; Interleukin-6/genetics/immunology/metabolism; Interleukin-8/genetics/immunology/metabolism; Molecular Sequence Data; Oncorhynchus mykiss/genetics/immunology/metabolism; Receptors, Interleukin-17/genetics/immunology/metabolism; Recombinant Proteins/genetics/immunology

Abstract :

[en] Lower vertebrates have been found to possess genes that have similar homology to both interleukin (IL)-17A and IL-17F, which have been termed IL-17A/F. In fish species, several of these genes can be present, but, to date, very little is known about their functional activity. This article describes the discovery and sequence analysis of a rainbow trout (Oncorhynchus mykiss) IL-17A/F2 molecule and an IL-17RA receptor. In addition, the bioactivity of the trout IL-17A/F2 is investigated for the first time in any species. The predicted IL-17A/F2 and IL-17RA proteins consist of 146 and 966 amino acids (aa), respectively, with both molecules containing conserved family motifs. Expression analysis revealed high constitutive expression of trout IL-17A/F2 in mucosal tissues from healthy fish, suggesting a potential role in mucosal immunity. When the modulation of IL-17A/F2 and IL-17RA in vitro was analyzed, it was observed that the two molecules were similarly affected. The expression of IL-17A/F2 was also induced in head kidney during bacterial, parasitic, and viral infections, revealing a possible function in defense against such pathogens. However, downregulation of IL-17RA was seen in some tissues and infections. The recombinant IL-17A/F2 protein was produced in Escherichia coli and was found to affect the expression of an antimicrobial peptide and the proinflammatory cytokines IL-6 and IL-8 in splenocytes. Consistent with mammalian IL-17 homologues, our expression and bioactivity results imply that trout IL-17A/F2 plays an important role in promoting inflammatory and host innate immune responses directed against different pathogen groups.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Mira Monte, Milena ; Université de Liège > Immunologie et vaccinologie

Wang, Tiehui

Holland, Jason W.

Zou, Jun

Secombes, Christopher J.

Language :

English

Title :

Cloning and characterization of rainbow trout interleukin-17A/F2 (IL-17A/F2) and IL-17 receptor A: expression during infection and bioactivity of recombinant IL-17A/F2.

Publication date :

2013

Journal title :

Infection and Immunity

ISSN :

0019-9567

eISSN :

1098-5522

Publisher :

American Society for Microbiology, Washington, United States - District of Columbia

Volume :

Issue :

Pages :

340-53

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 16 January 2017

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