Article (Scientific journals)
Molecular characterisation of four class 2 cytokine receptor family members in rainbow trout, Oncorhynchus mykiss.
Mira Monte, Milena; Wang, Tiehui; Collet, Bertrand et al.
2015In Developmental and Comparative Immunology, 48 (1), p. 43-54
Peer Reviewed verified by ORBi
 

Files


Full Text
Monte et al, 2015.pdf
Publisher postprint (570.55 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Amino Acid Sequence; Animals; Base Sequence; Cell Line; Cloning, Molecular; Fish Diseases/immunology/virology; Gene Expression Regulation; Interleukin-10/genetics; Molecular Sequence Data; Oncorhynchus mykiss/genetics/immunology; Primary Cell Culture; Protein Structure, Tertiary; Receptors, Cytokine/biosynthesis/genetics; Sequence Analysis, DNA; Cell lines; Class 2 cytokine receptor family (CRF2); Head kidney leucocytes; In vitro stimulation; Oncorhynchus mykiss; Viral infection; Antimicrobial peptides; Cohabitation; Cytokines; Intraperitoneal injection; Non-lethal sampling; Yersinia ruckeri
Abstract :
[en] The interleukin (IL)-10 cytokine family includes IL-10, IL-19, IL-20, IL-22, IL-24, IL-26 and the lambda/type III interferons. They are highly pleiotropic and mediate a variety of activities, including immune suppression and antibacterial immunity. To exert their functions they signal through a heterodimeric receptor composed of a subunit with a long intracellular domain (R1 type receptors; IL-10R1, IL-20R1 or IL-22R1) and a subunit with a short intracellular domain (R2 type receptors; IL-10R2 or IL-20R2). In this study we report the identification of three R1 type receptors (named IL-10R1/CRFB7, IL-20R1a/CRFB8a and IL-20R1b/CRFB8b) and one R2 type receptor (named IL-10R2/CRFB4) in rainbow trout. The nomenclature of the receptors was supported by homology analysis, conserved motifs and phylogenetic tree analysis, confirming they belong to the piscine class 2 cytokine receptor family. For instance, they all displayed the presence of characteristic features, such as conserved fibronectin type-III domains. Expression analysis in tissues collected from healthy fish revealed different patterns of expression for each receptor, suggesting their potential involvement in different types of immune responses. When studying the modulation of the genes in cell lines and primary cultures, a greater effect was observed in the cell lines, where the expression of most receptors was affected by incubation with microbial mimics (LPS and PolyI:C) or the pro-inflammatory cytokine rIFN-gamma. In addition, expression of the four receptors was modulated by viral infection, suggesting a potential involvement of such receptors and their ligands in antiviral defence.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Mira Monte, Milena ;  Université de Liège > Immunologie et vaccinologie
Wang, Tiehui
Collet, Bertrand
Zou, Jun
Secombes, Chris J.
Collet, Bertrand
Language :
English
Title :
Molecular characterisation of four class 2 cytokine receptor family members in rainbow trout, Oncorhynchus mykiss.
Publication date :
2015
Journal title :
Developmental and Comparative Immunology
ISSN :
0145-305X
Publisher :
Elsevier, United Kingdom
Volume :
48
Issue :
1
Pages :
43-54
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2014 Elsevier Ltd. All rights reserved.
Available on ORBi :
since 16 January 2017

Statistics


Number of views
37 (5 by ULiège)
Number of downloads
1 (1 by ULiège)

Scopus citations®
 
14
Scopus citations®
without self-citations
14
OpenCitations
 
14

Bibliography


Similar publications



Contact ORBi