Article (Scientific journals)
Use of primary and secondary polyvinylamines for efficient gene transfection
Dréan, Mathilde; Debuigne, Antoine; Gonçalves, Christine et al.
2017In Biomacromolecules, 18 (2), p. 440-441
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Keywords :
biomaterial; nanomedicine; gene therapy
Abstract :
[en] Gene transfection with polymeric carrier remains a challenge, particularly high transfection levels combined with low toxicity are hard to achieve. We herein revisit polyvinylamines, an old and neglected family of cationic polymers. They can be readily obtained by controlled hydrolysis of polyvinylamides prepared through (controlled) radical polymerization. A series of tailor-made and well-defined polyvinylamines bearing primary amino groups, and poly(N- methylvinylamine) bearing secondary amines, were evaluated for the transfection of cells with pDNA as a function of their molar mass, molar mass distribution and degree of deacetylation. Unexpected high transfection levels, in combination with low cytotoxicity were recorded for both series. Surprisingly, a great impact of the molar mass was observed for the primary amine polyvinylamine series, whereas the results were mostly independent on molar mass or dispersity for the polymer bearing secondary amine. It was further established that a certain percentage of acetamide groups increased the transfection level, while maintaining low cytotoxicity. These results highlight for the first time the real potential of polyvinylamines as gene carriers, and make these polymers very attractive for further development in gene therapy.
Research center :
Center for Education and Research on Macromolecules (CERM)
CESAM - Complex and Entangled Systems from Atoms to Materials - ULiège
Disciplines :
Materials science & engineering
Chemistry
Author, co-author :
Dréan, Mathilde;  University of Liège - ULiège > Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM)
Debuigne, Antoine  ;  University of Liège - ULiège > Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM)
Gonçalves, Christine;  Centre de Biophysique Moléculaire, CNRS, Orléans, France
Jérôme, Christine  ;  University of Liège - ULiège > Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM)
Midoux, Patrick;  Centre de Biophysique Moléculaire, CNRS, Orléans, France
Rieger, Jutta ;  Sorbonne University, University Paris 6, CNRS, Institut Parisien de Chimie Moléculaire, Team “Chimie des Polymères” (LCP), Paris, France
Guégan, Philippe;  Sorbonne University, University Paris 6, CNRS, Institut Parisien de Chimie Moléculaire, Team “Chimie des Polymères” (LCP), Paris, France
Language :
English
Title :
Use of primary and secondary polyvinylamines for efficient gene transfection
Publication date :
13 February 2017
Journal title :
Biomacromolecules
ISSN :
1525-7797
eISSN :
1526-4602
Publisher :
American Chemical Society, Washington, United States - District of Columbia
Volume :
18
Issue :
2
Pages :
440-441
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
The International Doctoral School IDS-Funmat
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique
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