Membrane Interactions of Natural Cyclic Lipodepsipeptides of the Viscosin Group

[en] Many Pseudomonas spp. produce cyclic lipodepsipeptides (CLPs), which, besides their role in biological functions such as motility, biofilm formation and interspecies interactions, are antimicrobial. It has been established that interaction with the cellular membrane is central to the mode of action of CLPs. In this work, we focus on the CLPs of the so-called viscosin group, aiming to assess the impact of the main structural variations observed within this group on both the antimicrobial activity and the interaction with model membranes. The antimicrobial activity of viscosin, viscosinamide A, WLIP and pseudodesmin A were all tested on a broad panel of mainly Gram-positive bacteria. Their capacity to permeabilize or fuse PG/PE/cardiolipin model membrane vesicles is assessed using fluorescent probes. We find that the Glu2/Gln2 structural variation within the viscosin group is the main factor that influences both the membrane permeabilization properties and the minimum inhibitory concentration of bacterial growth, while the configuration of the Leu5 residue has no apparent effect. The CLPmembrane interactions were further evaluated using CD and FT-IR spectroscopy on model membranes consisting of PG/PE/cardiolipin or POPC with or without cholesterol. In contrast to previous studies, we observe no conformational change upon membrane insertion. The CLPs interact both with the polar heads and aliphatic tails of model membrane systems, altering bilayer fluidity, while cholesterol reduces CLP insertion depth

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Geudens, Niels ^✱

Nasir, Mehmet Nail ^✱; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie générale et organique

Crowet, Jean-Marc ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Biophysique moléc. aux interfaces

Raaijmakers, Jos

Fehér

Coenye, Tom

Martins, José

Lins, Laurence ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Biophysique moléc. aux interfaces

Sinnaeve, Davy ^✱

Deleu, Magali ^✱; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Biophysique moléc. aux interfaces

^✱ These authors have contributed equally to this work.

Language :

English

Title :

Membrane Interactions of Natural Cyclic Lipodepsipeptides of the Viscosin Group

Publication date :

2017

Journal title :

Biochimica et Biophysica Acta. Biomembranes

ISSN :

0005-2736

Publisher :

Elsevier Science

Volume :

1859

Issue :

Pages :

331-339

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://www.sciencedirect.com/science/article/pii/S0005273616304060

Name of the research project :

ARC- FIELD

Available on ORBi :

since 05 January 2017

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