Increased expression of bacterial amoA during an N2O emission peak in an agricultural field

Theodorakopoulos, Nicolas; Lognoul, Margaux; Degrune, Florine; Broux, François; Regaert, Donat; Muys, Céline; Heinesch, Bernard; Bodson, Bernard; Aubinet, Marc; Vandenbol, Micheline

doi:10.1016/j.agee.2016.12.002

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Increased expression of bacterial amoA during an N2O emission peak in an agricultural field

Theodorakopoulos, Nicolas; Lognoul, Margaux; Degrune, Florine et al.

2017 • In Agriculture, Ecosystems and Environment, 236, p. 212-220

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https://hdl.handle.net/2268/204240

DOI
10.1016/j.agee.2016.12.002

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Keywords :

Agricultural soil; Quantitative PCR; Nitrous oxide; amoA gene; Denitrification; Nitrification; Active bacterial diversity

Abstract :

[en] Levels of N-cycle gene transcripts (nirK, nirS, nosZ, amoA) were measured during an N2O emission peak in an agricultural soil. Automated dynamic closed chambers were used to monitor an N2O emission peak on a maize crop after a natural rainfall. The peak occurred rapidly after the rainfall began. Spatial and temporal variability in N2O emission was observed between chambers. An analysis of N-cycle gene transcript levels revealed an increase in bacterial amoA gene transcripts (but not in archaeal amoA transcripts), correlating strongly with N2O emission. This suggests the involvement of nitrification enzymes, despite a high water-filled pore space (80%). Reverse transcription of bacterial 16S rRNA followed by partial sequencing of the resulting cDNAs revealed few rainfall-induced changes in the potentially active bacterial community, and notably no significant change in the relative abundance of 16S rRNAs from the nitrifier genus Nitrosospira. Expression of the amoA gene appears as a possible proxy for monitoring the N2O emission peak. To our knowledge, this is the first experiment to evaluate the expression of N-cycle genes during an N2O emission peak on an agricultural field.

Disciplines :

Microbiology

Author, co-author :

Theodorakopoulos, Nicolas ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbiologie et génomique

Lognoul, Margaux ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Phytotechnie des régions tempérées

Degrune, Florine ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbiologie et génomique

Broux, François ; Université de Liège > Ingénierie des biosystèmes (Biose) > Echanges Ecosystèmes - Atmosphère

Regaert, Donat

Muys, Céline

Heinesch, Bernard ; Université de Liège > Ingénierie des biosystèmes (Biose) > Echanges Ecosystèmes - Atmosphère

Bodson, Bernard ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Phytotechnie des régions tempérées

Aubinet, Marc ; Université de Liège > Ingénierie des biosystèmes (Biose) > Echanges Ecosystèmes - Atmosphère

Vandenbol, Micheline ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbiologie et génomique

Language :

English

Title :

Increased expression of bacterial amoA during an N2O emission peak in an agricultural field

Publication date :

2017

Journal title :

Agriculture, Ecosystems and Environment

ISSN :

0167-8809

eISSN :

1873-2305

Publisher :

Elsevier Science, Amsterdam, Netherlands

Volume :

236

Pages :

212-220

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 12 December 2016

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