Neurohypophysial peptides stimulate the phosphorylation of pre-T cell focal adhesion kinases

[en] Thymic oxytocin (OT) behaves as a cryptocrine signal targeted at the outer surface of thymic epithelial cell plasma membrane from where OT is able to interact with neurohypophysial peptide receptors expressed by pre-T cells. Immature T cells bear a receptor of the V1 subtype, while OT receptors are predominantly expressed by cytotoxic CD8+ lymphocytes. In both T cell types, neurohypophysial peptide receptors transduce OT via the phosphoinositide pathway. Protein tyrosine phosphorylation is an early event of T cell activation. Western blots of murine pre-T cells (RL12-NP line) proteins probed with anti-phosphotyrosine (PY-20) revealed a great number of proteins the phosphorylation of which increased either with OT or vasopressin treatment. Two were immunoprecipitated with anti-focal adhesion kinase (FAK) mAb 2A7 and were identified one as p125FAK and the other as a coprecipitating 130-kDa protein. The p125FAK is connected to the Ras/MAPK pathway and is also implicated in TCR/CD3 signalling in T cell. Another protein phosphorylated by OT in RL12-NP was identified as paxillin, a 68-kDa protein localised at focal adhesion sites and associated with p 125FAK. These results indicate that phosphorylation of focal adhesion kinase may be induced in pre-T cell by thymic OT.

Disciplines :

Endocrinology, metabolism & nutrition
Immunology & infectious disease

Author, co-author :

Martens, Henri ; Université de Liège - ULiège > Centre d'immunologie

Kecha, Ouafae; Université de Liège - ULiège > Centre d'Immunologie

Charlet-Renard, Jeanne de Chantal ; Université de Liège - ULiège > Endocrinologie clinique

Defresne, Marie-Paule ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie

Geenen, Vincent ; Université de Liège - ULiège > Centre d'immunologie - Embryologie

Language :

English

Title :

Neurohypophysial peptides stimulate the phosphorylation of pre-T cell focal adhesion kinases

Publication date :

1998

Journal title :

Neuroendocrinology

ISSN :

0028-3835

eISSN :

1423-0194

Publisher :

Karger, Basel, Switzerland

Volume :

Issue :

Pages :

282-289

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 28 August 2009

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