Article (Scientific journals)
Neurohypophysial peptides stimulate the phosphorylation of pre-T cell focal adhesion kinases
Martens, Henri; Kecha, Ouafae; Charlet-Renard, Jeanne de Chantal et al.
1998In Neuroendocrinology, 67 (4), p. 282-289
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Keywords :
Oxytocin; Vasopressin; Thymus; Map kinases; Neuroimmune interactions
Abstract :
[en] Thymic oxytocin (OT) behaves as a cryptocrine signal targeted at the outer surface of thymic epithelial cell plasma membrane from where OT is able to interact with neurohypophysial peptide receptors expressed by pre-T cells. Immature T cells bear a receptor of the V1 subtype, while OT receptors are predominantly expressed by cytotoxic CD8+ lymphocytes. In both T cell types, neurohypophysial peptide receptors transduce OT via the phosphoinositide pathway. Protein tyrosine phosphorylation is an early event of T cell activation. Western blots of murine pre-T cells (RL12-NP line) proteins probed with anti-phosphotyrosine (PY-20) revealed a great number of proteins the phosphorylation of which increased either with OT or vasopressin treatment. Two were immunoprecipitated with anti-focal adhesion kinase (FAK) mAb 2A7 and were identified one as p125FAK and the other as a coprecipitating 130-kDa protein. The p125FAK is connected to the Ras/MAPK pathway and is also implicated in TCR/CD3 signalling in T cell. Another protein phosphorylated by OT in RL12-NP was identified as paxillin, a 68-kDa protein localised at focal adhesion sites and associated with p 125FAK. These results indicate that phosphorylation of focal adhesion kinase may be induced in pre-T cell by thymic OT.
Disciplines :
Endocrinology, metabolism & nutrition
Immunology & infectious disease
Author, co-author :
Martens, Henri ;  Université de Liège - ULiège > Centre d'immunologie
Kecha, Ouafae;  Université de Liège - ULiège > Centre d'Immunologie
Charlet-Renard, Jeanne de Chantal ;  Université de Liège - ULiège > Endocrinologie clinique
Defresne, Marie-Paule ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie
Geenen, Vincent ;  Université de Liège - ULiège > Centre d'immunologie - Embryologie
Language :
English
Title :
Neurohypophysial peptides stimulate the phosphorylation of pre-T cell focal adhesion kinases
Publication date :
1998
Journal title :
Neuroendocrinology
ISSN :
0028-3835
eISSN :
1423-0194
Publisher :
Karger, Basel, Switzerland
Volume :
67
Issue :
4
Pages :
282-289
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 28 August 2009

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